Table 1.
Various morphological and nuclear alterations in erythrocytes of Labeo rohita fish exposed to different pyriproxyfen concentrations.
| Parameters/days | Groups/treatment | |||
|---|---|---|---|---|
| T0 (0.0) | T1 (300 μg/L) | T2 (600 μg/L) | T3 (900 μg/L) | |
| Erythrocytes with lobed nucleus (%) | ||||
| 10 | 1.39 ± 0.08 | 1.42 ± 0.06 | 1.57 ± 0.09 | 3.43 ± 0.18∗ |
| 20 | 1.40 ± 0.02 | 1.43 ± 0.04 | 1.59 ± 0.08 | 3.45 ± 0.23∗ |
| 30 | 1.41 ± 0.03 | 1.47 ± 0.04 | 3.61 ± 0.09∗ | 4.46 ± 0.18∗ |
| Erythrocytes with blabbed nucleus (%) | ||||
| 10 | 1.22 ± 0.17 | 1.39 ± 0.22 | 1.54 ± 0.13 | 2.99 ± 0.12∗ |
| 20 | 1.24 ± 0.15 | 1.48 ± 0.17 | 1.57 ± 0.11 | 3.18 ± 0.09∗ |
| 30 | 1.29 ± 0.14 | 1.49 ± 0.16 | 2.64 ± 0.15∗ | 3.19 ± 0.07∗ |
| Erythrocytes with vacuolated nucleus (%) | ||||
| 10 | 2.36 ± 0.04 | 2.43 ± 0.02 | 2.63 ± 0.12 | 2.99 ± 0.06∗ |
| 20 | 2.38 ± 0.02 | 2.44 ± 0.04 | 2.65 ± 0.05 | 3.03 ± 0.09∗ |
| 30 | 2.40 ± 0.03 | 2.47 ± 0.04 | 3.69 ± 0.06∗ | 3.90 ± 0.16∗ |
| Notched nucleus (%) | ||||
| 10 | 1.79 ± 0.01 | 1.86 ± 0.03 | 2.92 ± 0.08 | 2.66 ± 0.10∗ |
| 20 | 1.80 ± 0.02 | 1.89 ± 0.02 | 1.95 ± 0.09 | 2.70 ± 0.07∗ |
| 30 | 1.81 ± 0.01 | 1.90 ± 0.03 | 2.67 ± 0.12∗ | 2.81 ± 0.09∗ |
| Binucleate nucleus (%) | ||||
| 10 | 1.44 ± 0.18 | 1.50 ± 0.13 | 1.64 ± 0.09 | 1.77 ± 0.05 |
| 20 | 1.48 ± 0.18 | 1.55 ± 0.05 | 1.71 ± 0.09 | 2.89 ± 0.09∗ |
| 30 | 1.49 ± 0.13 | 1.63 ± 0.07 | 3.88 ± 0.05∗ | 3.91 ± 0.08∗ |
| Pear shaped (%) | ||||
| 10 | 3.87 ± 0.34 | 4.04 ± 0.18 | 4.10 ± 0.14 | 7.07 ± 0.04∗ |
| 20 | 3.89 ± 0.31 | 4.19 ± 0.16 | 6.68 ± 0.32∗ | 7.16 ± 0.19∗ |
| 30 | 3.66 ± 0.21 | 4.20 ± 0.43 | 6.75 ± 0.28∗ | 7.19 ± 0.08∗ |
| Micronucleus (%) | ||||
| 10 | 1.73 ± 0.18 | 1.83 ± 0.19 | 2.24 ± 0.08∗ | 3.92 ± 0.08∗ |
| 20 | 1.74 ± 0.24 | 1.96 ± 0.09 | 2.58 ± 0.13∗ | 4.04 ± 0.05∗ |
| 30 | 1.75 ± 0.21 | 2.10 ± 0.13 | 3.13 ± 0.14∗ | 4.26 ± 0.06∗ |
| Condensed nucleus (%) | ||||
| 10 | 2.11 ± 0.57 | 2.24 ± 0.21 | 2.53 ± 0.66 | 3.41 ± 0.12∗ |
| 20 | 2.30 ± 0.33 | 2.35 ± 0.51 | 3.82 ± 0.16∗ | 4.30 ± 0.05∗ |
| 30 | 2.45 ± 0.48 | 2.47 ± 0.13 | 4.95 ± 0.69∗ | 6.25 ± 0.26∗ |
| Spindle shaped erythrocyte (%) | ||||
| 10 | 1.83 ± 0.21 | 2.09 ± 0.15 | 3.22 ± 0.19∗ | 4.40 ± 0.26∗ |
| 20 | 1.95 ± 0.19 | 2.16 ± 0.19 | 3.35 ± 0.14∗ | 4.88 ± 0.21∗ |
| 30 | 1.84 ± 0.34 | 2.23 ± 0.33 | 3.49 ± 0.17∗ | 5.12 ± 0.09∗ |
| Spherocytes (%) | ||||
| 10 | 1.94 ± 0.03 | 2.18 ± 0.17 | 3.46 ± 0.08∗ | 4.20 ± 0.23∗ |
| 20 | 1.98 ± 0.08 | 2.21 ± 0.15 | 3.56 ± 0.09∗ | 4.45 ± 0.16∗ |
| 30 | 2.01 ± 0.03 | 2.25 ± 0.09 | 3.78 ± 0.16∗ | 4.69 ± 0.31∗ |
In each row, values (Mean ± SE) bearing asterisks differ significantly (P ≤ 0.05) from that of values in untreated (T0 negative control) fish. T1, T2, and T3 are positive control and dose based.