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. 2022 May 26;10:895284. doi: 10.3389/fcell.2022.895284

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Copyright © 2022 Parseh, Khosravi, Fazel, Ai, Ebrahimi-Barough, Verdi and Shahbazi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative H&E staining of colon cancer organoid and patient tumor. (A) Well-differentiated adenocarcinoma. (B) Moderately differentiated adenocarcinoma. Histological differentiation pattern analysis indicated tumor organoids retain patient tumor histopathological characteristics. In both paraffin-embedded sections from the original tumor and cultured organoids from the same patient, well-differentiated and moderately differentiated adenocarcinoma were seen. Scale bar: 50 μm, ×400 magnification.