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. 2022 Apr 27;2(5):100129. doi: 10.1016/j.xgen.2022.100129
REAGENT or RESOURCE SOURCE IDENTIFIER
Deposited data

PacBio HiFi/CCS Sequel II Wagner et al.10 SRA: SRX7083054 to SRA: SRX7083057, SRA: SRX8136474 to SRA: SRX8136477, SRA: SRX8137018 to SRA: SRX8137021
Challenge sequencing datasets https://doi.org/10.18434/mds2-2336

Experimental models: Cell lines

Son of Ashkenazi Jewish ancestry (HG002) NIST Office of Reference Materials; Coriell/NIGMS; PGP NIST RM8391/RM8392; GM24385; RRID:CVCL_1C78
Father of Ashkenazi Jewish ancestry (HG003) NIST Office of Reference Materials; Coriell/NIGMS; PGP NIST RM8392; GM24149; RRID:CVCL_1C54
Mother of Ashkenazi Jewish ancestry (HG004) NIST Office of Reference Materials; Coriell/NIGMS; PGP NIST RM8392; GM24143; RRID:CVCL_1C48

Software and algorithms

hap.py https://github.com/Illumina/hap.py
seqtk https://github.com/lh3/seqtk
Code used to analyze challenge results and benchmarking results files This paper https://github.com/usnistgov/giab-pFDA-2nd-challenge
https://doi.org/10.5281/zenodo.6384789

Other

Sequence data, analyses, and resources related to the NIST Genome in a Bottle Consortium samples in this manuscript This paper https://www.nist.gov/programs-projects/genome-bottle
GIAB stratifications used for benchmarking This paper https://doi.org/10.18434/mds2-2499, also available at https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/genome-stratifications/v2.0/ code to generate stratifications available at https://github.com/genome-in-a-bottle/genome-stratifications/releases/tag/v2.0