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. 2022 Jun 17;17(6):e0270031. doi: 10.1371/journal.pone.0270031

Music listening and stress recovery in healthy individuals: A systematic review with meta-analysis of experimental studies

Krisna Adiasto 1,*, Debby G J Beckers 1, Madelon L M van Hooff 1, Karin Roelofs 1,2, Sabine A E Geurts 1
Editor: Urs M Nater3
PMCID: PMC9205498  PMID: 35714120

Abstract

Effective stress recovery is crucial to prevent the long-term consequences of stress exposure. Studies have suggested that listening to music may be beneficial for stress reduction. Thus, music listening stands to be a promising method to promote effective recovery from exposure to daily stressors. Despite this, empirical support for this opinion has been largely equivocal. As such, to clarify the current literature, we conducted a systematic review with meta-analysis of randomized, controlled experimental studies investigating the effects of music listening on stress recovery in healthy individuals. In fourteen experimental studies, participants (N = 706) were first exposed to an acute laboratory stressor, following which they were either exposed to music or a control condition. A random-effects meta-regression with robust variance estimation demonstrated a non-significant cumulative effect of music listening on stress recovery g = 0.15, 95% CI [-0.21, 0.52], t(13) = 0.92, p = 0.374. In healthy individuals, the effects of music listening on stress recovery seemed to vary depending on musical genre, who selects the music, musical tempo, and type of stress recovery outcome. However, considering the significant heterogeneity between the modest number of included studies, no definite conclusions may currently be drawn about the effects of music listening on the short-term stress recovery process of healthy individuals. Suggestions for future research are discussed.

Introduction

The prevalence of stress-related diseases worldwide has seen no decrease over the previous decade [1, 2], as stress has become so pervasive in daily life that our physiological systems are under constant pressure to cope with various stressors [3]. Stress recovery has been introduced as a process which may mitigate the adverse consequences of frequent stress exposure [4, 5]: effective stress recovery on a daily basis may prevent the occurrence of blunted or exaggerated stress responses that over time develop into various physiological and psychological disorders, such as cardiovascular and cerebrovascular disease, hypertension, burnout, and depression [2, 58].

Given the importance of effective stress recovery from exposure to daily stressors, research on potential means to promote stress recovery has experienced significant growth [5]. Various activities have been proposed that may lead to better stress recovery, one among them being music listening. Music listening may have a modulatory effect on the human stress response [9]. Furthermore, given that music is readily available through online streaming services, music listening stands to be a time- and cost-effective method to facilitate daily stress recovery. Indeed, a recent meta-analysis of 104 randomized controlled trials on the effects of music concluded that music-based interventions have a positive impact on both physiological (d = .380, 95% CI [0.30–0.47]) and psychological (d = .545, 95% CI [0.43–0.66]) stress-related outcomes [10]. However, a large proportion of studies included in this meta-analysis were conducted in medical or therapeutic settings, and the included music-based interventions encompassed not only music listening but also music therapy. Thus, a more specific review to determine whether music listening alone is beneficial for the recovery of healthy individuals outside medical and therapeutic settings seemed justified.

To expand on the above considerations: stressors in medical or therapeutic settings (e.g., treatment anxiety, pregnancy, and labor) and their subsequent stress recovery processes can be difficult to generalize to more daily settings [1013]. Next, with regards to music-based interventions, music listening simply involves listening to a particular song, while music therapy is characterized by the presence of a therapeutic process and use of personal music experiences, and thus must be performed by a trained music therapist [14]. In practice, music therapy may not only involve music listening, but also music playing, composing, songwriting, and interaction with music [10, 14]. The effects of music therapy on stress appear to be more consistent compared to music listening [10, 15, 16]. Studies on music listening and stress recovery in healthy individuals are indeed equivocal: though music listening is considered beneficial for physiological stress recovery, several studies have reported no differences in heart rate, heart rate variability, respiration rate, blood pressure, or cortisol recovery between participants who listened to music and those who either sat in silence or listened to an auditory control [1720]. Similarly, although music is notable for its anxiolytic effects, several studies have reported no significant differences in post-stressor anxiety between participants who listened to music and those who did not [3, 18, 21]. Taken together, it is currently difficult to draw definite conclusions about the effects of music listening on stress recovery in healthy individuals, particularly outside medical and therapeutic settings [15, 22].

Therefore, to expand on previous reviews, we opted to conduct a systematic review with meta-analysis on experimental studies in healthy individuals, focusing specifically on the role of music listening in stress recovery. In our review, we focus specifically on experimental studies, under the assumption that greater control over study variables would help reduce between-study heterogeneity. Furthermore, considering the crucial role of stress recovery in preventing the long-term consequences of stress exposure [5, 23], we believe the acute stress responses elicited by laboratory stressors would more closely approximate typical stress responses in daily life. The aim of our review was two-fold: through systematic review, we provide a comprehensive account of experimental studies examining the effect of music listening on stress recovery. Through meta-analysis, we assess the reliability of the effect of music listening on stress recovery, including the extent and impact of publication bias, and weigh-in on outstanding discussions within existing literature.

The stress response

The stress response can be conceptualized as a compensatory reaction aimed at mitigating the potential consequences of a stressor [24, 25]. The stress response is best illustrated by the archetypal ‘fight-flight-freeze’ reaction: in the presence of a stressor, the brain initiates an elegant synergy of neuroendocrine, physiological, and psychological processes that serve to mobilize energy resources and direct attention towards prominent stimuli, with the aim of promoting appropriate and rapid action [26, 27]. During a stress response, the autonomic nervous system (ANS) suppresses parasympathetic activity and promotes sympathetic exertion, resulting in marked increases in heart rate, respiration rate, systolic and diastolic blood pressure, and salivary secretion of the dietary enzyme, alpha-amylase [2731]. These changes are mediated by neuropeptides (e.g., corticotropin-releasing factor) and catecholamines (e.g., norepinephrine, dopamine) [24, 25]. Simultaneous with ANS activity, the hypothalamic-pituitary-adrenocortical (HPA) axis begins a process which leads to a surge of cortisol production in the adrenal cortex [24, 25]. Cortisol acts as a regulator of the stress response, whose effects occur in a temporally specific manner due to variations in corticosteroid receptor affinity and distribution throughout the body [24, 26, 32]. Cortisol may require up to 45 minutes to reach peak concentration levels, during which it binds to high-affinity corticosteroid receptors [24]. This process enables rapid, non-genomic effects that sustain ANS-mediated changes for the duration of the stressor, while suppressing immune system function [3234]. This suppression is visible through lower concentrations of immunoglobulins, such as salivary immunoglobulin-A (s-IgA) [35].

The physiological changes triggered by the ANS and HPA axis are supplemented by psychological changes that motivate adaptive behaviours required to cope with the stressor [25, 27]. For example, the unpleasant feeling one gets when experiencing anxiety and negative affect in response to a stressor is thought to prompt behaviours aimed at reducing these unpleasant states. Since psychological reactions to stressors are contingent on how individuals perceive, evaluate, and react to threats and challenges [36], self-reported measures of stress, anxiety, arousal, and emotion are common in psychological research on stress and its consequences [18, 3739].

Stress recovery

The stress response is considered adaptive when it is short-lived and immediately followed by a period of recovery following stressor cessation. In this period, ANS- and HPA-mediated changes that have occurred in response to a stressor revert to pre-stress baselines [24, 25, 27]. Therefore, stress recovery may be conceptualized as the process of unwinding that is opposite to the neuroendocrine, physiological, and psychological activation that occurs during the stress response [4, 5]. Following a stress response, ANS-mediated changes quickly revert to pre-stress levels within 30 to 60 minutes [26]. This manifests as a restoration of parasympathetic activity, marked by a deceleration of heart rate and respiration rate, lower systolic and diastolic blood pressure, and less activity of salivary alpha-amylase [4, 2831]. This restoration of parasympathetic activity typically precedes any decline in cortisol. Instead, during the same window of time, cortisol levels will have just reached their peak, activating low-affinity corticosteroid receptors [40]. This process is thought to signal the termination of the stress response, as the binding of cortisol to low-affinity receptors inhibits further autonomic activation [24, 26]. As cortisol levels begin to decrease, slow, cortisol-mediated genomic changes are initiated, which directly oppose the rapid effects of catecholamines and the non-genomic effects of cortisol [24, 26]. Following a stressor, these genomic changes may take up to one hour to commence and may continue for several hours [24, 26].

At a psychological level, stress recovery is typically experienced as a reduction of unpleasant states, which is often reflected by lower ratings of self-reported stress, anxiety, and negative affect, along with higher ratings of relaxation and positive affect [5, 15, 18]. However, it is worth noting that persistent, ruminative thoughts about a stressor may delay stress recovery by prolonging the physiological activation that occurs during the stress response [4145]. Indeed, participants who reported higher rumination following a stress task demonstrated poorer heart rate, systolic blood pressure, diastolic blood pressure, and cortisol recovery compared to participants who did not [41, 42, 44, 46, 47].

Music listening and stress recovery

Within the current literature, music listening has frequently been related to various neuroendocrine, physiological, and psychological changes that are considered beneficial for stress recovery [10, 11, 15, 22]. For example, music listening has been associated with lower heart rate [4850], systolic blood pressure [21, 49, 51], skin conductance [17, 19, 52, 53], and cortisol [54, 55] compared to silence or an auditory control condition. Furthermore, music listening has been associated with higher parasympathetic activity [56] compared to silence [3, 37]. Together, these findings suggest that music listening may generate beneficial changes in ANS and HPA axis activity that should be conducive to the stress recovery process [27, 57, 58]. Furthermore, studies have demonstrated that listening to music may influence mood [59, 60]. Indeed, music listening has been associated with lower negative affect [37], higher positive affect [18, 61], and fewer self-reported depressive symptoms [37] compared to silence or an auditory control condition. Music listening has also been associated with lower subjective stress [53, 54], lower state anxiety [37, 48, 49], and higher perceived relaxation [17, 48, 62].

The exact mechanisms underlying the effects of music listening on stress recovery remain to be elucidated. Music-evoked positive emotions are thought to be particularly beneficial for stress recovery, as they may help undo the unfavourable changes wrought by negative emotions during stress, ultimately aiding the stress recovery process [63]. Alternatively, music-evoked emotions may promote a more robust, and thus more adaptive, stress response [61], which may be followed by an equally robust period of stress recovery. Next, it has been theorized that music may act as an anchor that draws attention away from post-stressor ruminative thoughts or negative affective states, thus preventing a lengthening of physiological activation, and facilitating a more regular stress recovery process [45, 64]. Finally, physiological rhythms in our body, such as respiration, cardiovascular activity, and electroencephalographic activity, may become fully or partially synchronized with rhythmical elements perceived in music [6568]. This rhythmic entrainment process is thought to occur via a bottom-up process that originates in the brainstem: salient musical features, such as tempo, pitch, and loudness, are continuously tracked by the brainstem, generating similar changes in ANS activity over time [69, 70]. Indeed, studies have demonstrated that changes in a song’s musical envelope, which represents how a song unfolds over time, are closely followed by proportional changes in blood pressure and skin conductance [52, 65]. Similarly, incremental changes in musical tempo, which represents the speed or pace of a song, were predictive of similar changes in heart rate, blood pressure, and respiration rate [7173]. It is further hypothesized that the physiological changes resulting from rhythmic entrainment may evoke any number of associated emotions via proprioceptive feedback mechanisms [66, 69, 70]. Indeed, higher self-reported entrainment predicted increased positive affect, along with other self-reported emotional responses, such as transcendence, wonder, power, and tenderness [66].

Which music works best?

There are several ongoing discussions about potential moderating effects in the relationship between music listening and stress recovery. We briefly describe these effects below, and later contribute to the discussion through moderator analyses.

Classical music vs. other genres

Classical music is considered the golden standard in many stress management efforts. Indeed, a copious amount of ‘anti-stress’ playlists often feature some selection of classical pieces. To discern which music best promotes stress recovery, studies have contrasted the effects of classical music with other musical genres, including rock [48], jazz and pop [21], and heavy metal [17]. We compare the effects of different musical genres in our moderator analysis.

Instrumental vs. lyrical

It is commonly believed that instrumental, as opposed to lyrical music, would better promote stress recovery. However, several studies have argued that lyrics may act as a stronger distractor compared to the sound of instruments. Thus, lyrical music may be more effective than instrumental music in preventing the prolonged physiological activation that may occur due to ruminative thoughts [17, 18, 74]. We compare the effects of instrumental and lyrical music in our moderator analysis.

Self- vs. experimenter selected

Studies on the effects of music often fail to consider the differential effects of self-selected (i.e., chosen by participants) and experimenter selected (i.e., chosen for participants by the experimenter) music [15]. It is hypothesized that allowing participants to select their own music may be more helpful to promote stress recovery due to a restoration of perceived control [15]. It has also been argued that individuals select music in service of personal self-regulatory goals [64, 75, 76], meaning that individuals know precisely which music to select for them to effectively recover from stress [38, 77]. Furthermore, previous studies have found that listening to self-selected music may help elicit stronger and more positive emotional responses regardless of a song’s valence (positive or negative) and arousal (high or low), possibly due to increased preference and familiarity towards the self-selected music [7880]. In theory, self-selected music should thus be more beneficial compared to experimenter-selected music for the purpose of stress recovery. We compare the effects of self- and experimenter selected music in our moderator analysis.

Fast vs. slow tempo

Several studies have investigated whether listening to music with slower tempo will better facilitate stress recovery compared to music with faster tempo. For example, while listening to an instrumental song, proportional increases and decreases in tempo resulted in similar changes in participants’ heart rate [73]. Similarly, sequential decreases in tempo predicted greater increases in parasympathetic activity compared to sequential increases in tempo [71]. We investigate whether slower tempi differentially influence the effect of music listening on stress recovery in our moderator analysis.

Method

The present review was designed following the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols (PRISMA-P) guidelines [81]. All materials relevant to this review, including: (a) the pre-registered study protocol; (b) an outline of the search strategy; (c) a list of screened articles with rationales for exclusion; (d) the meta-analysis data set with extracted data; and, (e) R code to replicate the analysis reported in this review, are available on the Open Science Framework (https://osf.io/9pxhj).

Study selection

The study selection process is summarized in Fig 1. In April 2021, we conducted a comprehensive literature search for experimental studies on the effect of music listening on stress recovery. The search was conducted using RUQuest, the electronic search system of Radboud University library, which accesses several notable bibliographic databases, including MEDLINE, Wiley Online Library, ScienceDirect, SpringerLink, Taylor and Francis, and JSTOR. The results of this primary search were supplemented with three additional electronic searches in the publication databases of Web of Science, PsycINFO, and PubMed. Appendix A provides a complete description of our search terms. Together, this first step resulted in 3124 articles.

Fig 1. Overview of the study selection process.

Fig 1

Next, the first author (KA) screened all titles and abstracts for studies examining the effects of music listening on stress recovery. If there was any doubt about the eligibility of an article, it was retained for further review. During this initial screening, 3008 articles were excluded. KA then scanned the reference lists of the 116 remaining articles for potentially relevant studies, resulting in an additional three articles. Together, this second step resulted in 119 full-text reports to be assessed for eligibility.

Lastly, KA used the following criteria to assess full-text reports for eligibility:

First, to minimize between-study heterogeneity, and to ensure that included studies investigated the effects of music listening on stress recovery as precisely as possible, studies must employ an experimental design including stress induction, with random assignment of participants to experimental and control conditions. Quasi-experimental studies were included only when they incorporated a control or comparison group. Second, studies should compare music listening to silence or an auditory stimulus (e.g., white noise, audiobooks). To ensure that included studies tested the immediate effect of music listening on stress recovery, exposure to music, silence, or auditory stimuli must occur after the stress induction procedure. Third, to demonstrate this effect, studies must include at least one measure of neuroendocrine (e.g., cortisol), physiological (e.g., heart rate, blood pressure), or psychological (e.g., subjective stress, positive and negative affect) stress recovery outcome. Fourth, given that stress reactivity and recovery responses differ between children and adults, and with consideration to the potential role of music in the prevention of stress-related diseases in adults, studies must include healthy, adult, human participants. Fifth, to improve the generalization of our results in the context of daily stress recovery, studies where stress recovery occurred within a medical or therapeutic context, such as a hospital or operating room, were excluded. Finally, for the purpose of the meta-analysis, means and standard deviations of stress recovery outcomes following stressor cessation must be available. Corresponding authors were contacted when this information was not available. When authors did not or could not provide the required information (e.g., due to data no longer being accessible), outcomes were dropped from the meta-analysis. Following attempts to obtain missing information, the final sample for our review consisted of 14 studies.

Methodological moderators of interest

Several methodological differences were identified between included studies that may moderate the effect of music listening on stress recovery:

Stress induction procedures

Studies utilized a diverse array of stress induction procedures. These include mental arithmetic tasks [e.g., 21], adaptations of the Trier Social Stress Task [e.g., 3], impromptu presentations [49, e.g., 50], unpleasant stimuli [e.g., 82], cognitive tests [e.g., 48], or a CO2 stress task [61]. Stress induction procedures may generally be classified based on the inclusion of a social-evaluative threat (SET) component, which are designed to induce psychosocial stress and have been shown to elicit greater cardiovascular and cortisol responses [83]. In the event of a greater stress response, the effects of music listening on stress recovery may be larger, since there may be a larger window for the stress recovery process to occur. We examined this possibility in our moderator analysis.

Stress induction checks

Stress induction procedures in included studies were not always successful. Given that successful stress induction procedures are crucial to ensure that participants experience some physiological or psychological change they may recover from, in our moderator analysis we examined whether the effect of music listening on stress recovery differed based on the outcome of a study’s stress induction check (manipulation check).

Type of outcome

Studies adopted numerous outcome measures as indicators of stress recovery. These include indicators related to ANS and HPA axis activity, such as heart rate [e.g., 49], heart rate variability [e.g., 3], blood pressure [e.g., 84], respiration rate [e.g., 17], skin conductance [58], salivary cortisol [e.g., 54], salivary alpha-amylase (sAA) [e.g., 38], and salivary immunoglobulin-A (sIgA) [e.g., 85], as well as indicators for psychological consequences of the stress response, such as subjective stress [e.g., 18], perceived relaxation [e.g., 17], state anxiety [e.g., 21], rumination [e.g., 18], and affect [e.g., 37]. In our moderator analysis, we examined whether the effects of music listening on stress recovery differed across general (neuroendocrine, physiological, psychological) and specific outcome types.

Duration of music

Studies differed with regards to how long participants listened to music following stressor cessation. This duration ranged from two minutes [e.g., 53] to forty-five minutes [e.g., 54]. We examined whether the effect of music listening on stress recovery differed based on duration of music listening.

Data extraction, moderator coding, and quality assessment

KA extracted means, standard deviations, and total participants per condition for each stress recovery outcome. When these statistics were not included in text, but informative graphs were provided, KA used an open-source program to extract data from the graphs [86]. Coding criteria for each moderator can be found in Table 1. The ‘141–160 bpm’, ‘unsuccessful’, ‘salivary IgA’, and ‘salivary alpha-amylase’ moderator levels were ultimately not included in the meta-analysis due to unavailable information.

Table 1. Moderator coding criteria.

