Figure 2.

Identification of CD4 T cells, their subsets, and Tregs in patient groups. (A) Sequential gating strategy for identification of CD4 and its subsets using CCR7 and CD45RA, i.e., T_CM, T_NAIVE, T_EM, and T_EMRA. Scatter dot plot shows %frequency of CD4 T cells in patient groups. (B) Scatter dot plot shows %frequency of T_CM, T_NAIVE, T_EM, and T_EMRA. (C) Correlation between %CD4 T_CM and total bilirubin in sepsis group. (D) UMAP visualization of pooled lymphocytes of HCs and patients w/o and with sepsis for characterization of Tregs. (E) Scatter dot plots show %frequency of Tregs. (F) ROC curve shows high specificity and sensitivity of Tregs in sepsis group compared to w/o sepsis group. Also, correlation of %Tregs with MELD in sepsis group. Results are expressed as the mean ± SD. (A, B, E) One-way ANOVA/Kruskal–Wallis test followed by probability adjustment by the Mann–Whitney. (C–F) Scatter diagram showing the regression line. UMAP, Uniform Manifold Approximation and Projection; HCs, healthy controls; ROC, receiver operating characteristic; MELD, model for end-stage liver diseases.