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. 2022 Jun 17;8(24):eabp9151. doi: 10.1126/sciadv.abp9151

Fig. 5. Fusion loop mutations affect full-length RABV-G conformation and trimerization.

Fig. 5.

(A) Confocal immunofluorescence of cell surface-displayed, full-length RABV-G with fusion loop point mutations, stained for total RABV-G expression (polyclonal) and prefusion RABV-G (RVA122). Images are displayed as maximum intensity projections. (B) Negative stain EM reconstructions of shed RABV-G (gray) purified via immunoprecipitation with RVA122 (green). (C and D) ELISA quantification of the amount of total shed RABV-G (C) or prefusion shed RABV-G (D) carrying fusion loop mutations relative to the WT. Statistical significance of differences between groups was analyzed using ANOVA. Error bars indicate SEM for three biological replicates. *P < 0.05; **P < 0.01.