Moderator (bolded) and level Criteria
Classical vs. other genres
 Classical If no in-text description of genre was provided, the first author attempted to infer musical genre after listening to the reported musical stimuli. When this was also not possible, musical genre was coded as ‘Unspecified’.
 Heavy metal
 Jazz
 Pop
 Unspecified
Instrumental vs. lyrical
 Instrumental Music stimuli did not contain lyrics.
 Lyrical Music stimuli contained lyrics.
Self- vs. experimenter selected
 Self Music stimuli selected by participants.
 Experimenter Music stimuli selected by the experimenter(s).
 Pseudo Music stimuli selected by participants from an experimenter-defined list.
Fast vs. slow tempo
 80 bpm and below When no in-text description of tempo was provided, tempo values were retrieved using the Spotify Web API (https://developer.spotify.com) and rounded to the nearest integer.
 81–100 bpm
 101–120 bpm
 121–140 bpm
 141–160 bpm
 161 bpm and above
 Unspecified
Stress induction procedure
 With SET Stress induction procedure included a social-evaluative threat (SET) component.
 Without SET Stress induction procedure did not include a social-evaluative threat component.
Stress check
 Successful Stress induction procedure elicited an acute stress response.
 Unsuccessful Stress induction procedure did not elicit an acute stress response.
 Unreported Effect of stress induction procedure was not directly reported.
Outcome type (general)
 Neuroendocrine Includes cortisol & salivary IgA.
 Physiological Includes heart rate, heart rate variability indices, systolic and diastolic blood pressure, respiration rate, skin conductance, and salivary alpha-amylase.
 Psychological Includes subjective stress, perceived relaxation, state anxiety, state depression, rumination, positive affect, and negative affect.
Outcome type (specific)
 Cortisol
 Salivary IgA
 Heart rate
 Heart rate variability indices:
  RMSSD
  LF
  HF
  LF/HF
  Entropy
 Systolic blood pressure
 Diastolic blood pressure
 Respiration rate
 Skin conductance
 Salivary alpha-amylase
 Subjective stress
 Anxiety
 State depression
 Relaxation
 Rumination
 Positive affect
 Negative affect
Duration of music Kept as a continuous moderator.

Next, KA assessed the quality of included studies using the revised Cochrane risk of bias tool for randomized trials (RoB 2) [87]. Based on criteria in the RoB 2, studies with low risk of bias were considered high quality, while those with some concerns and high risk of bias were considered moderate and low quality respectively. Fig 2 summarizes the results of the quality assessment procedure.

Fig 2. Quality of included studies based on the RoB 2.

Fig 2

All studies were of overall moderate quality due to little-to-no information on pre-specification of analysis plans, making it difficult to fully rule out any bias that may occur due to selection of reported results.

Based on the RoB 2, all included studies were of moderate quality due to unavailable information on pre-specification of analysis plans. Thus, it was difficult to completely rule out bias that may have occurred due to a selection of reported results. Since the quality of included studies was homogenous, study quality was thus not included in our moderator analysis. An exploratory analysis with less stringent criteria, where potential risk of bias from selection of reported results is not included in our quality assessment procedure, is reported in Appendix B.

Data extraction, moderator coding, and quality assessment were conducted by KA in coordination with DB and MvH. Disagreements were resolved through face-to-face discussions, or through consultation with SG and KR when no consensus could be reached.

Meta-analytic approach

Effect size index

We calculated Hedges’ g for each comparison using the escalc function of the metafor package [88] in R 3.6.3 [89]. In the present study, a Hedges’ g of zero indicates the effect of music listening on stress recovery is equivalent to silence or an auditory control. Conversely, a Hedges’ g greater than zero indicates the degree to which music listening is more effective than control, while a g less than zero indicates the degree to which music listening is less effective than control. The effect sizes are reported in Table 2.

Table 2. Coded information and effect sizes of studies included in the meta-analysis (s = 14; k = 90).
Study Year Check SIP Selection Genre Lyrics Tempo Duration†† Outcome Type Outcome Measure N g
Chafin et al. 2004 Successful With SET Experimenter Classical Instrumental 101–120 10 Physiological Heart rate 30 0.47
Chafin et al. 2004 Successful With SET Experimenter Classical Instrumental 101–120 10 Physiological Systolic blood pressure 30 1.53
Chafin et al. 2004 Successful With SET Experimenter Classical Instrumental 101–120 10 Physiological Diastolic blood pressure 30 0.89
Chafin et al. 2004 Successful With SET Experimenter Jazz Instrumental > = 161 10 Physiological Heart rate 30 0.17
Chafin et al. 2004 Successful With SET Experimenter Jazz Instrumental > = 161 10 Physiological Systolic blood pressure 30 0.17
Chafin et al. 2004 Successful With SET Experimenter Jazz Instrumental > = 161 10 Physiological Diastolic blood pressure 30 0.07
Chafin et al. 2004 Successful With SET Experimenter Pop Lyrical 101–120 10 Physiological Heart rate 30 0.31
Chafin et al. 2004 Successful With SET Experimenter Pop Lyrical 101–120 10 Physiological Systolic blood pressure 30 0.41
Chafin et al. 2004 Successful With SET Experimenter Pop Lyrical 101–120 10 Physiological Diastolic blood pressure 30 0.23
Chafin et al. 2004 Successful With SET Pseudo Unspecified Unspecified Unspecified 10 Physiological Heart rate 30 0.35
Chafin et al. 2004 Successful With SET Pseudo Unspecified Unspecified Unspecified 10 Physiological Systolic blood pressure 30 0.36
Chafin et al. 2004 Successful With SET Pseudo Unspecified Unspecified Unspecified 10 Physiological Diastolic blood pressure 30 0.43
De la Torre-Luque et al. 2017a Successful With SET Self Unspecified Unspecified Unspecified 16 Physiological Heart rate 58 -0.16
De la Torre-Luque et al. 2017a Successful With SET Self Unspecified Unspecified Unspecified 16 Physiological RMSSD 58 -0.07
De la Torre-Luque et al. 2017a Successful With SET Self Unspecified Unspecified Unspecified 16 Physiological LF 58 0.44
De la Torre-Luque et al. 2017a Successful With SET Self Unspecified Unspecified Unspecified 16 Physiological HF 58 -1.04
De la Torre-Luque et al. 2017a Successful With SET Self Unspecified Unspecified Unspecified 16 Physiological LF/HF 58 -0.50
De la Torre-Luque et al. 2017a Successful With SET Self Unspecified Unspecified Unspecified 16 Physiological Entropy 58 0.57
De la Torre-Luque et al. 2017a Successful With SET Self Unspecified Unspecified Unspecified 16 Psychological Anxiety 58 0.57
De la Torre-Luque et al. 2017a Successful With SET Self Unspecified Unspecified Unspecified 16 Psychological State depression 58 0.61
De la Torre-Luque et al. 2017a Successful With SET Self Unspecified Unspecified Unspecified 16 Psychological Positive affect 58 0.45
De la Torre-Luque et al. 2017a Successful With SET Self Unspecified Unspecified Unspecified 16 Psychological Negative affect 58 0.66
De la Torre-Luque et al. 2017b Successful With SET Experimenter Unspecified Instrumental Unspecified 15 Physiological Entropy 21 -0.62
Fallon et al. 2020 Successful With SET Experimenter Unspecified Lyrical < = 80 5 Physiological Skin conductance 72 0.09
Gan, Lim, & Haw 2015 Unreported Without SET Experimenter Classical Instrumental 121–140 20 Physiological Heart rate 70 -0.17
Gan, Lim, & Haw 2015 Unreported Without SET Experimenter Classical Instrumental 121–140 20 Physiological Systolic blood pressure 70 0.17
Gan, Lim, & Haw 2015 Unreported Without SET Experimenter Classical Instrumental 121–140 20 Physiological Diastolic blood pressure 70 0.18
Gan, Lim, & Haw 2015 Unreported Without SET Experimenter Classical Instrumental > = 161 20 Physiological Heart rate 70 -0.26
Gan, Lim, & Haw 2015 Unreported Without SET Experimenter Classical Instrumental > = 161 20 Physiological Systolic blood pressure 70 -0.19
Gan, Lim, & Haw 2015 Unreported Without SET Experimenter Classical Instrumental > = 161 20 Physiological Diastolic blood pressure 70 0.04
Gan, Lim, & Haw 2015 Unreported Without SET Experimenter Classical Instrumental 121–140 20 Psychological Anxiety 70 0.60
Gan, Lim, & Haw 2015 Unreported Without SET Experimenter Classical Instrumental > = 161 20 Psychological Anxiety 70 0.06
Groarke & Hogan 2019 Successful With SET Self Unspecified Unspecified Unspecified 10 Psychological Subjective stress 80 1.19
Groarke & Hogan 2019 Successful With SET Self Unspecified Unspecified Unspecified 10 Psychological Anxiety 80 1.68
Groarke & Hogan 2019 Successful With SET Self Unspecified Unspecified Unspecified 10 Psychological Relaxation 80 1.51
Groarke & Hogan 2019 Successful With SET Self Unspecified Unspecified Unspecified 10 Psychological State depression 80 0.12
Groarke & Hogan 2019 Successful With SET Self Unspecified Unspecified Unspecified 10 Psychological Negative affect 80 0.52
Groarke et al. (study 1) 2020 Successful With SET Experimenter Unspecified Instrumental < = 80 8 Physiological Systolic blood pressure 46 0.28
Groarke et al. (study 1) 2020 Successful With SET Experimenter Unspecified Instrumental < = 80 8 Physiological Diastolic blood pressure 46 0.01
Groarke et al. (study 1) 2020 Successful With SET Experimenter Unspecified Instrumental < = 80 8 Psychological Anxiety 46 0.78
Groarke et al. (study 1) 2020 Successful With SET Self Unspecified Unspecified Unspecified 10 Physiological Systolic blood pressure 47 0.15
Groarke et al. (study 1) 2020 Successful With SET Self Unspecified Unspecified Unspecified 10 Physiological Diastolic blood pressure 47 -0.03
Groarke et al. (study 1) 2020 Successful With SET Self Unspecified Unspecified Unspecified 10 Psychological Anxiety 47 0.84
Groarke et al. (study 2) 2020 Successful With SET Experimenter Unspecified Instrumental < = 80 8 Physiological Systolic blood pressure 50 -0.17
Groarke et al. (study 2) 2020 Successful With SET Experimenter Unspecified Instrumental < = 80 8 Physiological Diastolic blood pressure 50 -0.08
Groarke et al. (study 2) 2020 Successful With SET Experimenter Unspecified Instrumental < = 80 8 Psychological Anxiety 50 -0.39
Groarke et al. (study 2) 2020 Successful With SET Self Unspecified Unspecified Unspecified 10 Physiological Systolic blood pressure 50 -0.22
Groarke et al. (study 2) 2020 Successful With SET Self Unspecified Unspecified Unspecified 10 Physiological Diastolic blood pressure 50 0.01
Groarke et al. (study 2) 2020 Successful With SET Self Unspecified Unspecified Unspecified 10 Psychological Anxiety 50 0.09
Khalfa et al. 2003 Successful With SET Self Unspecified Unspecified Unspecified 45 Neuroendocrine Cortisol 17 1.20
Koelsch et al. 2016 Successful Without SET Experimenter Unspecified Unspecified 101–120 41 Neuroendocrine Cortisol 143 -1.10
Labbé et al. 2007 Unreported Without SET Experimenter Classical Instrumental Unspecified 10 Physiological Heart rate 28 -0.01
Labbé et al. 2007 Unreported Without SET Experimenter Classical Instrumental Unspecified 10 Physiological Respiration rate 28 0.91
Labbé et al. 2007 Unreported Without SET Experimenter Classical Instrumental Unspecified 10 Physiological Skin conductance 28 -0.09
Labbé et al. 2007 Unreported Without SET Experimenter Heavy Metal Instrumental Unspecified 10 Physiological Heart rate 28 -0.12
Labbé et al. 2007 Unreported Without SET Experimenter Heavy Metal Instrumental Unspecified 10 Physiological Respiration rate 28 0.17
Labbé et al. 2007 Unreported Without SET Experimenter Heavy Metal Instrumental Unspecified 10 Physiological Skin conductance 28 -0.28
Gan, Lim, & Haw 2015 Unreported Without SET Experimenter Classical Instrumental > = 161 20 Physiological Systolic blood pressure 70 -0.19
Gan, Lim, & Haw 2015 Unreported Without SET Experimenter Classical Instrumental > = 161 20 Physiological Diastolic blood pressure 70 0.04
Gan, Lim, & Haw 2015 Unreported Without SET Experimenter Classical Instrumental 121–140 20 Psychological Anxiety 70 0.60
Gan, Lim, & Haw 2015 Unreported Without SET Experimenter Classical Instrumental > = 161 20 Psychological Anxiety 70 0.06
Groarke & Hogan 2019 Successful With SET Self Unspecified Unspecified Unspecified 10 Psychological Subjective stress 80 1.19
Groarke & Hogan 2019 Successful With SET Self Unspecified Unspecified Unspecified 10 Psychological Anxiety 80 1.68
Groarke & Hogan 2019 Successful With SET Self Unspecified Unspecified Unspecified 10 Psychological Relaxation 80 1.51
Groarke & Hogan 2019 Successful With SET Self Unspecified Unspecified Unspecified 10 Psychological State depression 80 0.12
Groarke & Hogan 2019 Successful With SET Self Unspecified Unspecified Unspecified 10 Psychological Negative affect 80 0.52
Groarke et al. (study 1) 2020 Successful With SET Experimenter Unspecified Instrumental < = 80 8 Physiological Systolic blood pressure 46 0.28
Groarke et al. (study 1) 2020 Successful With SET Experimenter Unspecified Instrumental < = 80 8 Physiological Diastolic blood pressure 46 0.01
Groarke et al. (study 1) 2020 Successful With SET Experimenter Unspecified Instrumental < = 80 8 Psychological Anxiety 46 0.78
Groarke et al. (study 1) 2020 Successful With SET Self Unspecified Unspecified Unspecified 10 Physiological Systolic blood pressure 47 0.15
Groarke et al. (study 1) 2020 Successful With SET Self Unspecified Unspecified Unspecified 10 Physiological Diastolic blood pressure 47 -0.03
Groarke et al. (study 1) 2020 Successful With SET Self Unspecified Unspecified Unspecified 10 Psychological Anxiety 47 0.84
Groarke et al. (study 2) 2020 Successful With SET Experimenter Unspecified Instrumental < = 80 8 Physiological Systolic blood pressure 50 -0.17
Groarke et al. (study 2) 2020 Successful With SET Experimenter Unspecified Instrumental < = 80 8 Physiological Diastolic blood pressure 50 -0.08
Groarke et al. (study 2) 2020 Successful With SET Experimenter Unspecified Instrumental < = 80 8 Psychological Anxiety 50 -0.39
Groarke et al. (study 2) 2020 Successful With SET Self Unspecified Unspecified Unspecified 10 Physiological Systolic blood pressure 50 -0.22
Groarke et al. (study 2) 2020 Successful With SET Self Unspecified Unspecified Unspecified 10 Physiological Diastolic blood pressure 50 0.01
Groarke et al. (study 2) 2020 Successful With SET Self Unspecified Unspecified Unspecified 10 Psychological Anxiety 50 0.09
Khalfa et al. 2003 Successful With SET Self Unspecified Unspecified Unspecified 45 Neuroendocrine Cortisol 17 1.20
Koelsch et al. 2016 Successful Without SET Experimenter Unspecified Unspecified 101–120 41 Neuroendocrine Cortisol 143 -1.10
Labbé et al. 2007 Unreported Without SET Experimenter Classical Instrumental Unspecified 10 Physiological Heart rate 28 -0.01
Labbé et al. 2007 Unreported Without SET Experimenter Classical Instrumental Unspecified 10 Physiological Respiration rate 28 0.91
Labbé et al. 2007 Unreported Without SET Experimenter Classical Instrumental Unspecified 10 Physiological Skin conductance 28 -0.09
Labbé et al. 2007 Unreported Without SET Experimenter Heavy Metal Instrumental Unspecified 10 Physiological Heart rate 28 -0.12
Labbé et al. 2007 Unreported Without SET Experimenter Heavy Metal Instrumental Unspecified 10 Physiological Respiration rate 28 0.17
Labbé et al. 2007 Unreported Without SET Experimenter Heavy Metal Instrumental Unspecified 10 Physiological Skin conductance 28 -0.28
Labbé et al. 2007 Unreported Without SET Self Unspecified Unspecified Unspecified 10 Physiological Heart rate 28 0.18
Labbé et al. 2007 Unreported Without SET Self Unspecified Unspecified Unspecified 10 Physiological Respiration rate 28 0.04
Labbé et al. 2007 Unreported Without SET Self Unspecified Unspecified Unspecified 10 Physiological Skin conductance 28 -0.12
Nakajima et al. 2016 Successful Without SET Experimenter Classical Instrumental 81–100 4 Physiological Heart rate 24 0.12
Nakajima et al. 2016 Successful Without SET Experimenter Classical Instrumental 81–100 4 Physiological LF 24 0.88
Nakajima et al. 2016 Successful Without SET Experimenter Classical Instrumental 81–100 4 Physiological HF 24 0.45
Nakajima et al. 2016 Successful Without SET Experimenter Classical Instrumental 81–100 4 Physiological LF/HF 24 0.24
Radstaak et al. 2014 Successful With SET Self Unspecified Unspecified Unspecified 5 Physiological Heart rate 60 0.18
Radstaak et al. 2014 Successful With SET Self Unspecified Unspecified Unspecified 5 Physiological Systolic blood pressure 60 -0.78
Radstaak et al. 2014 Successful With SET Self Unspecified Unspecified Unspecified 5 Physiological Diastolic blood pressure 60 -0.41
Radstaak et al. 2014 Successful With SET Self Unspecified Unspecified Unspecified 5 Physiological Heart rate 62 0.00
Radstaak et al. 2014 Successful With SET Self Unspecified Unspecified Unspecified 5 Physiological Systolic blood pressure 62 -0.51
Radstaak et al. 2014 Successful With SET Self Unspecified Unspecified Unspecified 5 Physiological Diastolic blood pressure 62 -4.18
Radstaak et al. 2014 Successful With SET Self Unspecified Unspecified Unspecified 5 Psychological Positive affect 63 0.67
Radstaak et al. 2014 Successful With SET Self Unspecified Unspecified Unspecified 5 Psychological Negative affect 63 0.12
Radstaak et al. 2014 Successful With SET Self Unspecified Unspecified Unspecified 5 Psychological Rumination 63 -0.45
Radstaak et al. 2014 Successful With SET Self Unspecified Unspecified Unspecified 5 Psychological Positive affect 65 0.96
Radstaak et al. 2014 Successful With SET Self Unspecified Unspecified Unspecified 5 Psychological Negative affect 65 -0.03
Radstaak et al. 2014 Successful With SET Self Unspecified Unspecified Unspecified 5 Psychological Rumination 65 -0.32
Scheufele 2000 Successful With SET Experimenter Classical Instrumental 81–100 15 Physiological Heart rate 33 2.48
Scheufele 2000 Successful With SET Experimenter Classical Instrumental 81–100 15 Psychological Relaxation 33 -0.49
Sokhadze 2007 Successful Without SET Experimenter Classical Instrumental 121–140 2 Physiological Heart rate 51 -0.65
Sokhadze 2007 Successful Without SET Experimenter Classical Instrumental 121–140 2 Physiological HF 51 0.26
Sokhadze 2007 Successful Without SET Experimenter Classical Instrumental 121–140 2 Physiological LF/HF 51 0.46
Sokhadze 2007 Successful Without SET Experimenter Classical Instrumental 121–140 2 Physiological Skin conductance 51 -0.43
Sokhadze 2007 Successful Without SET Experimenter Classical Instrumental 81–100 2 Physiological Heart rate 51 0.18
Sokhadze 2007 Successful Without SET Experimenter Classical Instrumental 81–100 2 Physiological HF 51 -0.18
Sokhadze 2007 Successful Without SET Experimenter Classical Instrumental 81–100 2 Physiological LF/HF 51 -0.27
Sokhadze 2007 Successful Without SET Experimenter Classical Instrumental 81–100 2 Physiological Skin conductance 51 0.67
Sokhadze 2007 Successful Without SET Experimenter Classical Instrumental 121–140 2 Psychological Anxiety 51 -0.11
Sokhadze 2007 Successful Without SET Experimenter Classical Instrumental 81–100 2 Psychological Subjective stress 51 0.06
Sokhadze 2007 Successful Without SET Experimenter Classical Instrumental 121–140 2 Psychological Anxiety 51 0.08
Sokhadze 2007 Successful Without SET Experimenter Classical Instrumental 81–100 2 Psychological Subjective stress 51 0.21

Note. A more detailed data file is available on the Open Science Framework.

= In beats per minute (BPM);

†† = in minutes.

Check = stress induction check/manipulation check; SIP = stress induction procedure; N = total observations for two group comparison; g = Hedges’ g.

