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. 2022 May 31;144(23):10151–10155. doi: 10.1021/jacs.2c03486

Figure 3.

Figure 3

Effect of pH, buffer and catalyst on imidazolone formation and hydrolysis. Time courses to show the (A) formation of imidazolone 4A from amidine 3A′ (25 mM) at rt and pH 10 or pH 7, with and without phosphate buffer (PB, 100 mM); (B) formation of peptide 5A′ from 3A′ (25 mM) at r.t and pH 10 or pH 7, with and without PB (100 mM); (C) formation of 4A from 3A′ (25 mM) and 4G from 3G′ (25 mM) at rt and pH 9, with and without 6b (100 mM); (D) formation of peptides 5A′ from 3A′ (25 mM) and 5G′ from 3G′ (25 mM) at rt and pH 9, with and without 6b (100 mM).