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. 2022 Jun 7;16(6):e0010474. doi: 10.1371/journal.pntd.0010474

Fig 5. Validation of B. malayi female culture system as an anti-Wolbachia drug screen.

Fig 5

(A) Schematic of anti-Wolbachia drug screen (B) Kaplein-Meier survival or (C) average motility of female B. malayi over 14 days to indicated exposures of doxycycline (DOX), TylAMac or vehicle (1% DMSO), (D) Anti-Wolbachia efficacy following 7 days or (E) 14 days of indicated exposures to DOX, TylAMac or vehicle. Data plotted is wsp copy numbers/Bm female determined by qPCR (D-E). Data is combined from two experiments with 6 culture wells initially containing 12 viable female B. malayi (for vehicle & DOX groups) or a single experiment of 6 culture wells for Flubentylosin groups. Horizontal bars represent median with interquartile range. Significance is indicated ***P<0.001, **P<0.01, *P<0.05 (Kruskal-Wallis with Dunn’s multiple comparison’s tests).