Meta-analytic approach

Due to use of multiple stress recovery outcomes, eleven out of fourteen studies included in the meta-analysis contributed multiple effect sizes of interest. To deal with the statistical dependency caused by the inclusion of multiple effect sizes from the same study, we use a combination of multivariate meta-regression [90] and robust variance estimation (RVE) [91] to estimate overall effect sizes and conduct moderator analyses. Although we believe our approach using RVE was the most suitable for our data, we also calculated overall effect sizes using the aggregation method outlined in Borenstein et al. [92], and random-effects meta-analyses without correcting for dependencies. These yielded estimates that were nearly identical to those generated by our approach and were therefore not reported.

Outlier detection

Currently, methods to identify outliers in meta-regression models with RVE are not yet available. Therefore, we first fit a random-effects meta-regression model without correcting for dependencies between effect sizes. Values for influential case diagnostics (e.g., covariance ratios, Cook’s distance, studentized residuals) were subsequently requested using the ‘influence’ function of the ‘metafor’ package [88]. As this approach does not fully consider the nature of dependencies between effect sizes from each study, the results of this analysis were treated as a sensitivity analysis for the estimated overall effect of music listening on stress recovery. All extracted effect sizes were retained in further analyses.

Test of overall effect and moderators

To estimate the overall effect of music listening on stress recovery, we fit an intercept-only, random-effects meta-regression model with RVE using the ‘robu’ function of the ‘robumeta’ package [93]. The intercept estimated by this model can be interpreted as the precision-weighted overall effect size which has been corrected for dependencies. We used a similar approach to estimate cumulative effect sizes at each level of each moderator. For cases where a level of a moderator had too few observations for the RVE approach, we calculated cumulative effect sizes by fitting a random-effects meta-regression using the ‘rma.mv’ function of the ‘metafor’ package [88].

Prior to conducting moderator analyses, categorical moderators (e.g., ‘Genre’) were dummy coded, while the continuous moderator ‘Duration’ was left as is. For cases where the categorical moderator only had two levels, moderator variables were entered into separate meta-regression equations using the RVE approach. The significance test of the regression coefficient for the predictor variable in the meta-regression equation was interpreted as a test of whether the variable was a significant moderator. We used the same approach to test the effect of continuous moderators. For cases where the categorical moderator had more than two levels, moderator variables were entered into separate random-effects meta-regression models. This yielded QM and QE statistics: the QM statistic indicated whether there was a significant difference among all levels of the tested moderator, while the QE statistic indicated whether there were significant amounts of residual heterogeneity after accounting for the effect of the moderator [94].

Publication bias

The most common method to assess publication bias in meta-analytic data sets with dependent effect sizes is to aggregate individual effect sizes from the same study, and subsequently perform standard publication bias tests on the aggregated estimates. Therefore, we first aggregated individual effect sizes using the ‘agg’ function of the ‘MAd’ package [95]. The ‘agg’ function calculates aggregated effect size and variance estimates using formulas specified in Borenstein et al. [92]. These aggregated estimates were then used to assess publication bias by means of: (a) Egger’s regression of funnel plot asymmetry [96]; (b) a trim-and-fill analysis [97]; and (c) PET-PEESE models [98].

Results

Overall, the analyses comprised s = 14 studies, from which k = 90 effect sizes were calculated. The cumulative sample size of these studies was N = 706, while individual sample sizes ranged from 12–143 participants, with a mean of approximately 68 participants per study.

Overall effect

Based on a meta-regression with RVE, the estimated overall effect of music listening on stress recovery was g = 0.15, 95% CI [-0.21, 0.52], t(13) = 0.92, p = 0.374. This estimate suggests that, taking all variations in music and outcomes into consideration, the effect of music listening on stress recovery is equivalent to silence or an auditory control.

Outlier detection

Using the ‘influence’ function of the ‘metafor’ package [88], one influential outlier in the negative direction was detected [18]. The overall effect of music listening on stress recovery with outlier removed was g = 0.18, 95% CI [-0.18, 0.54], t(13) = 1.08, p = 0.300. The full meta-analytic data set was retained in subsequent analyses.

Moderator analyses

There was significant heterogeneity of effect sizes (T2 = 0.71, I2 = 89.29) from each study, which suggests that meaningful differences may exist among studies that could be further explored through moderator analyses. Cumulative effect size estimates at each level of each moderator, along with their respective significance tests, are reported in Table 3.

Table 3. Moderator analyses.

Moderator (bolded) and level s k g β 1 QM 95% CI p
Classical vs. other genres 14 90 - - 27.19 - < .001
 Classical 6 32 0.431 - - [-0.03, 0.88] 0.059
 Heavy metal 1 3 -0.076 - - [-0.64, 0.48] 0.619
 Jazz 1 3 0.137 - - [0.00, 0.27] 0.049
 Pop 1 3 0.317 - - [0.09, 0.53] 0.025
 Unspecified 10 49 0.067 - - [-0.42, 0.56] 0.765
Instrumental vs. lyrical 14 90 - - 3.44 - 0.179
 Instrumental 8 45 0.194 - - [-0.16, 0.55] 0.240
 Lyrical 2 4 0.159 - - [-1.13, 1.45] 0.362
 Unspecified 8 41 0.151 - - [-0.46, 0.78] 0.581
Self- vs. experimenter selected 14 90 - - 19.13 - < .001
 Self 6 37 0.336 - - [-0.29, 0.96] 0.226
 Experimenter 10 50 0.030 - - [-0.33, 0.45] 0.874
 Pseudo 1 3 0.377 - - [0.27, 0.48] 0.004
Fast vs. slow tempo 14 90 - - 43.66 - < .001
 80 bpm and below 2 7 0.084 - - [-0.06, 0.23] 0.086
 81–100 bpm 3 12 0.497 - - [-0.62, 1.62] 0.197
 101–120 bpm 2 7 -0.260 - - [-11.3, 10.8] 0.815
 121–140 bpm 2 10 0.067 - - [-1.58, 1.71] 0.696
 161 bpm and above 2 7 -0.020 - - [-1.33, 1.29] 0.870
 Unspecified 8 47 0.235 - - [-0.26, 0.73] 0.301
Stress induction procedure 14 90 - -0.450 - [-1.22, 0.32] 0.218
 With SET 9 56 0.319 - - [-0.15, 0.79] 0.154
 Without SET 5 34 -0.141 - - [-0.90, 0.62] 0.636
Stress check 14 90 - -0.108 - [-1.47, 1.26] 0.661
 Successful 12 73 0.173 - - [-0.26, 0.61] 0.399
 Unsuccessful 2 17 0.062 - - [-0.08, 0.20] 0.115
Outcome type (general) 14 90 - - 164.22 - < .001
 Neuroendocrine 2 2 -0.004 - - [-14.6, 14.6] 0.998
 Physiological 11 62 0.135 - - [-0.39, 0.67] 0.585
 Psychological 7 26 0.298 - - [-0.11, 0.71] 0.127
Outcome type (specific) 14 90 - - 374.12 - < .001
 Cortisol 2 2 -0.004 - - [-14.6, 14.6] 0.998
 Heart rate 8 16 0.236 - - [-0.40, 0.87] 0.412
 Heart rate variability indices:
  RMSSD 1 1 -0.069 - - [-0.58, 0.44] 0.794
  LF 2 2 0.562 - - [-1.96, 3.08] 0.216
  HF 3 4 -0.212 - - [-2.12, 1.69] 0.678
  LF/HF 3 4 -0.085 - - [-1.11, 0.934] 0.739
  Entropy 2 2 0.031 - - [-7.49, 7.55] 0.967
 Systolic blood pressure 4 12 -0.040 - - [-0.87, 0.74] 0.880
 Diastolic blood pressure 4 12 -0.442 - - [-2.39, 1.50] 0.522
 Respiration rate 1 3 0.362 - - [-0.79, 1.51] 0.309
 Skin conductance 3 6 0.038 - - [-0.29, 0.37] 0.659
 Subjective stress 2 3 0.665 - - [-6.02, 7.35] 0.426
 Anxiety 5 10 0.579 - - [-0.23, 1.39] 0.118
 State depression 2 2 0.345 - - [-2.79, 3.48] 0.395
 Relaxation 2 2 0.525 - - [-12.2, 13.3] 0.693
 Rumination 1 2 -0.383 - - [-1.17, 0.41] 0.102
 Positive affect 2 3 0.636 - - [-1.65, 2.92] 0.176
 Negative affect 3 4 0.404 - - [-0.39, 1.19] 0.155
Duration of music 14 90 - -0.005 - [-0.11, 0.10] 0.870

Note.

= moderator level contained too few observations to obtain an estimate using the RVE approach, so estimate was obtained by means of random-effects meta-regression.

s = number of studies; k = number of effect sizes; g = Hedges’ g. β1 coefficients are from separate meta-regressions with RVE, where a categorical moderator with two levels was dummy coded and entered into the model as a predictor; QM statistics are a Wald-type chi-square test which indicate whether there are significant differences among all levels of a moderator. The number of studies may not always add up, since most studies contributed multiple effect sizes.

Classical music vs. other genres

Our results suggest that the effect of music listening on stress recovery may differ across musical genres, QM(4) = 27.19, p < .001. Despite this, it is difficult to further elaborate on these differences as the individual estimated effects of pop (g = 0.317, 95% CI [0.09, 0.53], p = .025) and jazz music (g = 0.137, 95% CI [0.00, 0.27], p = .049) were derived from single studies, while the estimates for classical (g = 0.431, 95% CI [-0.03, 0.88], p = .059) and heavy metal music (g = -0.076, 95% CI [-0.64, 0.48], p = .619), along with music collapsed into the ‘unspecified’ category (g = 0.067, 95% CI [-0.42, 0.56], p = .765), were non-significant. Residual heterogeneity was statistically significant, QE(67) = 1147.43, p < .001.

Instrumental vs. lyrical

The effects of music listening on stress recovery did not differ between lyrical music (g = 0.159, 95% CI [-1.13, 1.45], p = .362), instrumental music (g = 0.194, 95% CI [-0.22, 0.65], p = .273), and music with ‘unspecified’ lyrical presence (g = 0.151, 95% CI [-0.46, 0.78], p = .581), QM(2) = 3.44, p = .179. Residual heterogeneity was statistically significant, QE(69) = 1171.95, p < .001.

Self- vs. experimenter selected

Our results suggest that there may be differences in magnitude between the effect of self-selected, pseudo self-selected, and experimenter selected music on stress recovery, QM(2) = 19.13, p < .001. However, these differences were difficult to expand on since the estimated effect of pseudo self-selected music (i.e., self-selected music from a list composed by experimenters) was derived from only one study (g = 0.377, 95% CI [0.27, 0.48], p = .004), while the estimated effects of self-selected (g = 0.336, 95% CI [-0.29, 0.96], p = .226) and experimenter selected music (g = 0.030, 95% CI [-0.33, 0.45], p = .874) were non-significant. Residual heterogeneity was statistically significant, QE(69) = 1139.39, p < .001.

Fast vs. slow tempo

Our results suggest that the effects of music listening on stress recovery may differ in magnitude based on musical tempo, QM(5) = 43.66, p < .001. However, little can be said about these differences since the estimated effects of music at 80 bpm or below (g = 0.084, 95% CI [-0.06, 0.23], p = .086), 81–100 bpm (g = 0.497, 95% CI [-0.62, 1.62], p = .197), 101–120 bpm (g = -0.260, 95% CI [-11.3, 10.8], p = .815), 121–140 bpm (g = 0.067, 95% CI [-1.58, 1.71], p = .696), 161 bpm and above (g = -0.020, 95% CI [-1.33, 1.29], p = .870), and ‘unspecified’ tempo (g = 0.235, 95% CI [-0.26, 0.73], p = .301) were non-significant. Residual heterogeneity was statistically significant, QE(67) = 1128.90, p < .001.

Stress induction procedure

There were no significant differences in the effects of music listening on stress recovery between studies whose stress induction procedures included SET (g = 0.319, 95% CI [-0.15, 0.79], p = .154) and those without SET (g = -0.141, 95% CI [-0.90, 0.62], p = .636), β1 = -0.450, p = .218.

Stress induction checks

There were no significant differences in the effects of music listening on stress recovery for studies with successful (g = 0.173, 95% CI [-0.26, 0.61], p = .399) and unreported (g = 0.062, 95% CI [-0.08, 0.20], p = .115) stress induction checks, β1 = -0.108, p = .661.

Type of outcome

Our results suggest that the effects of music listening on stress recovery may differ between neuroendocrine, physiological, and psychological outcomes QM(2) = 164.22, p < .001. These differences were challenging to further expand on since the estimated effects of music listening for neuroendocrine (g = -0.004, 95% CI [-14.6, 14.6], p = .794), physiological (g = 0.135, 95% CI [-0.39, 0.67], p = .585), and psychological (g = 0.298, 95% CI [-0.11, 0.71], p = .127) stress recovery outcomes were not statistically significant. We noted a similar pattern when comparing the effects of music listening between specific stress recovery outcomes: the magnitude of the effect of music listening may vary across stress recovery outcomes, QM(18) = 545.09, p < .001, but estimated effects per outcome were non-significant (Table 3). Residual heterogeneity was statistically significant despite the inclusion of general outcome type (QE(69) = 1018.57, p < .001) and specific outcome measure (QE(53) = 629.144, p < .001) as moderators.

Duration of music

There was no evidence that the effect of music listening on stress recovery may differ depending on how long participants were exposed to music, β1 = -0.005, p = .870 (rangeduration = 2–45 minutes).

To further illustrate the methodological heterogeneity among experimental studies on the effect of music listening on stress recovery, we provide a more extensive, qualitative overview of the included studies in Appendix C. A summary of this overview is presented in Table 4.

Table 4. Summary of studies included in the systematic review.
No. Authors (Year) N Stress induction procedure Music stimulus (Song [tempo]) Measured outcomes Reported findings
1 Chafin et al. (2004) 75 Arithmetic
Description: mental arithmetic with harassment.
Participants were asked to count back from a large, random number in odd steps (e.g., “Count backwards from 9000 in steps of 17”) while being repeatedly interrupted (harassed) by the experimenter at timed intervals (e.g., “You are too slow, start over”).
Duration: 5 minutes
Classical
(Pachelbel–Canon in D major, [130 bpm]; Vivaldi–The Four Seasons: Spring, Movement I, [90 bpm])
Jazz
(Miles Davis–Flamenco Sketches, [177 bpm])
Top 40 Pop
(Sarah McLahlan–Angel [117 bpm]; Dave Matthews Band–Crash Into Me, [101 bpm])
Self-selected
(Unspecified)
Duration: 10 minutes
Physiological
Systolic blood pressure
Diastolic blood pressure
Heart rate
Psychological
Anxiety (STAI, form A)
Rumination (1–7 scale)
Relaxation (1–7 scale)
Significant effect of music on systolic blood pressure, with classical music returning systolic blood pressure closer to baseline compared to control condition (+).
Similar pattern as systolic blood pressure, but not significant (-).
No significant differences between groups (-).
No significant differences between groups (-).
No significant differences between groups (-).
No significant differences between groups (-).
2 de la Torre-Luque et al. (2017a) 21 Modified Trier Social Stress Task (TSST)
Description: modified TSST with PASAT.
Participants were asked to deliver a presentation in front of a camera, with the video feed and a timer displayed on a nearby laptop. The mental arithmetic component was substituted with the Paced Auditory Serial-Addition Task (PASAT). In the PASAT, participants are presented with a number every three seconds, and are asked to add the current presented number with the number presented before (Gronwall, 1977).
Duration: 15 minutes
Unspecified
(Melomics relaxing music)
Duration: 16 minutes
Physiological
Heart rate variability
(HR, RMSSD, LF, HF, LF/HF, SampEn)
Psychological
Anxiety (STAI)
Significant differences in HR, LF, HF, and SampEn at baseline and recovery phases. Music group demonstrated significantly higher SampEn during recovery phase compared to control group (+).
Significant difference in anxiety across study phases, but not between groups (-).
3 de la Torre-Luque et al. (2017b) 58 Modified TSST
Description: modified TSST with PASAT.
Duration: 15 minutes
Self-selected
(Unspecified)
Duration: 15 minutes
Physiological
Heart rate variability
(HR, RMSSD, LF, HF, LF/HF, SampEn)
Psychological
Anxiety (STAI)
Depression (ST-DEP)
Positive affect (PANAS)
Negative affect (PANAS)
Significant differences in HR, LF, LF/HF, and SampEn across study phases. Music group demonstrated significantly higher HF and SampEn during recovery phase compared to control group (+).
Anxiety scores for music group during recovery phase significantly lower compared to control group (+).
Depression scores for music group during recovery phase significantly lower compared to control group (+).
Positive affect for music group during recovery phase significantly higher compared to control group (+).
Negative affect for music group during recovery phase significantly lower compared to control group (+).
4 Fallon et al. (2020) 105 Modified TSST
Description: standard TSST with shorter mental arithmetic component.
Duration: 11 minutes
Unspecified
(Eric Whitacre–Sleep [50 bpm])
Duration: 5 minutes
Physiological
Skin conductance
Psychological
Current mood (Irritated, Satisfied, Excited, Distracted, Tingling feeling, Calm)
Significant differences in skin conductance between study sessions (baseline, stressor, recovery). Significant differences in skin conductance between music listening group and silence control group during recovery session (+)
Significant differences in current mood between study sessions. Music listening intervention did not have differential effects on current mood compared to control group (-)
5 Gan et al. (2016) 105 Arithmetic
Description: participants were asked to complete 12 questions from the University of Cambridge General Certificate of Education (GCE) Ordinary-level mathematics examinations.
Duration: 15 minutes
Stimulative
(Beethoven–Moonlight Sonata No.14 in C-sharp Minor Op. 72 No. 2, [171 bpm])
Sedative
(Camille Saints-Saens–Allegro Moderato, Symphony No. 3 Op. 78, Movement III, [121 bpm])
Duration: approx. 20 minutes
Physiological
Systolic blood pressure
Diastolic blood pressure
Heart rate
Psychological
Anxiety (STAI, form X-1)
Math anxiety (MARS)
No significant effect of music post-stressor. Significant difference in systolic blood pressure post-music compared to baseline for stimulative music, sedative music, and control groups (-).
No significant effect of music post-stressor. Significant difference in diastolic blood pressure post-music compared to baseline for sedative music and control groups (-).
No significant differences between groups (-).
Significant decrease in post-stress anxiety scores for sedative music group compared to control group (+).
Significant decrease in post-stress mathematics anxiety scores for sedative music group compared to control group (+).
6 Groarke & Hogan (2019) 40 Modified TSST
Description: standard TSST with speech component omitted.
Duration: 10 minutes
Self-selected
(Unspecified)
Duration: 10 minutes
Psychological
Subjective stress
Nervousness
Tension
Upset
Sadness
Depressed affect
Significant differences in subjective stress between music group and control group (+).
Significant differences in nervousness between music group and control group (+).
No significant differences in tension between study groups (-).
Significant differences in upset regulation between music group and control group (+).
Significant differences in sadness between music group and control group (+).
Significant differences in depressed affect between music group and control group (+).
7 Groarke et al. (2020) 70 Modified TSST
Description: standard TSST with speech component omitted.
Duration: 8 minutes
Unspecified
(Marconi Union–Weightless [71 bpm]
Self-selected
(Unspecified)
Duration: approx. 10 minutes
Physiological
Systolic blood pressure
Diastolic blood pressure
Physiological
Anxiety (STAI)
Significant changes in systolic blood pressure across study phases, but no significant differences in systolic blood pressure recovery between study conditions (-).
Significant changes in diastolic blood pressure across study phases, and no significant differences in diastolic blood pressure recovery between study conditions (-).
Significant differences in post-stressor anxiety for both music groups compared to control group (+).
75 Modified TSST
Description: standard TSST with speech component omitted.
Duration: 8 minutes
Unspecified
(Marconi Union–Weightless [71 bpm]
Self-selected
(Unspecified)
Duration: approx. 10 minutes
Physiological
Systolic blood pressure
Diastolic blood pressure
Physiological
Anxiety (STAI)
Significant changes in systolic blood pressure across study phases, but no significant differences in systolic blood pressure recovery between study conditions (-).
No significant changes in diastolic blood pressure across study phases, and no significant differences in diastolic blood pressure recovery between study conditions (-).
No significant differences in post-stressor anxiety between all study groups (-).
8 Khalfa et al. (2003) 17 TSST
Description: standard TSST.
Participants were asked to deliver an impromptu, interview-style presentation in front of a panel of judges who do not provide feedback or encouragement. This presentation is followed by a surprise mental arithmetic task (Allen et al., 2017; Kirschbaum, Pirke, & Hellhammer, 1993).
Duration: 15 minutes
Unspecified
(Various songs by Enya, Vangelis, & Yanni)
Duration: 45 minutes
Physiological
Cortisol
Significant, rapid decrease in post-stressor cortisol in music group compared to control group (+).
9 Koelsch et al. (2016) 143 CO2 Stress Task
Description: participants were instructed to take a single, vital-capacity breath of air containing 35% carbon dioxide and 65% oxygen. The CO2 Stress Task is known to provoke panic attacks in many individuals with panic disorder, and has recently been used in stress research as an acute physiological stressor (Vickers, Jafarpour, Mofidi, Rafat, & Woznica, 2012).
Duration: n/a
Unspecified
(Unspecified)
Duration: approx. 41 minutes
Physiological
Cortisol
Psychological
Mood (POMS, measures Depression/Anxiety, Fatigue, Vigor, Irritability)
Increase in post-stressor cortisol for music group significantly higher compared to control group (-).
Significant increase in post-stressor positive mood scores in music group compared to control group (+).
10 Labbé et al. (2007) 56 Arithmetic
Description: mental arithmetic operations were part of a broader “cognitive speed test” which also included number memory items, verbal analogy items, and spelling items.
Duration: 10 minutes
Classical
(Unspecified)
Heavy metal
(Unspecified)
Self-selected
(Unspecified)
Duration: 20 minutes
Physiological
Heart rate
Respiration rate
Skin conductance
Psychological
Relaxation (RSS)
Anxiety (STAI, form Y)
No significant differences between groups (-).
No significant differences between groups (-).
Post-hoc, all groups experienced significant post-stressor decrease in skin conductance, which was larger for the classical and self-selected music groups (+).
Relaxation scores for classical, self-selected, and silence groups significantly higher post-stressor compared to heavy metal group (-).
Anxiety scores for classical and self-selected music groups significantly lower post-stressor compared to heavy metal and silence groups (+).
11 Nakajima et al. (2016) 12 Unpleasant stimuli
Description: friction noise made by scratching a blackboard.
Duration: 90 seconds
Classical
(Mozart–Horn Concerto No.2 in E flat major, Movement II, [111 bpm])
Duration: approx. 4 minutes
Physiological
Heart rate variability
(HR, HFnu, LFnu, LF/HF)
HFnu significantly higher for music stimulus with amplified high frequency component, compared to music stimulus with amplified low frequency component (+).
12 Radstaak et al. (2014) 123 Arithmetic
Description: mental arithmetic task with harassment.
Duration: 5 minutes
Relaxing
(Unspecified)
Happy
(Unspecified)
Duration: 5 minutes
Physiological
Systolic blood pressure
Diastolic blood pressure
Heart rate
Psychological
Positive affect (1–10 scale)
Negative affect (1–10 scale)
Rumination (1–10 scale)
Systolic blood pressure during recovery phase for relaxing music and happy music groups significantly higher compared to audio and silence control groups (-).
No significant differences between groups (-).
No significant differences between groups (-).
Significant increase in positive affect during recovery phase for relaxing music and happy music groups compared to audio and silence control groups (+).
No significant differences between groups (-).
No significant differences between groups (-).
13 Scheufele (2000) 67 Anticipation
Description: faux presentation.
Duration: 15 minutes
Classical
(Mozart–Sonata in D major for Two Pianos, [100 bpm]])
Duration: 15 minutes
Physiological
Heart rate
Psychological
Relaxation (VAS, very tense-very relaxed)
Mood (POMS-SF, Tension subscale)
Significant differences in heart rate post-stressor for music group compared to attention control group (+).
No significant differences between groups (-).
No significant differences between groups (-).
14 Sokhadze (2007) 29 Unpleasant stimuli
Description: nine pictures from the International Affective Picture System (IAPS; Lang, Bradley, & Cuthbert, 1997), which were presented to participants in series of three pictures. The nine IAPS pictures used in the study had been previously rated as strongly eliciting disgust (e.g., a mutilated body).
Duration: 20 seconds per picture
Pleasant
(Spring Song, [82 bpm])
Sad
(Pachelbel–Canon in D major, [130 bpm])
Duration: 2 minutes
Physiological
Electrodermal activity
(SCL, SCR-M, NS.SCR)
Heart rate variability
(HR, LF, HF, LF/HF)
Psychological
Anxiety (1–7 scale)
Depression (1–7 scale)
Subjective stress (1–7 scale)
NS.SCR for pleasant music group significantly lower during music compared to during stressor (+).
HF for pleasant music group significantly lower during music compared to during stressor. Post-stressor HF was significantly lower for pleasant music group compared to control group (-).
No significant differences between groups (-).
No significant differences between groups (-).
No significant differences between groups (-).

