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. 2022 Jun 17;8(24):eabn4564. doi: 10.1126/sciadv.abn4564

Fig. 3. Ovarian stromal cells of reproductively aged mice exhibit deficient mitochondrial bioenergetics that is improved by BGP-15 treatment.

Fig. 3.

(A) Ovarian stromal cells are isolated by microdissection of follicles and corpora lutea from the ovary followed by digestion and filtration. Low expression of granulosa cell (Amh and Fshr) and oocyte (Bmp15) markers is verified (B). Ovarian stromal cells were isolated from mice that were young, old (14 months), or old and treated with BGP-15 (100 mg/kg for 2 weeks in drinking water plus intraperitoneal injection for 4 days immediately before ovulation) followed by assessment of mitochondrial respiration (C), glycolysis (D), fatty acid oxidation (E), and ATP levels (F). Values represent means ± SEM of n = 3 experimental replicates, each using four to six wells containing cells pooled from three to five mice. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 by two-tailed t test (B and E) or one-way ANOVA (C, D, and F) or compared to vehicle-treated old mice.