Table 2. Hydrolysis of Amidine 3Aa.
entry | pH | temp, °C | buffer (500 mM) | time | 3A, % | 5A, % | 2A, % |
---|---|---|---|---|---|---|---|
1 | 7 | 20 | 30 days | 100 | 0 | 0 | |
2 | 7 | 80 | 18 h | 34 | 15 | 36 | |
3 | 7 | 80 | PB | 18 h | 11 | 31 | 52 |
4 | 9 | 80 | BB | 18 h | 0 | 38 | 61 |
PB = phosphate buffer, BB = borate buffer.