Note. (+) = Finding in support of the effect of music on physiological recovery from stress; (-) = Finding not in support of the effect of music on physiological recovery from stress.

Publication bias

To visually assess the extent of publication bias, the aggregated effect size estimates in our meta-analytic data set were first used to create a plot of the estimates and their standard errors. In the absence of publication bias, this pattern should resemble a funnel, where effect size estimates with smaller standard errors cluster around the mean effect size, while effect size estimates with larger standard errors spread out in both directions. A common pattern which suggests publication bias is asymmetry in the bottom of the plot. Fig 3 presents the funnel plot of the aggregated effect sizes.

Fig 3. Funnel plot of studies examining the effect of music listening on psychophysiological recovery from stress.

Fig 3

The small number of studies renders it difficult to visually inspect asymmetry, and thus precludes an accurate assessment of publication bias.

Given the limited number of studies included in the meta-analysis (n = 14), an accurate visual assessment of asymmetry was difficult. Thus, to supplement our visual inspection of the funnel plot, we conducted a trim-and-fill analysis, which trims the values of extreme estimates that may lead to asymmetry in the funnel plot and imputes values to balance out the distribution. No studies were imputed by the trim-and-fill analysis. Additionally, an Egger’s regression for funnel plot asymmetry using the aggregated effect sizes failed to detect significant evidence of publication bias (t(12) = 1.26, p = 0.231). Lastly, both PET (β1 = 2.63, p = 0.311) and PEESE (β1 = 3.87, p = 0.356) models were not statistically significant. Taken together, based on the aggregated effect sizes, the different methods of publication bias detection suggest that there is no evidence of publication bias. However, considering the small number of included studies and the significant heterogeneity of our meta-analytic data set, firm conclusions about the extent of publication bias within the current literature on the effects of music listening and stress recovery are difficult to make.

Discussion

Music listening has the potential to fulfill the promise of effective stress recovery in healthy individuals. However, cumulative evidence from 17 experimental studies suggests that support for the beneficial effect of music listening on stress recovery is currently lacking: for healthy individuals, the effect of music listening on stress recovery may be equivalent to that of other auditory stimuli, or even merely sitting in silence. Furthermore, the effect of music listening on stress recovery is heterogeneous, and moderator analyses suggest the effect may differ in magnitude according to musical genre, whether music is self-selected, musical tempo, and type of stress recovery outcome. Despite this, the limited number of available studies makes it difficult to draw further conclusions from these analyses.

Overall effects of music listening on stress recovery

The results of our review contrast those of previous meta-analyses, which underscore the relevance of music-based interventions for stress-reduction [10, 11]. While previous reviews suggest that music-based interventions may be moderately beneficial for stress-related outcomes, particularly in medical and therapeutic settings, our results suggest that the magnitude of this effect outside of these settings, particularly for healthy individuals under acute, experimentally induced stress, may be more modest. We presume that one of the principal reasons for this difference was our decision to exclude studies conducted in medical and therapeutic settings. In previous reviews, randomized controlled trials of the effects of music-based interventions within medical and therapeutic settings constituted a large portion of included studies: 67 of 79 (85%) studies in de Witte et al. [10], and 15 of 22 (68%) studies in Pelletier [11], making it more likely that overall effect sizes were derived from studies conducted within these settings. Tentatively, the effects of music listening may be more prominent for the stress recovery of individuals in medical or therapeutic contexts, compared to that of individuals under acute stress in an experimental context. Whereas the time course of stress responses and stress recovery in experimental settings can be considered relatively brief [24, 26, 40, 83], the time course of stress responses and stress recovery within medical and therapeutic settings may be significantly more protracted [12, 13]. Thus, within medical and therapeutic settings, music may be exerting its influence on neuroendocrine, physiological, and psychological processes that have been subjected to longer periods of strain [27, 99].

Furthermore, the difference in overall estimated effect sizes may be attributed to differences in the breadth of music activities encompassed by our review and that of de Witte et al. [10]: whereas we included studies in which participants merely listened to music following a stressor, de Witte et al. [10] also included music therapy, along with other unspecified music activities. We speculate that the effect of music on stress recovery may differ depending on whether music is merely listened to, performed, or used within a music therapy setting. However, studies comparing the stress recovery effects of these various music activities are rare [15, 58]. Thus, future investigations into the differential effect of these music activities may therefore provide a more comprehensive picture of the effects of music on stress recovery.

Potential moderating effects

Our review highlights the considerable methodological variety between studies investigating the effects of music listening on stress recovery. This is particularly concerning given the modest number of experimental studies on music listening and stress recovery in current literature. Although we investigated the impact of these methodological differences through moderator analyses, many of the estimated effects at each level of each moderator were either non-significant or originated from single studies. Taken together, meaningful interpretations for these moderating effects are difficult to make. Therefore, for each significant moderator, we instead provide several recommendations for future research, which we believe may help delineate the effects of these potential moderators.

Musical genre

Although comparisons between musical genres seem relatively straightforward, investigating the differential effects of musical genres may be particularly challenging: the conceptualization of musical genres, along with the songs they encompass, tends to be somewhat arbitrary [69, 75, 100, 101]. Indeed, studies display considerable variation in musical stimuli, even within the same genre (Table 4). A notable example of this is the study by Sandstrom and Russo [53], which utilized four ‘classical’ songs, each at different extremes of valence and arousal. It should also be considered that new music is continuously being released which may not completely fit with the definition of any existing genre [9].

As such, an alternative approach to the investigation of musical genre involves describing these genres according to their musical features, such as tempo, timbre, and loudness, and subsequently investigating the effects of these individual musical features on stress recovery [9, 101]. For example, classical music may be described as rhythmically complex, with mellow timbre and fluctuating loudness. Comparatively, though equally rhythmically complex, heavy metal possesses sharper timbre and more pronounced loudness. Investigating the differential effects of these musical features on stress recovery may provide relevant insight into the differential effects of listening to various musical genres on stress recovery.

Self- versus experimenter selection

In investigating the effects of self- versus experimenter selected music on stress recovery in healthy individuals, studies typically request participants to select music they consider ‘relaxing’ prior to an experiment [3, 17, 18]. Although this approach is viable, it precludes the potential role of perceived control in the relationship between music listening and stress recovery, since allowing participants to self-select their own music may already be helpful for stress recovery due to a restoration of perceived control [15]. Our results were not able to provide a significant contribution to this discussion, as hardly any experimental studies in our review have attempted to account for the potential effects of perceived control. As such, when contrasting the effects of self- and experimenter selected music on stress recovery, future studies may benefit from the inclusion of perceived control as an additional variable in their theoretical models.

It should also be noted that allowing participants to self-select their own music will result in a considerable variety of musical stimuli. Given that each of these musical stimuli may possess a different combination of musical features, the use of self-selected music may generate confounding effects that should preferably be accounted for. Arguably, self-selected music may produce consistent effects on stress-recovery regardless of underlying musical features, given that individuals tend to select music in service of personal self-regulatory goals [64, 75, 76]. However, given that variations in specific musical features, such as tempo, pitch, and loudness have been related to various physiological (e.g., heart rate) [73] and psychological stress recovery outcomes (e.g., positive and negative affect) [100102], future studies may benefit from ensuring that musical features are consistent between self- and experimenter selected musical stimuli. This may be done, for instance, by comparing expert ratings of musical features [18]. Alternatively, there may be value in allowing participants to self-select music from a list provided by experimenters [21], as this would allow experimenters to standardize musical features a-priori, which may further help disentangle the effects of music listening from that of perceived control.

The comparison of musical features between self-selected and experimenter selected music may also offer a more nuanced perspective on the role of preference and familiarity. Specifically, preferences and familiarity towards certain songs could be described in terms of specific (combinations of) musical features. For example, an individual may prefer music with slow tempo, mellow timbre, and moderate loudness. This approach is often leveraged by music recommender systems, such as those implemented by music streaming platforms (e.g., Spotify, Deezer, Apple Music, etc.), with the goal of recommending songs that listeners are likely to engage with. Future studies could investigate the extent to which preference and familiarity might differ between self-selected and experimenter selected music with similar combinations of musical features, to further clarify the role of selection in the relationship between music listening and stress recovery.

Musical tempo

The systematic review portion of our results demonstrates that no studies have directly compared the effect of different musical tempi on stress recovery in healthy individuals. As such, the most straightforward approach to delineate the effects of musical tempo on stress recovery would be to adopt procedures in which participants listen to the same musical stimulus post-stressor, which is then varied in tempo across experimental conditions. Furthermore, even when the goal of a particular study on music listening and stress recovery is not to clarify the effects of musical tempo, we suggest that tempo values for each musical stimulus should be noted down and reported, as this would facilitate the comparison of the differential effects of musical tempo on stress recovery in future meta-synthesis of the literature.

Alternatively, the notion that music with slow tempo is more beneficial for stress recovery compared to music with fast tempo is supported by the assumption that physiological parameters will entrain to musical rhythms [63, 68]. As such, a more accurate approach to investigate the effects of musical tempo on stress recovery would be to leverage the dynamic, temporal nature of both music and physiological parameters through use of non-linear analyses of continuous data [52, 103]. For example, cross-recurrence quantification analysis (CRQA) [104, 105] may enable future studies to quantify the magnitude and duration of rhythmic entrainment for each participant. These indexes of magnitude and duration could then be compared between different musical tempi. Studies have utilized CRQA to investigate cardiac entrainment between participants of collective rituals [106] and the entrainment of an audience’s heart rate to a live musical performance [107]. This analytical approach may therefore yield a more nuanced understanding of the effect of musical tempo on the recovery of autonomic parameters.

Stress recovery outcomes

During short-term stress responses, catecholamine- and cortisol-mediated stress responses follow temporally specific patterns: catecholamines rapidly exert their influence on ANS activity, and these changes tend to normalize within 30–60 minutes [26]. Meanwhile, decreases in cortisol that may be attributed to stress recovery will only become noticeable after recovery-related changes in autonomic activity have begun to occur [24]. As such, to further clarify the effect of music listening on various stress recovery outcomes, we recommend future studies to be more sensitive towards the innate, intricate, and temporally specific changes of each stress recovery outcome.

Furthermore, multiple studies included in our review have opted to analyze continuous data by means of multivariate analyses of variance, after averaging participants’ observed stress recovery outcomes at multiple time points (e.g., pre-stress, post-stress, post-recovery). Although this approach is practical, doing so may over-simplify the complex changes that may occur during the stress response and subsequent stress recovery, such as the temporal dynamics of different physiological responses [52] and emotion regulation strategies [108]. As such, we again suggest future studies to utilize non-linear analyses of data when appropriate, particularly when investigating the effects of music listening on the recovery of autonomic activity post-stressor. The idea of using non-linear analyses, such as time-series analysis, to investigate the stress recovery process is not new [5]. However, few studies on music listening and stress recovery have utilized this analytical approach.

Additional recommendations

Two studies with unreported stress induction procedures were still included in the review [17, 84], as reported means for certain recovery outcomes still suggested an increase from baseline that participants could recover from. For example, with the information reported in Gan et al. [84], assuming a correlation of 0.5 between baseline and post-stressor measures of state anxiety, we estimated that their stress induction procedure elicited a significant increase in state anxiety in their sedative music (t(34) = 5.87, p < .001, mdiff = 8.17, SDdiff = 8.24), stimulative music (t(34) = 8.21, p < .001, mdiff = 12.42, SDdiff = 8.95), and control (t(34) = 13.15, p < .001, mdiff = 15.83, SDdiff = 7.12) conditions. As the overall estimated effect of music listening on the recovery process of healthy individuals following laboratory stressors may be relatively modest, it becomes particularly important to ensure that a sufficient stress response is elicited, to provide a larger window of opportunity in which the effect of music listening may be exerted on participants’ recovery processes. We thus encourage future studies to adopt validated, (variations of) well-known stress tasks, such as the TSST [109], SECPT [110], or CO2 stress task [111], which have been demonstrated to consistently elicit marked physiological and psychological stress-related responses in laboratory settings. Furthermore, we remind future studies to candidly report the results of their stress induction procedures to facilitate subsequent meta-syntheses of the effects of music listening on stress recovery.

As the current review focused on the effects of music listening after a stressor, studies where music was played before or during a stressor were omitted from our analyses. However, several studies suggest that the timing at which music is played (i.e., before, during, or after a stressor) may influence its effects on stress recovery. For example, in Burns et al. [48], participants who listened to classical music while anticipating a stressful task exhibited lower post-music heart rate compared to participants who anticipated the stressor in silence. Similarly, concentrations of salivary cortisol were lower for participants who watched a stressful visual stimulus while listening to music compared to those who watched the same stimulus without music [112]. Together, these findings hint that, when listened during a stressor, music may attenuate cortisol responses [9, 113], thus reducing the subsequent need for recovery. On the other hand, Thoma et al. [9] reported that participants who listened to music prior to a stressor exhibited higher post-stressor cortisol compared to participants who listened to an audio control. Interestingly, despite the stronger stress response, Thoma et al. [9] noted a trend for quicker ANS recovery among participants who listened to music, particularly with regards to salivary alpha-amylase activity. This pattern of findings is consistent with the notion forwarded by Koelsch et al. [61], in that music listening may promote a more adaptive stress response, thus facilitating subsequent stress recovery processes. To date, research on timing differences in the context of music listening and stress recovery is scarce. Thus, future studies could further examine the influence of such timing differences to better understand their role in the relationship between music listening and stress recovery.

Given the pervasiveness of stress, Ecological Momentary Assessment (EMA) studies may provide a more intimate outlook on the dynamics of daily music listening behaviour, particularly for the purpose of stress recovery. For example, through an ambulatory assessment study, Linnemann et al. [38] revealed that music produced the most notable reductions in physiological and psychological stress outcomes when it was listened to for the purpose of ‘relaxation’, compared to other reasons such as ‘distraction’, ‘activation’, and ‘reducing boredom’. Indeed, given their high ecological validity, EMA studies may provide further insight into important contextual variables in the relationship between music listening and stress recovery. For example, in an EMA study, listening to music in the presence of others was related to decreased subjective stress, attenuated cortisol secretion, and higher activity of salivary alpha-amylase [55]. Furthermore, physiological responses to music may co-vary between members of a dyad when music is listened to by couples [114]. Thus, given the benefits of EMA studies, we invite future studies to continue exploring the dynamics and contextual factors of music listening behaviour for stress recovery in daily life.

Lastly, we encourage studies to support open science research practices, and to clearly report statistical information that may be relevant for meta-syntheses (e.g., means and standard deviations per time point, per experimental condition, etc.). Additionally, based on our assessment of study quality using the RoB 2, pre-registration of analysis plans can be helpful to ensure that the conducted study is of overall high quality. Next, we encourage studies to note down which specific musical stimuli were used, particularly those self-selected by participants [69, 99], as this enables future exploratory analyses of structural commonalities between different musical stimuli. Musical features from individual songs may be extracted by means of audio information extraction packages, such as MIRtoolbox [115]. Alternatively, individual song titles may be used to query related meta-data from online databases of various music streaming platforms. This meta-data can subsequently be used to obtain additional insight into the effects of music listening on stress recovery.

