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. Author manuscript; available in PMC: 2022 Jun 17.
Published in final edited form as: Exp Eye Res. 2020 Nov 16;202:108361. doi: 10.1016/j.exer.2020.108361

Table 4.

Target therapeutic genes, study models, species, and delivery techniques are outlined by various vector platforms and corneal disorders.

rAdenovirus Vector
Corneal Disorder Therapeutic Gene Model Species Delivery References
Corneal Graft Rejection Interleukin 4, 12 In Vivo Rat IP Ritter et al. (2007)
Interleukin 10 Ex Vivo Sheep IP Yuan et al. (2013)
Interleukin 12 In Vivo Rat IP Yuan et al. (2013)
Inducible T-cell Co-stimulator (ICOS) Ex Vivo Rat Topical Gong et al. (2006)
T-Lymphocyte-Associated Antigen 4 In Vivo Rat IV Nguyen et al. (2013); Yuan et al. (2013)
NGF In Vivo Rat IP Nguyen et al. (2013)
Corneal Scarring, Haze, and Wound Healing Type II TGFβ Receptor In Vivo, Ex Vivo Mice, Rabbit, Human IM, Topical Sharma et al. (2012)
BMP7 In Vivo Mice Topical Gupta et al. (2018); Saika et al. (2005a)
Smad7 In Vivo Mice Topical Saika et al. (2005b)
Corneal Alkali Burn Injury Smad7 In Vivo Mice Topical Saika et al. (2005b)
BMP7 In Vivo, In Vitro Mice, Human Topical Gupta et al. (2018); Saika et al. (2005a)
PPAR γ In Vivo Mice IV Li et al. (2014)
Corneal Neovascularization VEGFR FLT-1 In Vivo Mice IV Lai et al. (2005)
Decorin In Vivo Rabbit Topical Chaudhary et al. (2014)
Chloramphenicol acetyltransferase In Vivo Rabbit Lamellar Flap Mohan et al. (2003)
β-Galactosidase In Vivo Mice, Rabbit IS Larkin et al. (1996); Selvam et al. (2006)
sFlt-1 In Vivo Rat IC Lai et al. (2005)
Vasohibin-1 In Vivo Mice Subconj. Zhou et al. (2010)
Herpes Simplex Virus Keratitis HGF c-met In Vitro Human Topical Gupta et al. (2018)
HSV Glycoproteins (gB, gC, gD, gE, gI) In Vivo Mice, Rabbit IP, IM, Subconj Elbadawy et al. (2012)
Lacrimal Gland, Dry Eye, TNF α Inhibitor In Vivo Rabbit Lacrimal Gland Selvam et al. (2006)
Sjogren syndrome Interleukin 10 In Vivo Rabbit Lacrimal Gland Selvam et al. (2006)
Conjunctival Scarring Smad7 In Vivo Mice Topical Saika et al. (2005b)
PPAR γ In Vivo Mice IV Li et al. (2014)
rRetrovirus Vector
Corneal Disorder Therapeutic Gene Model Species Delivery References
Corneal Scarring, Haze, and Wound Healing HSV Thymidine Kinase In Vivo Rabbit Topical Selvam et al. (2006)
Dom-Neg. Cyclin G1 In Vivo Rabbit Topical Behrens et al. (2002)
Dom-Neg. p38MAPK In Vivo Mice Topical Behrens et al. (2002)
rLentivirus Vector
Corneal Disorder Therapeutic Gene Model Species Delivery References
Corneal Graft Rejection Bcl-xL In Vitro Human Liu et al. (2005)
Corneal Neovascularization Endostatin/Kringle 5 Plasmin In Vivo Rabbit Subconj. Murthy et al. (2003)
rHerpes Simplex Virus Vector
Corneal Disorder Therapeutic Gene Model Species Delivery References
Corneal Endothelial Cell Wound Healing LacZ Ex Vivo Rabbit, Human Topical Hudde et al. (2000)
LacZ In Vivo Monkey IC Liu et al. (1999)
LacZ In Vivo Mice IV, IC Spencer et al. (2000)
rAdeno-associated Virus Vector
