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Generation and isolation of MitoT-expressing cells
Mouse embryonic fibroblasts were transfected with pCDNA3-MitoT-eGFP vector, cultured and harvested as explained in the text. Antibiotic-resistant cells were then profiled and processed by FACS. Importantly, FACS profiling revealed two distinct cell populations with low (GFPlo) and high (GFPhi) eGFP expression. Cells from the GFPlo group were confirmed to be the desired cell population and collected for further analyses.