Limitations of the current review

To our knowledge, our review is the first to comprehensively investigate the effect of music listening on stress recovery within healthy individuals. Given the explicit focus of our review, our meta-analytic data set excluded the more prominent effects of music listening in both medical and therapeutic settings [12, 13], allowing us to obtain results that are tentatively more representative of daily stress recovery processes. Despite this, the present review is not without its limitations:

First, although the specific focus of our review has allowed us to obtain a portrait of the effects of music listening on stress recovery in well-controlled experimental settings, the results of our review may be difficult to generalize to situations in which individuals experience prolonged stress responses. Stress induction procedures in experimental studies are designed to elicit acute stress responses that are meant to subside upon conclusion of an experiment [83]. Although we believe these procedures provide a suitable approximation of typical stressors in daily life, certain stressors in daily life may also persist for a longer time. The manner and magnitude in which music listening influences prolonged stress responses may potentially differ from the way music influences acute, laboratory-induced stress responses [18, 45]. However, studies investigating the effect of music listening on stress recovery in the long-term are particularly rare.

Next, despite our best efforts to obtain relevant meta-analytic information from all studies selected for our review, our meta-analytic data set was ultimately constructed from a subset of fourteen studies. Although the subset allowed us to extract sufficient information to estimate an overall effect of music listening on stress recovery, several estimated effects at moderator level were derived from merely one or two studies (see Table 3). This precluded us from drawing further, meaningful conclusions about the results of our moderator analyses.

Finally, despite our clear focus on the effects of music listening on stress recovery within healthy individuals, there was substantial heterogeneity in our meta-analytic data set that could not be fully explained by the inclusion of moderators. Although the systematic review portion of our results highlighted potential additional sources of between-study heterogeneity, these additional sources could not be evaluated in our meta-analytic data set. We note, for example, that all studies utilized different musical stimuli to investigate the effect of music listening on stress recovery (see Table 4). The differential effects of these musical stimuli were difficult to account for in our meta-analysis, given the limited number of included studies. Overall, the significant heterogeneity in our meta-analytic data set suggests that our moderator analyses should be interpreted with caution.

Conclusion

Studies commonly suggest that listening to music may have a positive influence on stress recovery. Based on cumulative evidence from 90 effect sizes in 14 studies, it may be premature to firmly conclude whether music listening is beneficial for the stress recovery of healthy individuals. The present review underscores the necessity for further and finer research into the effects of music, bearing the potential role of various moderators, such as musical genre, self-selection, musical tempo, and different stress recovery outcomes, to fully comprehend the nuanced effects of music listening on short-term stress recovery.

Appendix A

Search strategy

Using the advanced search feature within RUQuest, Web of Science, and PsycINFO, the following syntax was used so that the search returned results if keywords were found within the title, abstract, or keywords of relevant publications:

ti: (music* OR “music listening”) AND ((stress* OR strain OR recover* OR relax* OR fatigue OR “heart rate” OR “heart rate variability” OR “blood pressure” OR cardiovascular OR physiological OR cortisol OR “perseverative cognition” OR ruminat* OR detachment OR distract* OR worry* OR emotion* OR affect* OR mood OR burnout OR depress*) NOT (patient OR disease OR surgery OR operating OR theat?? OR disorder OR clinical OR stroke OR animal OR dent* OR material OR recogni* OR recommend*))

OR

ab: (music* OR “music listening”) AND ((stress* OR strain OR recover* OR relax* OR fatigue OR “heart rate” OR “heart rate variability” OR “blood pressure” OR cardiovascular OR physiological OR cortisol OR “perseverative cognition” OR ruminat* OR detachment OR distract* OR worry* OR emotion* OR affect* OR mood OR burnout OR depress*) NOT (patient OR disease OR surgery OR operating OR theat?? OR disorder OR clinical OR stroke OR animal OR dent* OR material OR recogni* OR recommend*))

OR

kw: (music* OR “music listening”) AND ((stress* OR strain OR recover* OR relax* OR fatigue OR “heart rate” OR “heart rate variability” OR “blood pressure” OR cardiovascular OR physiological OR cortisol OR “perseverative cognition” OR ruminat* OR detachment OR distract* OR worry* OR emotion* OR affect* OR mood OR burnout OR depress*) NOT (patient OR disease OR surgery OR operating OR theat?? OR disorder OR clinical OR stroke OR animal OR dent* OR material OR recogni* OR recommend*))

Appendix B

Exploratory moderator analysis with study quality

Based on the RoB 2, all studies in the meta-analysis were of moderate quality, since the lack of pre-specified analysis plans from included studies made it difficult to completely rule out bias from the selection of reported results. Exploratorily, we conducted a less stringent assessment of study quality assuming all studies contained no bias due to selection of results. Based on this assessment, 7 (50%) of the included studies were high quality, while the remaining were moderate quality.

Following our procedure for moderator analyses, we conducted an additional random-effects meta-regression with RVE to test whether the estimated effect of music listening on stress recovery was stable across studies of different quality. The meta-regression suggests that study quality is a significant moderator of the effect of music listening on stress recovery, QM(1) = 41.95, p < .001. The estimated effect of music listening on stress recovery in high quality studies was g = 0.178, 95% CI [0.00, 0.35], p = .046, while the estimated effect of music in moderate quality studies was g = 0.102, 95% CI [-0.14, 0.35], p = .041.

Appendix C

Stress induction procedures

In our meta-analysis, we generally distinguished between stress induction procedures with- or without a socio-evaluative threat component. However, specific stress induction procedures varied considerably between studies, as described below:

Arithmetic tasks

Four studies utilized arithmetic tasks to induce stress in participants. These tasks included single- and double-digit mental arithmetic operations [17], mental arithmetic operations “with harassment” [18, 21], and standardized mathematic tests [84].

Trier Social Stress Task (with modifications)

One study [54] followed the standard administration protocol of the Trier Social Stress Task (TSST) [109, 116]. Two studies modified the TSST [109] by having participants prepare and deliver their presentations in front of a camera instead of a panel of judges [3, 37], while the subsequent mental arithmetic task was replaced by the Paced Auditory Serial Addition Test (PASAT) [117], administered through a laptop. One study administered the TSST with a shorter mental arithmetic component [118], while two studies omitted the TSST’s speech delivery component [119, 120].

Anticipation

One study made use of anticipation to induce stress [50], where participants were asked to prepare an impromptu presentation that would be videotaped at the end of a preparation period. Participants were eventually not required to deliver the prepared presentation.

Unpleasant stimuli

Two studies exposed participants to unpleasant stimuli as a means of inducing stress. These unpleasant stimuli were either auditory [82] or visual [19] in nature.

CO2 stress task

One study utilized the CO2 Stress Task [61]. In this task, as a an acute physiological stressor, participants were instructed to take a single, vital-capacity breath of air containing 35% carbon dioxide and 65% oxygen [111].

The duration of each stress induction procedure varied according to procedure category. The longest stress induction procedures (15 minutes) typically involved (variations of) the TSST (e.g., [37]. Conversely, the shortest procedure (90 seconds) was the exposure to unpleasant noise in Nakajima et al. [82], as their experimental design involved repeated presentation of the stressor to participants. Finally, it is also worth noting that among studies which reported successful stress induction procedures (see Table 2), the magnitude of resulting stress responses was often not reported.

Selection of musical stimuli

All studies held a general assumption that ‘relaxing’ music would best promote stress recovery. However, studies utilized different strategies in selecting ‘relaxing’ music, resulting in considerable variation in musical stimuli between studies. These strategies are listed below:

Sampling from available music

Four studies utilized a relatively straightforward strategy in selecting music—musical stimuli were sampled from songs commonly found on ‘relaxing’, either from their inclusion in anti-stress cassettes [21, 54], coverage in popular media [120], or the researcher’s opinion [118].

Referencing prior studies

Three studies selected music that, in prior studies, seemed to have positive effects on heart rate, respiration rate, perceived arousal, and perceived relaxation. One study made reference to pilot studies [82], while the remaining two cited previous published work by the same authors [19, 50].

Theoretical conceptualization

Two studies attempted to theoretically conceptualize which music would be ‘relaxing’, and selected their musical stimuli accordingly. De la Torre-Luque et al. [3] utilized Melomics, a computational system for the automatic composition of music, to create songs that would be considered ‘relaxing’. These songs were slow-paced, instrumental pieces, which contained no sudden or abrupt changes in melody. Gan et al. [84] distinguished between stimulative and sedative (‘relaxing’) music based on musical tempo—the speed or pace of a given song, and dynamic range—the difference between the quietest and loudest parts of a song [121]. In their study, stimulative music was characterized by fast tempo and broad dynamic range, while sedative music was characterized by slow tempo and narrow (soft) dynamic range.

Self-selection

Six studies allowed participants to select and listen to their own ‘relaxing’ music. In four studies, participants were instructed to bring a list of ‘preferred’ relaxing music, which they would have the opportunity to listen to during the study [18, 37, 54, 66, 119]. In one study, participants selected ‘relaxing’ music from a list created by the experimenters (pseudo self-selection) [21]. The specific musical stimuli chosen by participants in studies allowing self-selection were often not reported.

Effects of music listening on stress recovery

Studies utilized a variety of outcomes to investigate the effects of music listening on stress recovery. To expand upon the results of our meta-analysis, we detail the findings reported for each of these outcomes below. Given that three studies included in the systematic review could not be included in the meta-analysis due to incomplete reported data, the number of studies per outcome reported in this section may differ from the number of studies per outcome in the meta-analysis (Table 3).

Heart rate

Scheufele [50] reported that participants who listened to classical music demonstrated lower post-stressor heart rate (HR) compared to participants in a comparable control group. By contrast, six studies reported no significant differences in post-stressor HR between participants who listened to music and those who did not [3, 18, 19, 21, 66, 84]. In summary, only one study out of seven provides evidence in support of a positive effect of music listening on post-stressor HR recovery.

Heart rate variability

Four studies utilized various heart rate variability (HRV) indices as a means to assess stress recovery. Two studies reported higher post-stressor HF band power in participants who listened to music compared to those who sat in silence [3, 37]. In Nakajima et al. [82], this difference was more pronounced for participants who listened to music with boosted high frequencies. Contrarily, in Sokhadze [19], participants who listened to peaceful music demonstrated lower post-stressor HF band power compared to those who sat in silence. Two studies reported that post-stressor sample entropy was higher for participants who listened to music compared to silence [3, 37]. This difference was taken as indicator which suggested that the physiological parameters of participants in the music condition were more ready to change compared to those in the silence condition [3]. No studies reported significant differences in RMSSD, LF band power, and LF/HF ratio between participants who listened to music and those who did not [3, 19, 37, 82]. Overall, three of four studies provide support for a positive effect of music listening on post-stressor HRV recovery, but these effects seem to vary across HRV indices.

Blood pressure

Four studies assessed the impact of music listening on stress recovery through changes in systolic blood pressure (SBP) and diastolic blood pressure (DBP). Chafin et al. [21] reported that the post-stressor SBP approached baseline values more quickly for participants who listened to experimenter-selected classical music compared to participants who sat in silence. On the other hand, three studies reported no significant differences in post-stressor SBP between participants who listened to music and those who did not [18, 84, 120]. Instead, compared to participants sitting in silence, post-stressor SBP recovery in participants who listened to either happy or relaxing music was delayed [18]. With regards to DBP, none of the above studies reported significant differences in post-stressor DBP between their respective experimental conditions. In summary, one study out of four provides support for a beneficial effect of music listening on post-stressor SBP recovery, while no studies provide support for a beneficial effect of music listening on DBP recovery.

Respiration rate

One study reported no significant differences in post-stressor respiration rate (RR) between participants listening to different musical genres and silence [17]. As such, there is currently no evidence to suggest that music listening is beneficial for post-stressor RR recovery.

Skin conductance

In Sokhadze [19], participants’ SC was lower while listening to pleasant music compared to during the stressor. In Fallon et al. [118], participants who listened to self-selected music experienced lower SC compared to those in the control condition during the recovery session of the study. In a post-hoc analysis, Labbé et al. [17] reported that post-stressor SC recovery was greater for the classical and self-selected music groups, compared to the heavy metal or no music groups. Collectively, three studies provide evidence for a positive effect of music listening on post-stressor SC recovery.

Cortisol

Two studies utilized cortisol to examine the effect of music listening on stress recovery. Khalfa et al. [54] reported that post-stressor cortisol decreased more rapidly for participants who listened to experimenter-selected classical music, compared to participants who sat in silence. Contrarily, Koelsch et al. [61] reported that music listening delayed cortisol recovery, as cortisol concentrations were higher for participants who listened to music post-stressor compared to silence. As such, only one study out of two provides support for a beneficial effect of music listening on post-stressor cortisol recovery.

Subjective stress

In Groarke & Hogan [119], participants who listened to self-selected music reported lower subjective stress post-stressor compared to those who listened to a radio documentary. By comparison, in Radstaak et al. [18], there were no differences in post-stressor subjective stress between participants listening to happy music, relaxing music, an audiobook, and silence. Thus, only one study out of two provides support for a beneficial effect of music listening on post-stressor subjective stress.

Perceived relaxation

In Labbé et al. [17], post-stressor perceived relaxation was higher for participants who listened to classical music compared to heavy metal, but not compared to silence. There were no significant differences in post-stressor perceived relaxation between participants listening to the various musical genres in Chafin et al. [21], and between participants listening to classical music or silence [50]. Thus, no studies provide conclusive evidence that music listening is beneficial for post-stressor perceived relaxation. However, the effects of music listening on perceived relaxation may differ depending on genre.

State anxiety

Three studies reported that music listening reduced post-stressor state anxiety compared to silence [17, 37, 119]. Furthermore, Gan, Lim, and Haw [84] reported that post-stressor changes in mathematics-related anxiety were significantly higher for participants who listened to sedative music compared to those who did not. Despite this, three studies reported no significant differences in post-stressor state anxiety between their respective experimental groups [3, 19, 21]. Thus, four of seven studies provide support for a beneficial effect of music listening on post-stressor state anxiety.

State depression

Two studies looked at the presence and/or severity of depressive symptoms in order to assess whether or not music facilitated psychological recovery [19, 37]. However, only de la Torre-Luque et al. [37] reported significant positive differences in post-stressor depressive symptoms between participants who listened to music and those who did not.

Rumination

Two studies measured rumination as an indicator of psychological stress recovery, and both reported no significant differences in post-stressor rumination between participants in their respective experimental conditions [18, 21]. As such, there is currently no evidence to suggest that music listening is beneficial for post-stressor rumination.

Positive and negative affect

De la Torre-Luque et al. [37] noted that participants who listened to music reported higher positive affect scores and lower negative affect scores post-stressor compared to the control group. Similarly, Radstaak et al. [18] reported that participants who listened to happy or relaxing music reported higher post-stressor positive affect compared to participants who did not listen to music, but found no significant differences in post-stressor negative affect. Two studies utilized the Profile of Moods Scale (POMS) to assess post-stressor changes in affect. Koelsch et al. [61] noted that participants who listened to music demonstrated higher post-stressor POMS scores (suggesting higher positive affect) compared to those who sat in silence. On the other hand, Scheufele [50] reported no significant differences in post-stressor POMS scores between experimental groups. Two studies [118, 119] measured affect by asking participants to report whether they felt various emotions (e.g., calmness, nervousness) throughout the study. Fallon et al. [118] reported that music listening did not have differential effects on affect compared to silence, while Groarke and Hogan [119] noted that participants who listened to music demonstrated less negative affect (as indicated by lower scores on the various emotions that participants were asked to rate) compared to those who did not. Collectively, the effect of music listening on post-stressor positive and negative affect seemed to be mixed. Three studies provide support for the beneficial role of music listening on post-stressor positive affect, and two studies provide support for the beneficial effect of music listening for negative affect.

Supporting information

S1 Checklist

(DOC)

Data Availability

All materials relevant to our review, including: (a) the pre-registered study protocol; (b) an outline of the search strategy; (c) a list of screened articles with rationales for exclusion; (d) the meta-analysis data set with extracted data; and, (e) R code to replicate the analysis, are available on the Open Science Framework (https://osf.io/9pxhj/?view_only=0f2f28db4adf4a2492aa57e5e003cc9f).

Funding Statement

The author(s) received no specific funding for this work.

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Decision Letter 0

Urs M Nater

22 Oct 2021

PONE-D-21-22806Music listening and stress recovery in healthy individuals: A systematic review with meta-analysis of experimental studiesPLOS ONE

Dear Dr. Adiasto,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

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Reviewer #1: Yes

Reviewer #2: Partly

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: This systematic review and meta-analysis examined the effects of music listening after experimentally induced stress on stress recovery in healthy participants. The authors found no evidence for a cumulative effect of music listening on stress recovery. They did find that the effectiveness of music was moderated by several factors, including musical genre, type of music selection, musical tempo, and type of stress recovery outcome, although definite conclusions on the nature of these effects could not be drawn.

The study addresses a very timely question within the growing body of research on music and stress, and is well-executed. As such, it provides a valuable and much-needed contribution to the research field. The selection of study parameters and moderators of interest is convincing, the results are presented in a clear and comprehensible way, and the authors provide a thoughtful interpretation of their findings, which they appropriately put into perspective by acknowledging the limitations of their study. Furthermore, they provide several helpful and well-considered recommendations for future research. The manuscript is well-written.

I only have several minor comments.

1. In discussing the potential moderating effect “Self- vs. experimenter selected” (page 9), the authors mention two presumed explanations for this effect, namely increasing perceived control and serving self-regularity goals. For a somewhat more comprehensive picture, it may be worth adding the potential roles of liking and familiarity as further mechanisms behind the suggested higher effectiveness of self-compared to experimenter-selected music in promoting stress recovery.

2. In the abstract (and throughout the theoretical sections of the paper), it is stated that participants of the studies included in the meta-analysis/review “were either exposed to music or silence.” I find this misleading, since in the Method section, it is stated on page 10 that to be included, “studies should compare music listening to silence or a comparable auditory stimulus (e.g., white noise, audiobooks)”.

Apart from the fact that it is not evident in what sense and to what extent silence can be considered comparable to auditory control stimuli, the use of the label “silence” to capture all non-music control conditions, is confusing. Please adapt the instances where you currently refer to silence by using more accurate wording (e.g. “silence or an auditory control condition”).

3. Page 11: “When authors did not or could not provide the required information (e.g., due to data no longer being accessible), the outcome was dropped from the meta-analysis. Based on these criteria, the final sample for the systematic review consisted of 17 studies. Following attempts to obtain missing information, the final sample for the meta-analysis consisted of 14 studies.”

This is phrased in a confusing way – it is not clear what the conceptual difference between these selection steps is. Please rephrase this in a way that makes it less confusing.

4. On page 11, the authors point out that “Stress induction procedures in included studies were not always successful. Given that successful stress induction procedures are crucial to ensure that participants experience some physiological or psychological change they may recover from, in our moderator analysis we examined whether the effect of music listening on stress recovery differed based on the outcome of a study’s stress induction check (manipulation check)”.

I fully agree with the authors that, for music to exert an effect on stress, a physiological and/or psychological stress response needs to be present, from which participants may then recover. I find it therefore difficult to understand why studies which failed to induce stress (i.e. did not report a successful stress induction) were included in the meta-analysis in the first place. The fact that the successfulness of the stress induction, surprisingly, did not affect the extent of stress recovery does not really resolve my concern.

Could the authors briefly comment on this issue, and motivate their decision to still include these studies in their meta-analysis (either under “stress induction checks” on page 11, or in the discussion section)?

5. Page 11: “In our moderator analysis, we examined whether the effects of music listening on stress recovery were reliable across general (neuroendocrine, physiological, psychological) and specific outcome types.”

