Fig. 6.

C3aR blockade inhibited APT2/DHHC7 palmitoylation-mediated STAT3 phosphorylation in the prefrontal cortex. The levels of APT2 (A), DHHC7 (B) and pSTAT3 (C) were decreased after C3aR blockade. Representative photographs (D) of Iba1(red)/pSTAT3(green)/APT2(purple)/DAPI(blue) and the co-localized ratio (E) of pSTAT3 and APT2. Representative photographs (F) of Iba1(red)/DHHC7(green)/STAT3(purple)/DAPI(blue) and the co-localized ratio (G) of STAT3 and DHHC7. C3aR blockade decreased proinflammatory cytokines both in mRNA expression (H–J) and protein levels (K–M). Data were obtained from 5–6 samples per group. ^#P < 0.05 and ^##P < 0.01 versus the Control-vehicle group. ^*P < 0.05 and ^**P < 0.01 versus the CUMS-vehicle group