Table 2.

Oxidative stress indices and antioxidant enzyme levels in liver tissues of Labeo rohita exposed to pyriproxyfen.

Parameters/days	Groups/treatment
	T0 (0.0)	T1 (300 μg/L)	T2 (600 μg/L)	T3 (900 μg/L)
ROS (optical density)
10	0.36 ± 0.03	0.37 ± 0.02	0.40 ± 0.03	0.84 ± 0.07∗
20	0.37 ± 0.01	0.39 ± 0.01	0.67 ± 0.02∗	0.86 ± 0.05∗
30	0.39 ± 0.04	0.42 ± 0.03	0.75 ± 0.02∗	0.92 ± 0.05∗
TBARS (nmol/TBARS formed/mg protein/min)
10	37.42 ± 2.93	39.23 ± 1.95	41.07 ± 1.15	54.93 ± 2.74∗
20	38.89 ± 1.94	40.68 ± 2.95	50.44 ± 1.97∗	56.25 ± 3.92∗
30	40.02 ± 3.94	41.95 ± 2.93	51.88 ± 2.34∗	58.83 ± 4.53∗
Reduced GSH (μmol/g tissue)
10	8.66 ± 1.17	7.65 ± 0.06	6.64 ± 0.13	5.64 ± 0.15∗
20	8.38 ± 1.12	7.44 ± 1.10	6.05 ± 0.02	5.56 ± 0.12∗
30	8.35 ± 1.14	7.38 ± 1.05	5.93 ± 1.15∗	5.48 ± 0.14∗
Antioxidant enzymes
SOD (units/mg protein)
10	11.65 ± 0.12	10.97 ± 0.12	10.29 ± 0.13	10.27 ± 0.11
20	11.64 ± 0.15	10.32 ± 0.15	9.46 ± 0.15	7.58 ± 0.14∗
30	10.75 ± 0.18	9.68 ± 0.18	7.10 ± 0.18∗	7.04 ± 0.17∗
CAT (units/min)
10	8.70 ± 0.19	7.52 ± 0.19	7.14 ± 0.19	5.16 ± 0.19∗
20	7.99 ± 0.17	7.35 ± 0.16	5.63 ± 0.16∗	5.03 ± 0.16∗
30	7.95 ± 0.16	7.02 ± 0.16	5.46 ± 0.16∗	4.95 ± 0.16∗
POD (units/μg)
10	4.08 ± 0.09	3.56 ± 0.09	2.78 ± 0.09∗	2.47 ± 0.08∗
20	4.03 ± 0.08	3.47 ± 0.08	2.63 ± 0.08∗	2.41 ± 0.08∗
30	3.96 ± 0.08	3.39 ± 0.08	2.55 ± 0.08∗	2.38 ± 0.08∗

In each row, values (Mean ± SE) bearing asterisks differ significantly (P ≤ 0.05) from that of values in untreated (T0 negative control) fish. T1, T2, and T3 are positive control and dose based.