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. 2022 May 26;13:890622. doi: 10.3389/fimmu.2022.890622

Table 2.

Binding parameters for interaction of nanoparticles with bNAb CR6261 measured by SPR and MST.

Immunogen Technique Ligand kon (M-1s-1) koff (s-1) KD (nM)
MsDps2- pH1HA10 SPR CR6261 4.5 ± 0.53×105 1.4 ± 0.13×10-3 3.2 ± 0.5
MsDps2- pH1HA10 MST CR6261 1.1 ± 0.5
MsDps2-pH1HA10 SPR CR9114 3.9 ± 0.3×105 1.7 ± 0.1×10-3 4.4 ± 0.3
Encapsulin-pH1HA10 SPR CR6261 1.7 ± 0.3×105 9.6 ± 0.1×10-5 0.5 ± 0.1
Encapsulin-pH1HA10 MST CR6261 9.1 ± 3.1
Encapsulin-pH1HA10 SPR CR9114 1.1 ± 0.1×105 6.4 ± 0.18×10-3 5.5 ± 1.3
pH1HA10-Ferritin SPR CR6261 3.1 ± 0.5×104 No dissociation No dissociation
pH1HA10-Ferritin MST CR6261 6 ± 1.2