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. 2022 May 26;16:914985. doi: 10.3389/fncel.2022.914985

FIGURE 4.

FIGURE 4

Expression of Piezo1 decreases in MO3.13 oligodendrocytes during maturation. (A) The mRNA expression of Piezo1 receptor in MO3.13 oligodendrocytes is downregulated already after 24 h of both, serum-free media (SFM) (28 ± 16% SFM 24 h vs. FBS) and SFM supplemented with PMA (8 ± 4% PMA 24 h vs. FBS). At 48 h of treatment with PMA (data not collected for 48 h SFM) Piezo1 mRNA briefly increases (45 ± 8% PMA 48 h vs. FBS, p = 0.0522) indicating a temporary upregulation of Piezo1 signaling during oligodendrocyte maturation. Data presented as mean ± SEM, One-way analysis of variance with Sidak’s multiple comparison’s test, p = 0.013, N = 3 independent experiments. **p < 0.01 (B) SFM (365 ± 20% SFM 72 h vs. FBS) and PMA (314 ± 46% OMA 72 h vs. FBS) treatment induce MO3.13 oligodendrocyty maturation. Data presented as mean ± SEM, One-way analysis of variance with Sidak’s multiple comparison’s test, p < 0.0001, N = 4 independent experiments. ***p < 0.001, ****p < 0.0001 (C) Representative WB image of OPC marker PDGFRα shows steadily decreasing levels of PDGFRα protein in MO3.13 oligodendrocytes stimulated with PMA or cultured in SFM. Data presented as mean ± SEM, One-way analysis of variance with Dunnett’s multiple comparison’s test, p < 0.0026, N = 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.