Extended Data Fig. 7.
APEX-SDC145,47 glycoconjugation yields and additional proximity labeling data (A) Weak anion exchange (WAX) traces demonstrating successful conjugation of azHEP to A-SDC145,47 (grey) and formation of the more anionic product A-SDC145,47HEP (blue). CuAAC performed at quantitative yields. (B) Dose dependent fluorescence generated after live cell proximity labeling with cell surface (10 μM cholPEGNTA) A-SDC145,47 at 5 μM, 2 μM and 1 μM. Biotinylation is detected by Cy5-streptavidin (pink) with fluorescence mostly localized to cell surfaces. Representative images from three biological replicates.