Fig. 2.

TAMs promote epithelial–mesenchymal transition in BT549 and HCC1937 cells. A Representative images of THP1 cells treated with IL-4 (20 ng/mL) for 48 h. B Flow cytometry analysis of the expression of CD206 and CD163 in M0 and M2 macrophages. C BT549 and HCC1937 cells were cultured with M2-CM or M0-CM, cell invasion were measured by transwell assay (up). Histogram showing the number of invasive BT549 and HCC1937 cells in transwell invasion assays (down). Scale bar = 100 μm. D The migratory ability of BT549 and HCC1937 cells treated with M0-CM or M2-CM was measured by wound healing assays. Scale bar = 100 μm. E BT549 and HCC1937 cells were cultured with M2-CM or M0-CM, the expression of E-cadherin, N-cadherin, vimentin, and Snail were measured using western blotting. N = 3. **p < 0.01