I am not sure whether the moderator analysis allows any claims about the reliability of the effects across outcome types. In theory, an effect could be highly reliable across many outcome types, while at the same time still being clearly stronger for some outcome types than for others (hence being moderated by them), right? Wouldn’t it be more correct to state that it was assessed to what extent the size of the effect on stress recovery depended on outcome type (or some equivalent formulation)? I am no expert on this issue, but I invite the authors to reconsider their wording.

6. There is a type on page 15: Wisagreements --> Disagreements

7. As the authors rightly point out on page 6, stress recovery involves a process in which “changes that have occurred in response to a stressor revert to pre-stress baselines”. To quantify stress recovery, it therefore seems crucial to take individual pre-stress baseline levels into account.

To the reader, it does not readily become clear whether the effects derived from the studies included in the meta-analysis indeed reflect the extent to which stress levels “return to baseline”. From Table 4, the included studies seem to be a mix of 1) studies reporting differences in change scores with respect to pre-stress baseline levels and 2) studies reporting raw group differences in post-music stress levels. This may require some sort of disclaimer.

Could you please reflect on these analytical differences and their (possible) implications for the interpretation of your meta-analysis, in relation to the term “recovery”?

8. On page 37-38 you write: “Khalfa et al. [55] reported that post-stressor cortisol decreased more rapidly for participants who listened to experimenter-selected classical music, compared with participants who sat in silence”

In Table 2 you write, when referring to this study: “Increase in post-stressor cortisol for music group significantly lower compared to control group (+)”.

These descriptions differ – could you please adapt the main text to match the (correct) description in the table?

9. On page 40-41, you write: “While previous reviews suggest that music-based interventions may be moderately beneficial for stress-related outcomes, particularly in medical and therapeutic settings, our results suggest that the magnitude of this effect for healthy individuals may be more modest.”

While I largely agree with the contents of this paragraph (and with the further comments on this issue on page 46), I think the term “healthy individuals” (to label the category for which music is less effective for stress recovery) does not capture the essence of the differences between the different types of studies, and hence using this term may be a bit misleading.

As is stated further down the paragraph, the stress in studies conducted in medical and therapeutic settings likely has a more protracted time course, which does not directly have to do with the participants being (not) healthy. Furthermore, stress may differ in intensity between laboratory and medical real-life/settings, and the effectiveness of music may depend on the research setting as well.

It would be great if you could somewhat adapt the wording of this paragraph, to avoid the impression that the (non-) effectiveness of music depends on the participants being healthy. Rather, it seems more likely that several (interrelated) factors associated with the different research settings (e.g. type, intensity and duration of stress) are driving these differences. You might e.g. use the term “healthy individuals under brief, experimentally induced stress” instead.

Reviewer #2: Review:

The authors address an important research question as they aim at systemizing the empirical evidence on beneficial effects of music listening on stress recovery. Overall, the manuscript is well written and the authors demonstrate methodological rigour and diligence on many instances. However, I have some major concerns that question the adequacy of the hypothesis and statistical approach as well as the search strategy.

Major Concerns:

1) The authors identified 14 studies that are quite heterogenous in nature. I ask myself whether the approach of a meta-analysis is adequate for this rather small number of studies given this vast heterogenity. Furthermore, not all studies were successfull in stress induction. Wouldn't it be reasonable to exclude these studies from the analysis?

2) Concerning the search strategy, I wondered that PubMed was not included. Searching this data base might be useful as the number of studies identified is quite small.

3) Inclusion and exclusion criteria are not specifically justified. For example, in Figure 1 exclusion criteria are presented, e.g. 'no music presented after stressor'. This might be the reason why the study of Thoma et al. (2013) (https://pubmed.ncbi.nlm.nih.gov/23940541/) is not party of the review, although I consider it highly relevant in the context of music listening and stress reduction. Overall, I would describe and justify more in detail criteria for inclusion and exclusion of studies.

4) Introduction: first paragraph: I really like the introduction to the topic, as the aspect of stress in daily life is emphasized. I wondered why the authors did not include ambulatory assessment/ecological momentary assessment studies in their review, as these studies have high ecological validity. I recommend to expand the review and to assess independently the evidence concerning controlled studies with high internal validity on the one hand and daily life studies with high ecological validity on the other hand.

5) It may be my personal opinion, but I was irritated by the many instances the authors use the term 'beliefs', e.g. abstract 'given the popular and widespread belief'. My recommendation is to re-word this and acknowledge the empirical evidence underlying this statement.

Minor Concerns:

Abstract: 'beneficial for stress' should be specified (beneficial for stress reduction)

Abstract: Please report how many participants in total were included in the 14 studies

Abstract: please specify that (randomized)-controlled studies were included

Introduction: 'It is a popular and widespread opinion that music may be beneficial for

stress recovery [10]': I am not convinced that the citation is that adequate in this context. Levitin demonstrates in this book many instances for beneficial effects of music. I am not satisfied with labeling his statement as 'popular and widespread opinion'. Furthermore, I do not consider this book an optimal citation for this peer-reviewed journal article as there is a vast body of empirical evidence available.

Introduction l.60: My first thought was to question the necessity of this review given the fact that an extensive review was just published. Although, the authors justify their review in the ensuing paragraph, I would recommend to state immediately more clearly that the scope of the other review was different.

Introduction l.78: Why not include studies demonstrating effects on cortisol?

l.133: I would not recommend to write 'smaller amounts of salivary alpha-amylase'. Rather, less activity of alpha-amylase.

l.153: Study 54 refers to an ambulatory assessment study – therefore, there was no control to silence or noise in a comparable manner to experimental studies. Please re-word.

l.164: I do not agree with the statement that the candidate mechanism underlying beneficial effects of music has already been identified. I would rather prefer to see here a more comprehensive statement acknowledging that the exact underlying mechanisms remain to be elucidated and that different notions exist, e.g., literature by Koelsch…

l.274: IgA is named as outcome measure but has not been introduced. As it is an immune marker, the introduction should contain some information on interactions among stress and immune system.

l.310: some typos need revision

l.425: Can you please indicate the range of music duration? As there is literature available on the effects of different music durations on beneficial effects, I assume that the range was very limited among these studies. Therefore, I would not state that duration is not important. Rather, that the range in experimental studies is not vast enough to allow for meaningful comparisons.

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Reviewer #1: No

Reviewer #2: No

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PLoS One. 2022 Jun 17;17(6):e0270031. doi: 10.1371/journal.pone.0270031.r002

Author response to Decision Letter 0


17 Dec 2021

Dear Prof. Dr. Nater,

Thank you for your e-mail in response to our submission to PLOS ONE [PONE-D-21-22806], in which you explained your decision concerning our manuscript, enclosing the reviewers’ comments. We have carefully addressed each reviewer’s comments and adjusted our manuscript accordingly. Our responses to each reviewer’s comments have been provided in a point-by-point manner below.

We hope the revised version of our manuscript will fully meet PLOS ONE’s rigorous publication criteria. We look forward to hearing from you.

Warm regards,

Krisna Adiasto, MSc.

On behalf of all authors.

---------------

Response to Reviewer #1

Dear Reviewer,

Thank you for taking the time to review our manuscript. We appreciate your kind words about our work. More importantly, we sincerely appreciate the constructive comments and suggestions you have provided for various portions of our review and have thus updated our manuscript to address them. Below, we provide a summary of the changes we have made. In the response letter we have attached to our revision, you will see your comments in bold, our responses in regular text, and excerpts from our manuscript in italics (unless otherwise indicated).

1. In discussing the potential moderating effect “Self- vs. experimenter selected” (page 9), the authors mention two presumed explanations for this effect, namely increasing perceived control and serving self-regularity goals. For a somewhat more comprehensive picture, it may be worth adding the potential roles of liking and familiarity as further mechanisms behind the suggested higher effectiveness of self-compared to experimenter-selected music in promoting stress recovery.

We agree that preference and familiarity potentially play a role in making self-selected music more beneficial compared to experimenter selected music for the purpose of stress recovery. As we strive to make our review as comprehensive as possible, we have added the following sentences on the roles of preference and familiarity in our discussion of the potential moderating effects of self- vs. experimenter selected music (page 9):

“Furthermore, previous studies have found that listening to self-selected music may help elicit stronger and more positive emotional responses regardless of a song’s valence (positive or negative) and arousal (high or low), possibly due to increased preference and familiarity towards the self-selected music (Jiang et al., 2016; Pereira et al., 2011; Sharman & Dingle, 2015). In theory, self-selected music should thus be more beneficial compared to experimenter-selected music for the purpose of stress recovery.”

And again in our discussion of the moderator analyses (page 45):

“The comparison of musical features between self-selected and experimenter selected music may also offer a more nuanced perspective on the role of preference and familiarity. Specifically, preferences and familiarity towards certain songs could be described in terms of specific (combinations of) musical features. For example, an individual may prefer music with slow tempo, mellow timbre, and moderate loudness. This approach is often leveraged by music recommender systems, such as those implemented by music streaming platforms (e.g., Spotify, Deezer, Apple Music, etc.), with the goal of recommending songs that listeners are likely to engage with. Future studies could investigate the extent to which preference and familiarity might differ between self-selected and experimenter selected music with similar combinations of musical features, to further clarify the role of selection in the relationship between music listening and stress recovery.”

Jiang, J., Rickson, D., & Jiang, C. (2016). The mechanism of music for reducing psychological stress: Music preference as a mediator. The Arts in Psychotherapy, 48, 62-68. https://doi.org/10.1016/j.aip.2016.02.002

Pereira, C. S., Teixeira, J., Figueiredo, P., Xavier, J., Castro, S. L., & Brattico, E. (2011). Music and emotions in the brain: Familiarity matters. PLOS ONE, 6(11), e27241. https://doi.org/10.1371/journal.pone.0027241

Sharman, L., & Dingle, G. A. (2015). Extreme metal music and anger processing. Frontiers in Human Neuroscience, 9, 272. https://doi.org/10.3389/fnhum.2015.00272

2. In the abstract (and throughout the theoretical sections of the paper), it is stated that participants of the studies included in the meta-analysis/review “were either exposed to music or silence.” I find this misleading, since in the Method section, it is stated on page 10 that to be included, “studies should compare music listening to silence or a comparable auditory stimulus (e.g., white noise, audiobooks)”.

Apart from the fact that it is not evident in what sense and to what extent silence can be considered comparable to auditory control stimuli, the use of the label “silence” to capture all non-music control conditions, is confusing. Please adapt the instances where you currently refer to silence by using more accurate wording (e.g., “silence or an auditory control condition”).

Thank you for your suggestion. It was not our intention to use the label ‘silence’ as an overall term for all control conditions across studies. When the change was justified (i.e., when the cited studies indeed had an auditory control condition), we have adjusted the instances in which use of the label ‘silence’ only was not accurate. For example, in the Abstract:

“As such, to clarify the current literature, we conducted a systematic review with meta-analysis of randomized, controlled experimental studies investigating the effects of music listening on stress recovery in healthy individuals. In fourteen experimental studies, participants (N = 706) were first exposed to an acute laboratory stressor, following which they were either exposed to music or a control condition.”

On page 4:

“Indeed, studies on music listening and stress recovery in healthy individuals are equivocal: although music listening is considered beneficial for physiological stress recovery, several studies have reported no differences in heart rate, heart rate variability, respiration rate, or blood pressure recovery between participants who listened to music and those who either sat in silence or listened to an auditory control [18-21].”

On page 7:

“For example, music listening has been associated with lower heart rate [49-51], systolic blood pressure [22, 50, 52], skin conductance [18, 20, 53, 54], and cortisol [55] compared to silence or an auditory control condition.”

“Furthermore, studies have demonstrated that listening to music may influence mood [60, 61]. Indeed, music listening has been associated with lower negative affect [38], higher positive affect [19, 62], and fewer self-reported depressive symptoms [38] compared to silence or an auditory control condition.”

On page 16:

“In the present study, a Hedges’ g of zero indicates the effect of music listening on stress recovery is equivalent to silence or an auditory control. Conversely, a Hedges’ g greater than zero indicates the degree to which music listening is more effective than control, while a g less than zero indicates the degree to which music listening is less effective than control.”

On page 22:

“This estimate suggests that, taking all variations in music and outcomes into consideration, the effect of music listening and silence have equivalent effects on stress recovery is equivalent to silence or an auditory control.”

3. Page 11: “When authors did not or could not provide the required information (e.g., due to data no longer being accessible), the outcome was dropped from the meta-analysis. Based on these criteria, the final sample for the systematic review consisted of 17 studies. Following attempts to obtain missing information, the final sample for the meta-analysis consisted of 14 studies.”

This is phrased in a confusing way – it is not clear what the conceptual difference between these selection steps is. Please rephrase this in a way that makes it less confusing.

Thank you for pointing this out. To make the distinction between the two steps clearer, we have rephrased the sentences in question to:

“Based on these criteria, the final sample for the systematic review portion of our manuscript consisted of 17 studies. Finally, for studies to be included in the meta-analysis portion of our review, means and standard deviations of stress recovery outcomes following stressor cessation must be available. Corresponding authors were contacted when this information was not available. When authors did not or could not provide the required information (e.g., due to data no longer being accessible), the outcome was dropped from the meta-analysis. Thus, following attempts to obtain missing information, the final sample for the meta-analysis portion of our review consisted of 14 studies.”

4. On page 11, the authors point out that “Stress induction procedures in included studies were not always successful. Given that successful stress induction procedures are crucial to ensure that participants experience some physiological or psychological change they may recover from, in our moderator analysis we examined whether the effect of music listening on stress recovery differed based on the outcome of a study’s stress induction check (manipulation check)”.

I fully agree with the authors that, for music to exert an effect on stress, a physiological and/or psychological stress response needs to be present, from which participants may then recover. I find it therefore difficult to understand why studies which failed to induce stress (i.e., did not report a successful stress induction) were included in the meta-analysis in the first place. The fact that the successfulness of the stress induction, surprisingly, did not affect the extent of stress recovery does not really resolve my concern.

Could the authors briefly comment on this issue and motivate their decision to still include these studies in their meta-analysis (either under “stress induction checks” on page 11, or in the discussion section)?

Thank you for mentioning this. We agree that it is particularly important to further address the inclusion of studies with unsuccessful stress induction checks, as you and another reviewer have put forward similar concerns on the matter.

In the previous version of our manuscript, three studies were coded to have ‘unsuccessful’ stress induction checks. One of these studies (Scheufele, 2000) was erroneously coded, as the author did report a marked increase in heart rate following their stress induction procedure compared to baseline. Meanwhile, in the remaining two studies (Gan et al., 2016 & Labbé et al., 2007), the authors have hinted that their respective stress induction procedures were successful. However, we coded these as ‘unsuccessful’ since the statistical analyses comparing post-stressor and baseline values of their stress recovery outcomes were missing. We ultimately decided to still include these two studies in the meta-analysis given that the reported mean scores for certain stress recovery outcomes still suggested there was an increase from baseline from which participants could recover from. For example, in Gan et al. (2016), mean state anxiety for their three conditions during the stress task were msedative = 46.97, mstimulative = 50.51, and mcontrol = 52.00, compared to baseline means scores of msedative = 38.80, mstimulative = 38.09, and mcontrol = 36.17.

Based on this, we have thus updated the results of our analysis for the “stress induction checks” moderator (page 27), to reflect the change in coding for Scheufele (2000):

“Stress induction checks. There were no significant differences in the effects of music listening on stress recovery for studies with successful (g = 0.17301, 95% CI [-0.2635, 0.6155], p = .399625) and unsuccessful (g = 0.062361, 95% CI [-0.0894, 0.201.66], p = .115355) stress induction checks, β1 = -0.108257, p = .525661.”

Next, we have added a paragraph to acknowledge the inclusion of studies with unsuccessful stress tasks in our Discussion (page 49):

“Two studies with less successful stress induction procedures were still included in the review, given that reported raw scores for certain recovery outcomes still suggested an increase from baseline that participants could recover from. For example, in Gan et al. (2016), mean state anxiety for their three conditions during the stress task were msedative = 46.97, mstimulative = 50.51, and mcontrol = 52.00, compared to baseline means scores of msedative = 38.80, mstimulative = 38.09, and mcontrol = 36.17. Given that the overall estimated effect of music listening on the recovery process of healthy individuals following laboratory stressors may be relatively modest, it becomes particularly important to ensure that a sufficient stress response is elicited, to provide a larger window of opportunity in which the effect of music listening may be exerted on participants’ recovery processes. We thus encourage future studies to adopt validated (variations of) well-known stress tasks, such as the TSST, SECPT, or CO2 stress task, which have been demonstrated to consistently elicit marked physiological and psychological stress-related responses in laboratory settings.”

Gan, S. K. E., Lim, K. M. J., & Haw, Y. X. (2016). The relaxation effects of stimulative and sedative music on mathematics anxiety: A perception to physiology model. Psychology of Music, 44(4), 730-741. https://doi.org/10.1177/0305735615590430

Labbé, E., Schmidt, N., Babin, J., & Pharr, M. (2007). Coping with stress: the effectiveness of different types of music. Applied Psychophysiology and Biofeedback, 32, 163-168. https://doi.org/10.1007/s10484-007-9043-9

Scheufele, P. M. (2000). Effects of progressive relaxation and classical music on measurements of attention, relaxation, and stress responses. Journal of Behavioral Medicine, 23, 207-228. https://doi.org/10.21236/ad1012237

5. Page 11: “In our moderator analysis, we examined whether the effects of music listening on stress recovery were reliable across general (neuroendocrine, physiological, psychological) and specific outcome types.”

I am not sure whether the moderator analysis allows any claims about the reliability of the effects across outcome types. In theory, an effect could be highly reliable across many outcome types, while at the same time still being clearly stronger for some outcome types than for others (hence being moderated by them), right? Wouldn’t it be more correct to state that it was assessed to what extent the size of the effect on stress recovery depended on outcome type (or some equivalent formulation)? I am no expert on this issue, but I invite the authors to reconsider their wording.

Thank you for your comment. Indeed, we utilized the term ‘reliable’ when in fact our intention was to assess the extent to which the size of the effect of music listening on stress recovery would differ, for example across outcome types. We have thus adjusted our wording to the following (page 12):

“In our moderator analysis, we examined whether the effects of music listening on stress recovery differed across general (neuroendocrine, physiological, psychological) and specific outcome types.”

6. There is a type on page 15: Wisagreements � Disagreements

Thank you for pointing this out. The typo (page 15) has been fixed.

“Disagreements were resolved through face-to-face discussions, or through consultation with SG and KR when no consensus could be reached.”

7. As the authors rightly point out on page 6, stress recovery involves a process in which “changes that have occurred in response to a stressor revert to pre-stress baselines”. To quantify stress recovery, it therefore seems crucial to take individual pre-stress baseline levels into account.

To the reader, it does not readily become clear whether the effects derived from the studies included in the meta-analysis indeed reflect the extent to which stress levels “return to baseline”. From Table 4, the included studies seem to be a mix of 1) studies reporting differences in change scores with respect to pre-stress baseline levels and 2) studies reporting raw group differences in post-music stress levels. This may require some sort of disclaimer.

Could you please reflect on these analytical differences and their (possible) implications for the interpretation of your meta-analysis, in relation to the term “recovery”?

Previous research has shown that following an acute stress reaction, all elevated physiological and psychological parameters will naturally revert to pre-stress baselines within 30-60 minutes (Hermans et al., 2014). As such, the most immediate proof of the effect of music listening on stress recovery would be to see whether listening to music would allow participants to reach their respective baseline levels sooner within time frame. Unfortunately, as we also point out in our Discussion, it is rare for studies to adopt a design where such changes are monitored, particularly through use of continuous measures. Instead, as you have rightly pointed out, studies either compare post-stress and post-manipulation change scores between conditions or compare post-manipulation raw group differences between music and comparable control conditions. The effects of music listening on stress recovery that we describe in our meta-analysis thus reflect how reactive participants’ stress recovery processes are when listening to music, rather than how soon participants recover, with the assumption that greater reactivity (e.g., larger decreases in heart rate) post-stressor also results in earlier returns to baseline.

