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. 2022 Jun 14;3(3):101454. doi: 10.1016/j.xpro.2022.101454

Figure 4.

Figure 4

Visualization of MitoT-GFP expression, its colocalization with mitochondria, and ultrastructure analysis

(A) Collected MitoT-eGFP-expressing cells (GFPhi and GFPlo) were treated with MitoTracker Deep Red dye (MTR) and live-imaged by confocal fluorescent microscopy to ensure proper mitochondrial localization of the MitoT-eGFP synthetic protein. Scale bar, 20 μm.

(B) Representative TEM images of OMA1 KO and OMA1 KO_MitoT cells that have been treated with an uncoupler CCCP or left untreated. Induced expression of MitoT in OMA1 KO cells mitigates abnormal mitochondrial cristae morphology that becomes visible upon uncoupling. Scale bar, 0.4 μm. Images adopted from Viana et al. (2021).