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. 2022 Jun 1;11:e70661. doi: 10.7554/eLife.70661

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© 2022, Dong et al

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Figure 7. — One example frame is chosen from the data, and the resulting images (top row) and contour plots (bottom row) are shown to demonstrate the effect of window size on background removal. The contour plots show the iso-contour of five intensity levels spaced linearly across the full intensity range of the corresponding image. Here, a window size of 20 pixels (middle column) is appropriate while both smaller and larger window sizes produce unsatisfactory results: a window size too small (left column) artificially limits the size of cells, and a window size too large (right column) does not remove the background effectively.