Table 4.

Oxidative stress parameters and antioxidant enzyme levels in Labeo rohita gills tissues subjected to pyriproxyfen dosages.

Parameters/days	Groups/treatment
	T0 (0.0)	T1 (300 μg/L)	T2 (600 μg/L)	T3 (900 μg/L)
ROS (optical density)
10	0.34 ± 0.03	0.37 ± 0.01	0.49 ± 0.07∗	0.55 ± 0.09∗
20	0.35 ± 0.05	0.39 ± 0.04	0.53 ± 0.06∗	0.57 ± 0.07∗
30	0.37 ± 0.04	0.41 ± 0.01	0.56 ± 0.08∗	0.59 ± 0.09∗
TBARS (nmol/TBARS formed/mg protein/min)
10	40.67 ± 1.63	44.47 ± 2.12	58.27 ± 2.22∗	61.07 ± 2.33∗
20	41.13 ± 2.61	44.88 ± 2.31	58.64 ± 3.41∗	62.39 ± 2.29∗
30	41.20 ± 1.61	45.04 ± 1.36	61.82 ± 2.39∗	63.70 ± 2.27∗
Reduced GSH (μmol/g tissue)
10	2.58 ± 0.05	2.24 ± 0.10	1.90 ± 0.04∗	1.56 ± 0.16∗
20	2.44 ± 0.11	2.23 ± 0.14	1.83 ± 0.03∗	1.52 ± 0.17∗
30	2.34 ± 0.15	2.05 ± 0.17	1.76 ± 0.08∗	1.47 ± 0.25∗
Antioxidant enzymes
SOD (units/mg protein)
10	10.79 ± 1.2	9.73 ± 1.12	8.98 ± 0.04	7.24 ± 0.12∗
20	10.68 ± 1.1	9.53 ± 1.17	8.13 ± 0.21∗	7.22 ± 0.18∗
30	10.57 ± 1.4	9.32 ± 1.11	8.07 ± 0.13∗	6.83 ± 0.31∗
CAT (units/min)
10	3.03 ± 0.14	2.89 ± 0.12	2.48 ± 0.08	2.19 ± 0.13∗
20	2.98 ± 0.11	2.79 ± 0.17	2.33 ± 0.08∗	2.15 ± 0.19∗
30	2.95 ± 0.09	2.73 ± 0.15	2.19 ± 0.14∗	2.08 ± 0.21∗
POD (units/μg)
10	0.41 ± 0.05	0.37 ± 0.01	0.36 ± 0.01	0.26 ± 0.03∗
20	0.40 ± 0.04	0.36 ± 0.02	0.30 ± 0.05∗	0.24 ± 0.04∗
30	0.39 ± 0.05	0.34 ± 0.04	0.29 ± 0.06∗	0.22 ± 0.02∗

In each row, values (Mean ± SE) bearing asterisks differ significantly (P ≤ 0.05) from that of values in untreated (T0 negative control) fish. T1, T2, and T3 are positive control and dose based.