FIGURE 5.

Bench-top assessment focusing on cage zones. We analyzed home-cage based activities per zone, assessing the duration the animals spent in predefined areas of the home cage. Concerning the zone “drinking,” there was a significant effect of genotype and a relevant genotype by sex interaction in prepubescent mice (A), showing that male Gria1^–/– mice spent more time in the zone “drinking” than age-matched male wild-types. In sexually mature mice (B), we did not detect significant group differences for the time spent in the zone “drinking.” Prepubescent Gria1^–/– mice spent significantly more time in the zone “feeding” than age-matched wild-types (C). This was also the case for sexually mature Gria1^–/– mice (D). Considering the duration spent in the center of the cage, genotypes were statistically similar in prepubescent (E) and in sexually mature (F) mice. ANOVA, followed by FDR correction or Bonferroni multiple comparison post hoc tests. n = 8–10 per genotype per sex. *p < 0.05. Error bars indicate the standard error of the mean (SEM).