Figure 2.

Fat mass and obesity-associated protein (FTO) inhibits papillary thyroid carcinoma (PTC) proliferation, migration, and invasion in vitro and in vivo. (A) Construction of the FTO overexpression model of TPC-1 and the FTO silencing model of KTC-1 as verified by western blot. (B) Cell proliferation was applied to evaluate the proliferation abilities of three PTC cell lines with knockdown or overexpression of FTO. (C) Wound healing assays showed the suppressed migration of PTC cells, with knockdown or overexpression of FTO, respectively, following FTO overexpression. In contrast, FTO knockdown promoted the migration (wound closure) of PC cells. (D) Transwell migration and invasion experiment. (E) Overexpression of FTO significantly increased cell apoptosis in PTC cells by fluorescence-activated cell sorting. (F) Macroscopic image of the tumor size in a xenograft mouse model in which the mice were injected with control and KTC-1-shFTO cells (stably knockdown FTO). The knockdown of FTO dramatically promoted PTC tumor growth in an orthotopic xenograft mouse model. *, P<0.05.