Hermans, E. J., Henckens, M. J., Joëls, M., & Fernández, G. (2014). Dynamic adaptation of large-scale brain networks in response to acute stressors. Trends in Neurosciences, 37, 304-314. https://doi.org/10.1016/j.tins.2014.03.006

8. On page 37-38 you write: “Khalfa et al. [55] reported that post-stressor cortisol decreased more rapidly for participants who listened to experimenter-selected classical music, compared with participants who sat in silence”

In Table 2 you write, when referring to this study: “Increase in post-stressor cortisol for music group significantly lower compared to control group (+)”.

These descriptions differ – could you please adapt the main text to match the (correct) description in the table?

Thank you for pointing this out. The correct description was what we wrote in text. We have thus adjusted the description in Table 4 to:

“Significant, rapid decrease in post-stressor cortisol in music group compared to control group (+).”

9. On page 40-41, you write: “While previous reviews suggest that music-based interventions may be moderately beneficial for stress-related outcomes, particularly in medical and therapeutic settings, our results suggest that the magnitude of this effect for healthy individuals may be more modest.”

While I largely agree with the contents of this paragraph (and with the further comments on this issue on page 46), I think the term “healthy individuals” (to label the category for which music is less effective for stress recovery) does not capture the essence of the differences between the different types of studies, and hence using this term may be a bit misleading.

As is stated further down the paragraph, the stress in studies conducted in medical and therapeutic settings likely has a more protracted time course, which does not directly have to do with the participants being (not) healthy. Furthermore, stress may differ in intensity between laboratory and medical real-life/settings, and the effectiveness of music may depend on the research setting as well.

It would be great if you could somewhat adapt the wording of this paragraph, to avoid the impression that the (non-) effectiveness of music depends on the participants being healthy. Rather, it seems more likely that several (interrelated) factors associated with the different research settings (e.g., type, intensity, and duration of stress) are driving these differences. You might e.g., use the term “healthy individuals under brief, experimentally induced stress” instead.

Thank you for your comment. Indeed, the term “healthy individuals” may at times oversimplify the fact that the effect of music listening may differ based on differences in research settings. We have reworded the paragraph as follows:

“The results of our review contrast those of previous meta-analyses, which underscore the relevance of music-based interventions for stress-reduction [11, 12]. While previous reviews suggest that music-based interventions may be moderately beneficial for stress-related outcomes, particularly in medical and therapeutic settings, our results suggest that the magnitude of this effect outside of these settings, particularly for healthy individuals under acute, experimentally induced stress, may be more modest. We presume that one of the principal reasons for this difference was our decision to exclude studies conducted in medical and therapeutic settings. In previous reviews, randomized controlled trials of the effects of music-based interventions within medical and therapeutic settings constituted a large portion of included studies: 67 of 79 (85%) studies in de Witte et al. [11], and 15 of 22 (68%) studies in Pelletier [12], making it more likely that overall effect sizes were derived from studies conducted within these settings. Tentatively, the effects of music listening may be more prominent for the stress recovery of individuals in medical or therapeutic contexts, compared to that of individuals under acute stress in an experimental context. Whereas the time course of stress responses and stress recovery in experimental settings can be considered relatively brief [25, 27, 41, 85], the time course of stress responses and stress recovery within medical and therapeutic settings may be significantly more protracted [13, 14]. Thus, within medical and therapeutic settings, music may be exerting its influence on neuroendocrine, physiological, and psychological processes that have been subjected to longer periods of strain [28, 108].”

---------------

Response to Reviewer #2

Dear Reviewer,

Thank you for taking the time to review our manuscript. We appreciate the kind words you have mentioned on the execution of our review. More importantly, we sincerely appreciate the critical comments you have provided on our search strategy and adequacy of our approach. Below, we address your concerns in a point-by-point fashion and summarize the changes we have made to our manuscript based on your suggestions. In the response letter we have included with our revision, you will see your comments in bold, our responses in regular text, and excerpts from our manuscript in italics (unless otherwise indicated):

Major Concerns:

1) The authors identified 14 studies that are quite heterogenous in nature. I ask myself whether the approach of a meta-analysis is adequate for this rather small number of studies given this vast heterogeneity. Furthermore, not all studies were successful in stress induction. Wouldn't it be reasonable to exclude these studies from the analysis?

We acknowledge that the relatively small number of heterogeneous studies may render the results of our meta-analysis less meaningful. We can thus understand if concerns are raised about whether a meta-analysis is the most appropriate approach to synthesize the available empirical evidence on the relationship between music listening and stress recovery.

As we state in the Limitations of our manuscript (page 49), we are aware that the small number of included studies makes it difficult to draw meaningful, substantial conclusions based on the results of the meta-analysis alone. For this reason, we have supplemented the quantitative synthesis of the meta-analysis with a more qualitative synthesis from a systematic review. We think this combined approach has yielded a more nuanced review, as the qualitative description of the included studies have helped provide more context to the results of our meta-analysis. We have reported the systematic review in our Results section on page 27.

Thus, we think our review is still valuable despite the small number of studies, as it provides not only a quantitative synthesis of available evidence, but also provides a qualitative description of the potential sources of heterogeneity that the meta-analysis could not account for.

Next, thank you for mentioning your concern over the inclusion of studies whose stress induction procedures were not successful. A similar point was raised by another reviewer. We thus agree that it is particularly important to further address the inclusion of studies with unsuccessful stress induction checks.

In the previous version of our manuscript, three studies were coded to have ‘unsuccessful’ stress induction checks. One of these studies (Scheufele, 2000) was erroneously coded, as the author did report a marked increase in heart rate following their stress induction procedure compared to baseline. Meanwhile, in the remaining two studies (Gan et al., 2016 & Labbé et al., 2007), the authors have hinted that their respective stress induction procedures were successful. However, we coded these as ‘unsuccessful’ since the statistical analyses comparing post-stressor and baseline values of their stress recovery outcomes were missing. We ultimately decided to still include these two studies in the meta-analysis given that the reported mean scores for certain stress recovery outcomes still suggested there was an increase from baseline from which participants could recover from. For example, in Gan et al. (2016), mean state anxiety for their three conditions during the stress task were msedative = 46.97, mstimulative = 50.51, and mcontrol = 52.00, compared to baseline means scores of msedative = 38.80, mstimulative = 38.09, and mcontrol = 36.17.

Based on this, we have thus updated the results of our analysis for the “stress induction checks” moderator (page 26), to reflect the change in coding for Scheufele (2000):

“Stress induction checks. There were no significant differences in the effects of music listening on stress recovery for studies with successful (g = 0.17301, 95% CI [-0.2635, 0.6155], p = .399625) and unsuccessful (g = 0.062361, 95% CI [-0.0894, 0.201.66], p = .115355) stress induction checks, β1 = -0.108257, p = .525661.”

Next, we have added a paragraph to acknowledge the inclusion of studies with unsuccessful stress tasks in our Discussion (page 48):

“Two studies with less successful stress induction procedures were still included in the review, given that reported raw scores for certain recovery outcomes still suggested an increase from baseline that participants could recover from. For example, in Gan et al. (2016), mean state anxiety for their three conditions during the stress task were msedative = 46.97, mstimulative = 50.51, and mcontrol = 52.00, compared to baseline means scores of msedative = 38.80, mstimulative = 38.09, and mcontrol = 36.17. Given that the overall estimated effect of music listening on the recovery process of healthy individuals following laboratory stressors may be relatively modest, it becomes particularly important to ensure that a sufficient stress response is elicited, to provide a larger window of opportunity in which the effect of music listening may be exerted on participants’ recovery processes. We thus encourage future studies to adopt validated (variations of) well-known stress tasks, such as the TSST, SECPT, or CO2 stress task, which have been demonstrated to consistently elicit marked physiological and psychological stress-related responses in laboratory settings.”

Gan, S. K. E., Lim, K. M. J., & Haw, Y. X. (2016). The relaxation effects of stimulative and sedative music on mathematics anxiety: A perception to physiology model. Psychology of Music, 44(4), 730-741. https://doi.org/10.1177/0305735615590430

Labbé, E., Schmidt, N., Babin, J., & Pharr, M. (2007). Coping with stress: the effectiveness of different types of music. Applied Psychophysiology and Biofeedback, 32, 163-168. https://doi.org/10.1007/s10484-007-9043-9

Scheufele, P. M. (2000). Effects of progressive relaxation and classical music on measurements of attention, relaxation, and stress responses. Journal of Behavioral Medicine, 23, 207-228. https://doi.org/10.21236/ad1012237

2) Concerning the search strategy, I wondered that PubMed was not included. Searching this data base might be useful as the number of studies identified is quite small.

One of the goals of our review was to highlight the overall effect of music listening on stress recovery in healthy individuals. This meant excluding, for example, studies on the effects of music listening in the management of treatment anxiety or stress during pregnancy and labor. As we mention in the Introduction of our review (page 3), we reasoned that the nature of stressors in medical and therapeutic settings, along with their subsequent recovery processes, would be difficult to generalize to more daily settings.

From our experience, most studies on music listening published on the PubMed database reported experiments conducted within medical or therapeutic settings. Thus, when designing our search strategy, we made the decision to exclude the PubMed database from our search.

Despite this, based on your comment, we conducted an additional search in the PubMed database using the same search strategy listed in Appendix A. We limited the additional search to studies published until April 2021 to match our original search. This additional search returned 958 studies, but none of these studies met our inclusion criteria. Our search in PubMed thus resulted in no additional studies.

We have reported this additional search in our manuscript on page 10:

“The results of this primary search were supplemented with three additional electronic searches in the publication databases of Web of Science, PsycINFO, and PubMed. Appendix A provides a complete description of our search terms. Together, this first step resulted in 3124 articles.”

We have also updated Figure 1 to include the addition of the PubMed search:

Information in the paragraph following Figure 1 has also been updated to reflect the additional search:

“During this initial screening, 3008 articles were excluded. KA then scanned the reference lists of the 116 remaining articles for potentially relevant studies, resulting in an additional three articles. Together, this second step resulted in 119 full-text reports to be assessed for eligibility.”

3) Inclusion and exclusion criteria are not specifically justified. For example, in Figure 1 exclusion criteria are presented, e.g. 'no music presented after stressor'. This might be the reason why the study of Thoma et al. (2013) (https://pubmed.ncbi.nlm.nih.gov/23940541/) is not party of the review, although I consider it highly relevant in the context of music listening and stress reduction. Overall, I would describe and justify more in detail criteria for inclusion and exclusion of studies.

Thank you for pointing this out. We agree that our inclusion and exclusion criteria could be better justified. As such, we have described our inclusion and exclusion criteria (pp. 10-11) more extensively, as follows:

“Lastly, KA used the following criteria to assess full-text reports for eligibility:

First, to minimize between-study heterogeneity, and to ensure that included studies investigated the effects of music listening on stress recovery as precisely as possible, studies must employ an experimental design including stress induction, with random assignment of participants to experimental and control conditions. Quasi-experimental studies were included only when they incorporated a control or comparison group. Second, to ensure that included studies tested the immediate effect music listening may have on the stress recovery process, studies should compare music listening to silence or an auditory stimulus (e.g., white noise, audiobooks) following stress induction. Third, to demonstrate this effect, studies must include at least one measure of neuroendocrine (e.g., cortisol), physiological (e.g., heart rate, blood pressure), or psychological (e.g., subjective stress, positive and negative affect) stress recovery outcome. Fourth, given that stress reactivity and recovery responses differ between children and adults, and with consideration to the potential role of music in the prevention of stress-related diseases in adults, studies must include healthy, adult, human participants. Fifth, to improve the generalization of our results in the context of daily stress recovery, studies where stress recovery occurred within a medical or therapeutic context, such as a hospital or operating room, were excluded.”

We agree that the findings of Thoma et al. (2013) are interesting and particularly relevant in the context of music listening and stress reduction. The experiment by Thoma and colleagues convincingly demonstrated that listening to music prior to a stressor resulted in a milder stress response compared to silence, which in turn resulted in a lower need for subsequent recovery. Although their finding speaks to the benefits of music listening in attenuating the stress response, their finding did not completely fit the scope of our review, which was the immediate effect of music listening on recovery from stress.

4) Introduction: first paragraph: I really like the introduction to the topic, as the aspect of stress in daily life is emphasized. I wondered why the authors did not include ambulatory assessment/ecological momentary assessment studies in their review, as these studies have high ecological validity. I recommend to expand the review and to assess independently the evidence concerning controlled studies with high internal validity on the one hand and daily life studies with high ecological validity on the other hand.

When we planned our review, we reasoned that focusing on experimental studies with high internal validity would allow us to examine the strongest available evidence on the presumed relationship between music listening and stress recovery. Furthermore, we hoped that, by focusing on experimental studies, between-study heterogeneity would thus be somewhat minimal – this was eventually not the case.

We agree that there is much to be gleaned from specifically investigating EMA studies on music listening and stress recovery, including further insight into interindividual differences when listening to music for the purpose of stress recovery, and how stress recovery outcomes may be influenced by music listening over time. Despite this, the inclusion of EMA studies in our review would have made it more difficult to determine the immediate effect of music listening on recovery from stress. Given the relatively lower control in EMA studies (e.g., the absence of a clear control condition), claims about causality may be trickier to draw from EMA studies compared to experiments. Furthermore, given that measurements occur outside of the laboratory, it becomes difficult to rule out the effects of contextual variables, particularly when they are not explicitly accounted for in the design of an EMA study. As such, we respectfully argue against the inclusion of EMA studies in our current review, given the stronger ‘causal’ evidence that may be derived from experimental studies, and because we agree that evidence from experimental and EMA studies should be assessed independently of each other due to differences in contextual factors.

5) It may be my personal opinion, but I was irritated by the many instances the authors use the term 'beliefs', e.g., abstract 'given the popular and widespread belief'. My recommendation is to re-word this and acknowledge the empirical evidence underlying this statement.

We apologize for the discomfort we have caused you as you reviewed our manuscript. We agree that, in principle, it is good to acknowledge available empirical evidence rather than labeling a statement a ‘belief’. We have thus adjusted the following instances of the term ‘belief’, starting with the Abstract:

“Studies suggest that listening to music is beneficial for stress reduction. Thus, music listening stands to be a promising method to promote effective recovery from exposure to daily stressors.”

On page 7:

“Furthermore, studies have demonstrated that listening to music may influence mood [59, 60].”

Finally, in our Conclusion:

“Studies commonly suggest that listening to music may have a positive influence on stress recovery”

Minor Concerns:

Abstract: 'beneficial for stress' should be specified (beneficial for stress reduction)

Abstract: please report how many participants in total were included in the 14 studies

Abstract: please specify that (randomized)-controlled studies were included

We have added the above suggestions to the Abstract:

“Studies have suggested that listening to music may be beneficial for stress reduction. Thus, music listening stands to be a promising method to promote effective recovery from exposure to daily stressors.”

“As such, to clarify the current literature, we conducted a systematic review with meta-analysis of randomized, controlled experimental studies investigating the effects of music listening on stress recovery in healthy individuals.”

“In fourteen experimental studies, participants (N = 706) were first exposed to an acute laboratory stressor, following which they were either exposed to music or a control condition.”

Introduction: 'It is a popular and widespread opinion that music may be beneficial for

stress recovery [10]': I am not convinced that the citation is that adequate in this context. Levitin demonstrates in this book many instances for beneficial effects of music. I am not satisfied with labeling his statement as 'popular and widespread opinion'. Furthermore, I do not consider this book an optimal citation for this peer-reviewed journal article as there is a vast body of empirical evidence available.

Thank you for mentioning this. We have rewritten the sentence and provided an alternative reference for it. Below is an excerpt from the paragraph (page 3), with the new sentence highlighted in bold:

“Various activities have been proposed that may lead to better stress recovery, one among them being music listening. Music listening may have a modulatory effect on the human stress response (Thoma et al., 2013). Furthermore, given that music is readily available through online streaming services, music listening stands to be a time- and cost-effective method to facilitate daily stress recovery.”

Thoma, M. V., La Marca, R., Brönnimann, R., Finkel, L., Ehlert, U., & Nater, U. M. (2013). The effect of music on the human stress response. PLOS ONE, 8, e70156. https://doi.org/10.1371/journal.pone.0070156

Introduction l.60: My first thought was to question the necessity of this review given the fact that an extensive review was just published. Although, the authors justify their review in the ensuing paragraph, I would recommend to state immediately more clearly that the scope of the other review was different.

Thank you for your suggestion. We agree that the urgency of our review could be stated earlier in the manuscript. We have restructured the two paragraphs as follows:

“Furthermore, given that music is readily available through online streaming services, music listening stands to be a time- and cost-effective method to facilitate daily stress recovery. Indeed, a recent meta-analysis of 104 randomized controlled trials on the effects of music concluded that music-based interventions have a positive impact on both physiological (d = .380, 95% CI [0.30–0.47]) and psychological (d = .545, 95% CI [0.43–0.66]) stress-related outcomes [11]. However, a large proportion of studies included in this meta-analysis were conducted in medical or therapeutic settings, and the included music-based interventions encompassed not only music listening but also music therapy. Thus, a more specific review to determine whether music listening alone is beneficial for the recovery of healthy individuals outside medical and therapeutic settings seemed justified.”

Introduction l.78: Why not include studies demonstrating effects on cortisol?

We have added studies demonstrating equivocal effects on cortisol to the sentence in question (page 4):

“Indeed, studies on music listening and stress recovery in healthy individuals are equivocal: although music listening is considered beneficial for physiological stress recovery, several studies have reported no differences in heart rate, heart rate variability, respiration rate, or blood pressure, or cortisol recovery between participants who listened to music and those who either sat in silence or listened to an auditory control [18-21].”

l.133: I would not recommend to write 'smaller amounts of salivary alpha-amylase'. Rather, less activity of alpha-amylase.

We agree that ‘less activity of alpha-amylase’ is more appropriate given what the outcome represents. We have replaced ‘amounts of salivary alpha-amylase’ accordingly (page 6):

“This manifests as a restoration of parasympathetic activity, marked by a deceleration of heart rate and respiration rate, lower systolic and diastolic blood pressure, and less activity of salivary alpha-amylase [4, 29-32].”

l.153: Study 54 refers to an ambulatory assessment study – therefore, there was no control to silence or noise in a comparable manner to experimental studies. Please re-word.

Thank you for pointing this out. We have adjusted the sentences accordingly (page 7):

“For example, music listening has been associated with lower heart rate [49-51], systolic blood pressure [22, 50, 52], skin conductance [18, 20, 53, 54], and cortisol [55] compared to silence or an auditory control condition. Similarly, participants who listened to music following stress demonstrated less activity of salivary alpha-amylase and lower cortisol compared to when music was listened to for other purposes [56].”

l.164: I do not agree with the statement that the candidate mechanism underlying beneficial effects of music has already been identified. I would rather prefer to see here a more comprehensive statement acknowledging that the exact underlying mechanisms remain to be elucidated and that different notions exist, e.g., literature by Koelsch…

Thank you for your suggestion. It was not our intention to suggest that a definite mechanism underlying the beneficial effects of music listening on stress recovery has been identified. We agree that a more comprehensive statement would help convey this point more clearly. Following your suggestion, we have adjusted the paragraph accordingly (page 7-8):

“The exact mechanisms underlying the effects of music listening on stress recovery remain to be elucidated. Music-evoked positive emotions are thought to be particularly beneficial for stress recovery, as they may help undo the unfavourable changes wrought by negative emotions during stress, ultimately aiding the stress recovery process (Tugade & Fredrickson, 2004). Alternatively, music-evoked emotions may promote a more robust, and thus more adaptive, stress response (Koelsch et al., 2016), which may be followed by an equally robust period of recovery. Next, it has been theorized that music may act as an anchor that draws attention away from post-stressor ruminative thoughts or negative affective states, thus preventing a lengthening of physiological activation, and facilitating a more regular stress recovery process (Baltazar et al., 2019; Radstaak et al., 2014). Finally, physiological rhythms in our body, such as respiration, cardiovascular activity, and electroencephalographic activity, may become fully or partially synchronized with rhythmical elements perceived in music (Ellis & Thayer, 2010; Trost et al., 2017). This rhythmic entrainment process is thought to occur via a bottom-up process that originates in the brainstem: salient musical features, such as tempo, pitch, and loudness, are continuously tracked by the brainstem, generating similar changes in ANS activity over time…”

Baltazar, M., & Saarikallio, S. (2019). Strategies and mechanisms in musical affect self-regulation: A new model. Musicae Scientiae, 23(2), 177-195. https://doi.org/10.1177/1029864917715061

Ellis, R. J., & Thayer, J. F. (2010). Music and autonomic nervous system (dys) function. Music perception, 27(4), 317-326. https://doi.org/10.1525/mp.2010.27.4.317

Koelsch, S., Boehlig, A., Hohenadel, M., Nitsche, I., Bauer, K., & Sack, U. (2016). The impact of acute stress on hormones and cytokines and how their recovery is affected by music-evoked positive mood. Scientific reports, 6(1), 1-11. https://doi.org/10.1038/srep23008

Radstaak, M., Geurts, S. A., Brosschot, J. F., & Kompier, M. A. (2014). Music and psychophysiological recovery from stress. Psychosomatic medicine, 76(7), 529-537. doi: 10.1097/PSY.0000000000000094

Trost, W. J., Labbé, C., & Grandjean, D. (2017). Rhythmic entrainment as a musical affect induction mechanism. Neuropsychologia, 96, 96-110. https://doi.org/10.1016/j.neuropsychologia.2017.01.004

Tugade, M. M., Fredrickson, B. L., & Barrett, L. F. (2004). Psychological resilience and positive emotional granularity: Examining the benefits of positive emotions on coping and health. Journal of personality, 72(6), 1161-1190. https://doi.org/10.1111/j.1467-6494.2004.00294.x

l.274: IgA is named as outcome measure but has not been introduced. As it is an immune marker, the introduction should contain some information on interactions among stress and immune system.

