Table 3.

Some limitations and solutions in the application of CRISPR/Cas9 gene editing

Limitations	Solutions	References
Off-target mutagenesis	Correct design of sgRNA D10A-mutated Cas9 SpCas9-HF1 and eSpCas9	[188–190]
Identification of the PAM region	SpCas9-NG and xCas9 variants	[191]
P53 activation and apoptosis	Cas9 variants with the ability to create single-strand breaks	[191]
Anti-SpCas9 antibodies	Campylobacter jejuni Cas9 (CjCas9)	[191, 192]
Stimulation of immune responses in delivery by viral vector	Using other delivery method such as nanoparticle	[193]