FIGURE 2.

Piezo1 agonism with Yoda-1 induces calcium signaling in MO3.13 oligodendrocytes. (A) Traces calculated from 50 s recordings show changes in Ca²⁺ levels overtime after stimulation with Piezo1 agonist Yoda-1 (20 μM) and no increase after HBSS (ctrl). Peak from t = 10 s to t = 24 s, AUC = 18.19 ± 1.49. Traces are shown as mean ± SEM of N = 4 independent experiments. (B) Corresponding bar graph shows increased Ca²⁺ signaling after receptor stimulation with agonist Yoda-1. Data presented as mean ± SEM, unpaired t-test, ****p < 0.0001, N = 4 independent experiments. (C) Representative images taken from microscope recordings show basal levels of Ca²⁺, followed by induction of Ca²⁺ after addition of Yoda-1 or HBSS (ctrl), and subsequent recovery. Scale 100 μm.