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. 2022 Jun 7;16(6):e0010474. doi: 10.1371/journal.pntd.0010474

Fig 4. Comparison of Wolbachia titres over culture period in relation to in vivo recovered parasites.

Fig 4

(A) Microfilariae release / female B. malayi at days 3, 7 and 14 after culturing in LEC bilayers (B) Wolbachia titres / B. malayi female removed from IL-4Rα-/-IL-5-/- mice or (week 0) or maintained in LEC bilayer co-cultures for 1 or 2 weeks (C) schematic of experimental groups: group 1; female B. malayi freshly isolated from in vivo infection (CB.17 SCID mice), group 2; female B. malayi 2-weeks after single sex implantation into CB.17 SCID mice, group 3; female B. malayi 2-weeks after culture in LCE bilayers. (D) MTT reductase activity and (E) Wolbachia titres / B. malayi female derived from groups 1–3. Horizontal bars represent medians with interquartile range. Data is derived from a single experiment of 6 cultures (A) combined of three experiments of 6 cultures (B) or a single experiment of 12 cultures (D,E). Statistical significance determined by Kruskal-Wallis one-way ANOVA with Dunn’s post-hoc tests are indicated ns; not significant, *P<0.05, **P<0.01 and ***P<0.001.