Figure 3.

MDSCs action on T-cell functionality and Tregs. (A) Fold-change expression of IL-10, Arg1, and iNOS in sorted MDSCs (white color) and monocytes (black color) in the patient groups through qRT-PCR. (B) %Apoptosis using PI−veAnnexin V+ve and (C) %Proliferation using CFSE through flow cytometry in T cells cultured alone (black color) and with MDSCs (white color) and monocytes (gray color) in the patient groups. Expression of %FOXP3⁺ on CD4⁺ T cells (D) under Th0 condition and (E) under Th17-proliferating condition was observed in the patient groups when T cells were cultured alone (black color) and with MDSCs (white color). (F) Expression of %FOXP3⁺ on CD4⁺ T cells in T cells cultured with MDSCs along with stimulations, i.e., rTGF-β, l-NMMA, and nor-NOHA in the patient groups. HCs (black color), without sepsis (gray color), and with sepsis (white color). Results are expressed as the mean ± SD. (A) One-way ANOVA/Kruskal–Wallis test followed by probability adjustment by the Mann–Whitney. (B–E) Mann–Whitney/t-test within the patient groups. (F) Kruskal–Wallis test within the group along with the multiple comparisons. MDSC, myeloid-derived suppressor cell; CFSE, carboxyfluorescein succinimidyl ester.