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. 2022 May 26;16:914985. doi: 10.3389/fncel.2022.914985

FIGURE 3.

FIGURE 3

Inhibition of Piezo1 induces MO3.13 oligodendrocyte migration. (A) Representative images and a corresponding bar graph show increased oligodendrocyte migration after inhibition of Piezo1 signaling for 24 h with GsMTx4 (500 nm) (208.4 ± 8.21% vs. control). Agonism of Piezo1 with Yoda-1 (40 μm) induced opposite effects (58 ± 3.82% vs. control). Data presented as mean ± SEM, One-way analysis of variance and Tukey’s multiple comparisons test, ***p < 0.001, N = 8. Scale 25 μm. (B) The MTT cell proliferation assay showed significantly increased cell proliferation after 72 h of Piezo1 inhibition with GsMTx4 (224.8 ± 7.50% vs. control) with no differences compared to the 24 and 48 h controls. Activation of the receptor with Yoda-1 significantly reduced cell proliferation after 48 (110.8 ± 15.58% vs. control) and 72 h (139.0 ± 3.34% vs. control). Data presented as mean ± SEM, One-way analysis of variance and Sidak’s multiple comparisons test, *p < 0.05, **p < 0.01, ***p < 0.001, N = 4 independent experiments.