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. 2022 May 26;10:895284. doi: 10.3389/fcell.2022.895284

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Copyright © 2022 Parseh, Khosravi, Fazel, Ai, Ebrahimi-Barough, Verdi and Shahbazi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization and activation status of NK cells. (A) The population of CD56 + CD3-NK cells after MACS isolation. (B) The activation status of IL-2-activated NK cells by NKG2D and CD69 markers. (C) The IFN-γ concentration of IL-2-activated NK cells conditioned medium (NK-CM). (D) The TNF-α concentration of IL-2-activated NK-CM. The immunostimulatory cytokines level of IL-2-activated NK-CM was significantly increased than the control group (fresh NK cells culture medium). (E) Soluble FasL and TRAIL are present in activated NK-CM determined by western blotting. Data presented as means ± standard error (M± SE). Statistical significance was determined using an unpaired t-test with *p < 0.05. ns: non-significant.