Fig. 2.

Changes in the viability of A549 cells in response to SIV. A A549 cells were infected of SIV at an MOI of 0.01, 0.1, 1 MOI or treated with erastin (10 μM) for 24 h, and cell viability was measured using the CCK-8 method. B A549 cells were treated with Fer-1 (1 μM) or not 1 h before infection with SIV (MOI of 1), and cell viability was measured at 24 h.p.i.. Erastin (10 μM) was used as the positive control, and Fer-1 (1 μM) was used as the ferroptosis inhibitor. C Morphological changes in cells were observed after treatment with or without Fer-1 (1 μM) 1 h before cells were infected with SIV (MOI of 1). Images were captured at a magnification of 20 × 24 h after treatment by microscopy. The error bars represent the SD of the means on the basis of three independent experiments. (*p < 0.05, **p < 0.01)