Corneal Disorder Therapeutic Gene Model Species Delivery References
Corneal Scarring, Haze, and Wound Healing Decorin In Vivo, Ex Vivo Mice, Rabbit, Human Topical Mohan et al. (2019); Mohan et al. (2011b)
Smad7 Ex Vivo Rabbit, Horse Topical Saika et al. (2005b); Marlo et al. (2018); Gupta et al. (2017)
Type II TGFβ Receptor In Vivo, In Vitro Mice, Rabbit, Human IM, Topical Sharma et al. (2012)
Corneal Neovascularization sFlt-1 In Vivo, Ex Vivo Mice, Monkey IC Lai et al. (2005)
Decorin In Vivo, Ex Vivo Rabbit, Human Topical Mohan et al. (2011b), 2011c; Chaudhary et al. (2014)
PEDF In Vivo, Ex Vivo Rabbit, Human Topical Yu et al. (2010)
VEGFR FLT-1 In Vivo Mice IS Lai et al. (2005); Li et al. (2014); Mohan et al. (2011a)
EGFP In Vivo Topical Bosiak et al. (2012); Buss et al. (2010)
Corneal Alkali Burn Injury Canine, Horse
Angiostatin In Vivo, Ex Vivo Rat, Human Subconj. Cheng et al. (2007)
Other Non-Viral Vectors/Methods
Corneal Disorder Therapeutic Gene Model Species Delivery References
Corneal Graft Rejection Tissue Plasminogen Activator In Vivo Rat Electroporation Murthy et al. (2003)
Corneal Neovascularization Kringle 5 Plasmin In Vivo Rabbit Electroporation Murthy et al. (2003)
Interleukin 10 In Vivo Mice Topical Nguyen et al. (2013)
Interleukin 12 In Vivo Mice Topical Nguyen et al. (2013)
VEGFR FLT-1 In Vivo Mice IS Lai et al. (2005)
sFlt-1 In Vivo Mice IS Lai et al. (2005); Li et al. (2014)
FLT23K, FLT24K In Vivo Mice IS Lai et al. (2005); Li et al. (2014)
Herpes Simplex Virus Keratitis HSV Glycoproteins (gB, gC, gD, gE, gI) In Vivo Mice, Rabbit IP, IM, SubConj Elbadawy et al. (2012)
Interleukin 2 In Vivo Mice IV Elbadawy et al. (2012)
Interleukin 4 In Vivo Mice IV Elbadawy et al. (2012)
Interleukin 10 In Vivo Mice IV Elbadawy et al. (2012)
Nanoparticle Vector
Corneal Disorder Therapeutic Gene Model Species Delivery References
Corneal Scarring, Haze, and Wound Healing BMP7 In Vivo Rabbit, Human Topical Gupta et al. (2018); Chaurasia et al. (2015)
Type II TGFβ Receptor In Vivo, Ex Vivo Mice, Rabbit, Human IM, Topical Sharma et al. (2012)
PGLA In Vivo Rabbit Topical Chaurasia et al. (2015)
Smad7 In Vivo Rabbit Topical Saika et al. (2005b)
Corneal Neovascularization FLT23K In Vivo Mice IS Lai et al. (2005)
Gene Editing by CRISPR/Cas9
Corneal Disorder Therapeutic Gene Model Species Delivery References
Corneal Endothelial Cell Wound Healing SOX2 In Vivo Rats Electroporation Chang et al. (2018)
Fungal Keratitis Ura3 repair template Ex Vivo Human Transformation Lightfoot and Fuller (2019)
Congenital Aniridia PAX6 of LSC In Vitro Human Transformation Roux et al. (2018)

(BMP7: Bone Morphogenic Protein 7; HGF: Hepatocyte Growth Factor; HSV: Herpes Simplex Virus; PPAR γ: Peroxisome Proliferator-Activated Receptor gamma; TGFβ: Transforming Growth Factor Beta; TNFα: Tumor Necrosis Factor alpha; VEGF: Vascular Endothelial Growth Factor Receptor; IC: Intracameral; IM: Intramuscular; IP: Interpleural; IS: Intrastromal: IV: Intravenous; Subconj: Subconjunctival).