Figure 3.

Iboxamycin displays strong PAE against L. monocytogenes. To determine the time taken for antibiotic treated L. monocytogenes strains to resume growth after a 2 h antibiotic treatment, exponentially growing type strains; 10403S (a), EDG-e (b) or VgaA-deficient EDG-e Δlmo0919 (c) were treated with 4x MIC of either iboxamycin, clindamycin, lincomycin, or no antibiotic as control, for 2 h. Cells were then diluted by 100-fold to remove the antibiotic, and samples taken every 2 h subsequently for viability counting. All experiments were carried out in MH-F broth at 37°C with shaking at 180 rpm, data points are from three biological replicates and standard deviation is indicated with error bars.