Thank you for the reminder. We have added an additional sentence in the Introduction to present salivary IgA as a marker for stress (page 5):

“This process enables rapid, non-genomic effects that sustain ANS-mediated changes for the duration of the stressor, while suppressing immune system function [33-35]. This suppression is visible through lower concentrations of immunoglobulins, such as salivary immunoglobulin-A (s-IgA; Chojnowska et al., 2021).”

Chojnowska, S., Ptaszyńska-Sarosiek, I., Kępka, A., Knaś, M., & Waszkiewicz, N. (2021). Salivary biomarkers of stress, anxiety, and depression. Journal of Clinical Medicine, 10(3), 517. https://doi.org/10.3390/jcm10030517

l.310: some typos need revision

Thank you for pointing this out. The typos have been revised (page 15):

“Disagreements were resolved through face-to-face discussions, or through consultation with SG and KR when no consensus could be reached.”

l.425: Can you please indicate the range of music duration? As there is literature available on the effects of different music durations on beneficial effects, I assume that the range was very limited among these studies. Therefore, I would not state that duration is not important. Rather, that the range in experimental studies is not vast enough to allow for meaningful comparisons.

We have added the range of music duration in our report of the moderator analyses (page 26):

“Duration of music. There was no evidence that the effect of music listening on stress recovery may differ depending on how long participants were exposed to music, β1 = -0.005, p = .870 (rangeduration = 2 – 45 minutes).”

Attachment

Submitted filename: ma20211025_rebuttal_PLOS_v1.docx

Decision Letter 1

Urs M Nater

5 Apr 2022

PONE-D-21-22806R1Music listening and stress recovery in healthy individuals: A systematic review with meta-analysis of experimental studiesPLOS ONE

Dear Dr. Adiasto,

Thank you for submitting your revised manuscript to PLOS ONE.  Both reviewers agree that your manuscript has greatly improved. One reviewer, however, has a few additional issues for you to consider. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

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Reviewer #2: (No Response)

**********

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**********

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Reviewer #1: The authors have satisfactorily addressed all my comments. I am satisfied with the manuscript in its current format, and recommend it for publication.

Reviewer #2: The authors clearly put a lot of work and time into the revisions, I highly appreciate that, and I think that the manuscript is now much stronger; I only have a few concerns left to be addressed:

1) When reading your response letter I considered it an excellent idea to combine both systematic review and meta-analytic approach. However, when I read the manuscript, I felt it overloaded the paper. Furthermore, I did not really understand, why the number of included studies varies among these two approaches (14 vs. 17 studies).

My suggestion would be to either start with describing the review approach and then calculate the overall meta-analytic effect or to move the systematic review to the Appendix (after having adjusted the analysis to the same number of studies included).

2) Thank you for describing in more detail how you operationalized unsuccesful stress induction. I am not entirely convinced by your approach. For example, you provide mean statistics for two studies and conclude that the mean difference represents a successful stress induction. At least, I would expect a citation backing this up or a statistical test considering mean and standard deviation. I wondered if it was more approproate to distinguish successful from unsuccessful and (third category) not reported. As for now, I would still argue to include only those studies with successful stress reduction (expecially given the unequal ratio that limits comparisons anyways).

3) Thank you for describign in more detail your inclusion criteria. As they do not cover 'music should have been played after the stressor', I still argue that the Thoma Paper should be included. Therefore, please change the inclusion criteria accordingly or include the paper. If you adjust the inclusion criteria, I would recommend to refer to the Thoma Paper in the discussion as time of intervention (before, during or after stressor) might be an important modulator.

4) I am sorry to read that you decided against EMA studies as I consider it a huge strength to combine both experimental and EMA evidence. I am not convinced that these two approaches should be studied separately, as they complement each other in a meaningful way. Also, I believe that including EMA studies would shed light on the heterogeneity as you have multiple time points and multiple contextual factors being repetatedly assessed over time. Nevertheless I accept your choice here, but recommend to acknowlegde EMA studies in the discussion (or outlook). Particularly as you describe in the introduction that music is so easily available, studying the mechanisms in daily life seems to be timely.

5) Please omit the following sentence from the manuscript as I am afraid that it does not reflect the findings on alpha-amylase appropriately.

Similarly, participants who listened to music following stress demonstrated less activity

of salivary alpha-amylase and lower cortisol compared to when music was listened to for other

purposes [56].”

**********

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Reviewer #1: Yes: Jasminka Majdandžić

Reviewer #2: No

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PLoS One. 2022 Jun 17;17(6):e0270031. doi: 10.1371/journal.pone.0270031.r004

Author response to Decision Letter 1


12 May 2022

----------------------------

Response to Reviewer #1

----------------------------

Dear Dr. Majdandžić,

Thank you for taking the time to review our revised manuscript. Given your expertise in the effects of music listening on stress and wound healing, we are grateful that you have recommended the manuscript for publication in its current form.

----------------------------

Response to Reviewer #2

----------------------------

Dear Reviewer,

Thank you for taking the time to review our revised manuscript. We once again appreciate your kind words on our work and are happy to read that you consider the manuscript to be stronger in its current form. Below, we address the additional issues you have raised and summarize the changes we have consequently made to our manuscript in a point-by-point fashion. You will first see your comments, followed by our responses and excerpts from our manuscript where applicable:

------

1) When reading your response letter I considered it an excellent idea to combine both systematic review and meta-analytic approach. However, when I read the manuscript, I felt it overloaded the paper. Furthermore, I did not really understand, why the number of included studies varies among these two approaches (14 vs. 17 studies).

My suggestion would be to either start with describing the review approach and then calculate the overall meta-analytic effect or to move the systematic review to the Appendix (after having adjusted the analysis to the same number of studies included).

Thank you for your suggestion. In one of the earliest drafts of our review, we chose to present the systematic review section prior to the meta-analysis. Thus, the meta-analysis served to quantify the extensive qualitative evidence we presented in the systematic review. In line with this approach, we initially decided that studies which did not (or could not) provide the necessary means and standard deviations to estimate effect sizes (in our case, Hedge’s g) would be excluded from the meta-analysis. However, since means and standard deviations are less relevant to a qualitative review, we decided that studies without means and standard deviations could still be included in the systematic review section instead of being excluded completely, provided they met the rest of our inclusion criteria. As such, in the previous version of our manuscript, 17 studies were part of the systematic review section, while only 14 of those studies were part of the meta-analysis section.

In the previous version of our manuscript, we attempted to briefly explain this through the following information:

“Based on these criteria, the final sample for the systematic review portion of our review consisted of 17 studies. Finally, for studies to be included in the meta-analysis portion of our review, means and standard deviations of stress recovery outcomes following stressor cessation must be available. Corresponding authors were contacted when this information was not available. When authors did not or could not provide the required information (e.g., due to data no longer being accessible), the outcome was dropped from the meta-analysis. Thus, following attempts to obtain missing information, the final sample for the meta-analysis portion of our review consisted of 14 studies.”

In the current version of our manuscript, the meta-analysis section is currently presented first, with the systematic review section painting a more detailed picture about the methodological heterogeneity between included studies. We understand that an extensive qualitative portion which directly follows a straight-forward quantitative synthesis can feel somewhat overwhelming. Thus, we have decided to follow your suggestion and moved the bulk of the systematic review portion to the Appendix of our manuscript.

To accommodate this change. we have adjusted the paragraph in our inclusion criteria as follows (page 11):

“Finally, for the purpose of the meta-analysis, means and standard deviations of stress recovery outcomes following stressor cessation must be available. Corresponding authors were contacted when this information was not available. When authors did not or could not provide the required information (e.g., due to data no longer being accessible), outcomes were dropped from the meta-analysis. Following attempts to obtain missing information, the final sample for our review consisted of 14 studies.”

Furthermore, we have added the following paragraph after the results of our moderator analyses, to direct readers’ attention towards the systematic review in the Appendix (page 26):

“To further illustrate the methodological heterogeneity among experimental studies on the effect of music listening on stress recovery, we provide a more extensive, qualitative overview of the included studies in Appendix C. A summary of this overview is presented in Table 4.”

------

2) Thank you for describing in more detail how you operationalized unsuccessful stress induction. I am not entirely convinced by your approach. For example, you provide mean statistics for two studies and conclude that the mean difference represents a successful stress induction. At least, I would expect a citation backing this up or a statistical test considering mean and standard deviation. I wondered if it was more appropriate to distinguish successful from unsuccessful and (third category) not reported. As for now, I would still argue to include only those studies with successful stress reduction (especially given the unequal ratio that limits comparisons anyways).

Thank you for mentioning your concern. In the two studies we have labelled ‘unsuccessful’ with regards to their stress induction procedures, the authors do hint at the success of their stressors in their respective manuscripts. However, since this success was not explicitly reported (e.g., through a comparison between baseline and post-stressor outcomes), we ultimately decided to label these as ‘unsuccessful.’

For example, in Gan et al. (2015), the authors cite a statistically significant paired-samples t-test comparing pre-stressor and post-music math anxiety in their no-music control group (only) as evidence that their stress induction procedure was successful for all their conditions. Fortunately, Gan et al. (2015) have reported pre- and post-stressor (i.e., pre-music) means and standard deviations for all our outcomes of interest. Thus, we were able to conduct our own paired-samples t-tests using pooled standard deviations, assuming a correlation of 0.5 between pre- and post-stressor outcome measures (Estrada et al., 2018). Our own t-tests indeed demonstrate that there is a significant increase in stress from which participants can recover from.

We were not able to employ a similar method for Labbé et al. (2007), as the authors did not explicitly report pre- and post-stressor means and standard deviations for our outcomes of interest. However, Labbé et al. (2007) presented the results of several F-tests with significant effects of time (under stress/pre-music vs. post-music) for all their conditions. Though these tests are not as accurate as a baseline vs. post-stressor comparison to evaluate the effects of stress induction procedures, we considered it plausible that a stress reaction had indeed occurred.

With these considerations in mind, we agree that labeling both studies as ‘unsuccessful’, with regards to their stress induction procedures, may not be the most correct decision. Thus, we have decided to follow your suggestion and change their coding to ‘unreported’ instead – in the sense that what the studies reported were not an explicit test of their stress induction procedures.

Our final consideration to keep these two studies with unreported stress induction procedures in the meta-analysis is that the estimated cumulative effect size excluding the two studies (i.e., comprising studies with successful stress induction only), g = 0.173, 95% CI [-0.26, 0.61], p = .399 (page 25):

“Stress induction checks. There were no significant differences in the effects of music listening on stress recovery for studies with successful (g = 0.173, 95% CI [-0.26, 0.61], p = .399) and unreported (g = 0.062, 95% CI [-0.08, 0.20], p = .115) stress induction checks, β1 = -0.108, p = .661.”

…does not significantly differ in magnitude or significance with the overall cumulative effect size including the two studies (i.e., comprising studies with successful and unreported stress induction), g = 0.15, 95% CI [-0.21, 0.52], p = 0.374 (page 23):

“Based on a meta-regression with RVE, the estimated overall effect of music listening on stress recovery was g = 0.15, 95% CI [-0.21, 0.52], t(13) = 0.92, p = 0.374.”

With regards to this matter, we have updated our discussion on page 38:

“Two studies with unreported stress induction procedures were still included in the review [17,84], as reported means for certain recovery outcomes still suggested an increase from baseline that participants could recover from. For example, with the information reported in Gan et al. [84], assuming a correlation of 0.5 between baseline and post-stressor measures of state anxiety, we estimated that their stress induction procedure elicited a significant increase in state anxiety in their sedative music (t(34) = 5.87, p < .001, mdiff = 8.17, SDdiff = 8.24), stimulative music (t(34) = 8.21, p < .001, mdiff = 12.42, SDdiff = 8.95), and control (t(34) = 13.15, p < .001, mdiff = 15.83, SDdiff = 7.12) conditions. As the overall estimated effect of music listening on the recovery process of healthy individuals following laboratory stressors may be relatively modest, it becomes particularly important to ensure that a sufficient stress response is elicited, to provide a larger window of opportunity in which the effect of music listening may be exerted on participants’ recovery processes. We thus encourage future studies to adopt validated, (variations of) well-known stress tasks, such as the TSST [109], SECPT [110], or CO2 stress task [111], which have been demonstrated to consistently elicit marked physiological and psychological stress-related responses in laboratory settings. Furthermore, we remind future studies to candidly report the results of their stress induction procedures to facilitate subsequent meta-syntheses of the effects of music listening on stress recovery.”

Estrada, E., Ferrer, E., & Pardo, A. (2019). Statistics for evaluating pre-post change: Relation between change in the distribution center and change in the individual scores. Frontiers in psychology, 9, 2696.

Gan, S. K. E., Lim, K. M. J., & Haw, Y. X. (2016). The relaxation effects of stimulative and sedative music on mathematics anxiety: A perception to physiology model. Psychology of Music, 44(4), 730-741.

Labbé, E., Schmidt, N., Babin, J., & Pharr, M. (2007). Coping with stress: the effectiveness of different types of music. Applied psychophysiology and biofeedback, 32(3), 163-168.

------

3) Thank you for describing in more detail your inclusion criteria. As they do not cover 'music should have been played after the stressor', I still argue that the Thoma Paper should be included. Therefore, please change the inclusion criteria accordingly or include the paper. If you adjust the inclusion criteria, I would recommend to refer to the Thoma Paper in the discussion as time of intervention (before, during or after stressor) might be an important modulator.

Thank you for pointing this out. We have revised our inclusion criteria to make it clearer that we elected to focus on studies looking at the effects of music listening on stress recovery after a stressor (page 11):

“Second, studies should compare music listening to silence or an auditory stimulus (e.g., white noise, audiobooks). To ensure that included studies tested the immediate effect of music listening on stress recovery, exposure to music, silence, or auditory stimuli must occur after the stress induction procedure.”

Next, given our focus, we agree that the timing of the music intervention may be an important moderator of the effects of music listening on stress recovery. Thus, we have added the following paragraph to our Discussion, referring to several studies investigating the effects of music listening at different timings, including the Thoma et al. (2013) paper (page 38-39):

“As the current review focused on the effects of music listening after a stressor, studies where music was played before or during a stressor were omitted from our analyses. However, several studies suggest that the timing at which music is played (i.e., before, during, or after a stressor) may influence its effects on stress recovery. For example, in Burns et al. [48], participants who listened to classical music while anticipating a stressful task exhibited lower post-music heart rate compared to participants who anticipated the stressor in silence. Similarly, concentrations of salivary cortisol were lower for participants who watched a stressful visual stimulus while listening to music compared to those who watched the same stimulus without music [112]. Together, these findings hint that, when listened during a stressor, music may attenuate cortisol responses [9,113], thus reducing the subsequent need for recovery. On the other hand, Thoma et al. [9] reported that participants who listened to music prior to a stressor exhibited higher post-stressor cortisol compared to participants who listened to an audio control. Interestingly, despite the stronger stress response, Thoma et al. [9] noted a trend for quicker ANS recovery among participants who listened to music, particularly with regards to salivary alpha-amylase activity. This pattern of findings is consistent with the notion forwarded by Koelsch et al. [61], in that music listening may promote a more adaptive stress response, thus facilitating subsequent stress recovery processes. To date, research on timing differences in the context of music listening and stress recovery is scarce. Thus, future studies could further examine the influence of such timing differences to better understand their role in the relationship between music listening and stress recovery.”

------

4) I am sorry to read that you decided against EMA studies as I consider it a huge strength to combine both experimental and EMA evidence. I am not convinced that these two approaches should be studied separately, as they complement each other in a meaningful way. Also, I believe that including EMA studies would shed light on the heterogeneity as you have multiple time points and multiple contextual factors being repeatedly assessed over time. Nevertheless, I accept your choice here, but recommend to acknowledge EMA studies in the discussion (or outlook). Particularly as you describe in the introduction that music is so easily available, studying the mechanisms in daily life seems to be timely.

Thank you for your explanation, and we apologize to not have been able to account for EMA studies at the present time. We have followed your suggestion and acknowledged the value of EMA studies in our Discussion section (page 39):

“Given the pervasiveness of stress, Ecological Momentary Assessment (EMA) studies may provide a more intimate outlook on the dynamics of daily music listening behaviour, particularly for the purpose of stress recovery. For example, through an ambulatory assessment study, Linnemann et al. (38) revealed that music produced the most notable reductions in physiological and psychological stress outcomes when it was listened to for the purpose of ‘relaxation’, compared to other reasons such as ‘distraction’, ‘activation’, and ‘reducing boredom’. Indeed, given their high ecological validity, EMA studies may provide further insight into important contextual variables in the relationship between music listening and stress recovery. For example, in an EMA study, listening to music in the presence of others was related to decreased subjective stress, attenuated cortisol secretion, and higher activity of salivary alpha-amylase (55). Furthermore, physiological responses to music may co-vary between members of a dyad when music is listened to by couples (114). Thus, given the benefits of EMA studies, we invite future studies to continue exploring the dynamics and contextual factors of music listening behaviour for stress recovery in daily life.”

------

5) Please omit the following sentence from the manuscript as I am afraid that it does not reflect the findings on alpha-amylase appropriately.

Similarly, participants who listened to music following stress demonstrated less activity

of salivary alpha-amylase and lower cortisol compared to when music was listened to for other purposes [56].”

Our apologies for not appropriately conveying the effects of music listening on salivary alpha-amylase. The sentence in question has been deleted. The paragraph now reads:

“For example, music listening has been associated with lower heart rate [48–50], systolic blood pressure [21,49,51], skin conductance [17,19,52,53], and cortisol [54,55] compared to silence or an auditory control condition. Furthermore, music listening has been associated with higher parasympathetic activity [56] compared to silence [3,37]. Together, these findings suggest that music listening may generate beneficial changes in ANS and HPA axis activity that should be conducive to the stress recovery process [27,57,58].”

Attachment

Submitted filename: ma20220426_rebuttal_PLOS.docx

Decision Letter 2

Urs M Nater

3 Jun 2022

Music listening and stress recovery in healthy individuals: A systematic review with meta-analysis of experimental studies

PONE-D-21-22806R2

Dear Dr. Adiasto,

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Kind regards,

Urs M Nater

Academic Editor

PLOS ONE

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Reviewers' comments:

Acceptance letter

Urs M Nater

9 Jun 2022

PONE-D-21-22806R2

Music listening and stress recovery in healthy individuals: a systematic review with meta-analysis of experimental studies

Dear Dr. Adiasto:

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on behalf of

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PLOS ONE

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