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Colon cancer organoids differentiation status. (A) PAS-positive goblet cells (red arrows). (B) AB-positive goblet cell (green arrows). (C) Secretion process of goblet cell mucus into the lumens of organoids (black arrow). (D) IHC staining for alkaline phosphatase. As expected, Wnt activity strongly inhibits enterocyte differentiation. Scale bar: 50 μm and 100 μm, ×400 and ×200 magnification.
