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. 2022 Jun 3;13:828949. doi: 10.3389/fimmu.2022.828949

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Copyright © 2022 Sehgal, Maiwall, Rajan, Islam, Baweja, Kaur, Kumar, Ramakrishna, Sarin and Trehanpati

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of MDSCs in patient groups. (A) Sequential gating strategy for identification of MDSCs and their subsets using flow cytometry. MDSCs are characterized as CD11b⁺CD33⁺HLADR^−ve. (B) Scatter dot plot shows %frequency of MDSCs, G-MDSCs, and M-MDSCs. (C) ROC curve shows high specificity and sensitivity of MDSCs and G-MDSCs in sepsis patients compared to w/o sepsis patients. Results are expressed as the mean ± SD. (B) One-way ANOVA/Kruskal–Wallis test followed by probability adjustment by the Mann–Whitney. (C) ROC curve via SPSS. MDSCs, myeloid-derived suppressor cells; ROC, receiver operating characteristic.