Table 3.

Oxidative stress parameters and antioxidant enzyme levels in Labeo rohita kidneys tissues subjected to pyriproxyfen dosages.

Parameters/days	Groups/treatment
	T0 (0.0)	T1 (300 μg/L)	T2 (600 μg/L)	T3 (900 μg/L)
ROS (optical density)
10	0.56 ± 0.03	0.57 ± 0.01	0.63 ± 0.01	0.71 ± 0.02∗
20	0.53 ± 0.01	0.59 ± 0.02	0.65 ± 0.07∗	0.74 ± 0.03∗
30	0.57 ± 0.01	0.62 ± 0.02	0.70 ± 0.05∗	0.78 ± 0.07∗
TBARS (nmol/TBARS formed/mg protein/min)
10	28.51 ± 1.51	30.21 ± 1.11	35.92 ± 0.5∗	39.63 ± 1.39∗
20	29.08 ± 1.63	31.08 ± 1.16	37.07 ± 0.6∗	41.06 ± 1.83∗
30	29.72 ± 1.71	33.79 ± 1.17	37.79 ± 0.7∗	41.83 ± 1.77∗
Reduced GSH (μmol/g tissue)
10	7.70 ± 0.3	6.45 ± 0.3	5.21 ± 0.2∗	3.95 ± 0.2∗
20	7.66 ± 0.3	6.35 ± 0.2	5.18 ± 0.2∗	3.82 ± 0.2∗
30	7.54 ± 0.3	6.31 ± 0.2	5.10 ± 0.2∗	3.79 ± 0.2∗
Antioxidant enzymes
SOD (units/mg protein)
10	15.51 ± 0.36	13.53 ± 0.35	11.56 ± 0.33∗	9.55 ± 0.32∗
20	15.40 ± 0.36	13.14 ± 0.35	10.99 ± 0.33∗	8.77 ± 0.32∗
30	15.26 ± 0.36	13.10 ± 0.35	10.86 ± 0.33∗	8.68 ± 0.32∗
CAT (units/min)
10	5.12 ± 0.1	4.58 ± 0.09	3.84 ± 0.09∗	3.46 ± 0.07∗
20	5.08 ± 0.1	4.56 ± 0.09	3.78 ± 0.08∗	3.43 ± 0.07∗
30	5.04 ± 0.1	4.45 ± 0.09	3.62 ± 0.08∗	3.30 ± 0.06∗
POD (units/μg)
10	5.98 ± 0.13	5.33 ± 0.13	4.17 ± 0.11∗	4.04 ± 0.10∗
20	5.92 ± 0.13	5.30 ± 0.12	4.13 ± 0.11∗	3.95 ± 0.10∗
30	5.89 ± 0.13	5.27 ± 0.12	4.03 ± 0.10∗	3.89 ± 0.10∗

In each row, values (Mean ± SE) bearing asterisks differ significantly (P ≤ 0.05) from that of values in untreated (T0 negative control) fish. T1, T2, and T3 are positive control and dose based.