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. 2022 Jun 17;8(24):eabn4564. doi: 10.1126/sciadv.abn4564

Fig. 5. Metformin and MitoQ reverse ovarian fibrosis in obese and reproductively old mice.

Fig. 5.

Obese (Ob) or reproductively old mice (12 months) were treated with metformin (Met) or MitoQ (MQ) via drinking water or untreated (Veh) and compared to lean/young (4 to 5 months old) controls. (A) Representative PSR-stained ovarian sections from obese mice and quantification of fibrosis area in the stroma (B). (C) Number of oocytes ovulated following gonadotropin stimulation of obese mice and controls. n = 35 (Lean), n = 42 (Obese), and n = 11 (Ob + Met and Ob + MQ). (D) Representative PSR-stained ovarian sections from old mice and quantification of stromal fibrosis area (E). (F) Number of oocytes ovulated in each group of reproductively old females. n = 44 (Old), n = 38 (Old + Met), and n = 44 (Old + MQ). Young females ovulated 21.0 ± 2.8 oocytes. (G) Preovulatory follicles were counted in ovarian sections of old females that failed to ovulate. (H) n = 19 (Old and Old + Met) and n = 17 (Old + MQ). Means ± SEM are shown. *P < 0.05, **P < 0.01, and ****P < 0.0001 by one-way ANOVA compared to lean/young controls or compared to untreated (Veh) mice as indicated (B, C, and E to G).