TABLE 2.
Equilibrium metal speciation of media during measurement of whole-cell sMMO activity
| Metal speciation | Result in medium (chelating agent)
|
||
|---|---|---|---|
| NMS (EDTA) | M2M (pyrophosphate) | M2M (EDTA) | |
| Total Cu (μM) | 0–0.13 | 0–0.08 | 0.01–0.2 |
| Chelated Cu (%) | 97.7 | 99.9 | 100 |
| Precipitated Cu (%) | 2.2 | 0 | 0 |
| Free Cu (%) | 0.15 | 0.06 | <10−4 |
| pCua | 10.3–9.8 | 10.8–10.3 | >11.6 |
| Total Fe (μM) | 9.4 | 1.2 | 1.0 |
| Soluble Fe (%) | 95 | 8 | 20 |
| Precipitated Fe (%) | 5 | 92 | 80 |
a
Free copper concentrations at equilibrium, reported as the negative log of [Cu2+].