Table 5.

Oxidative stress parameters and antioxidant enzyme levels in Labeo rohita brain tissue subjected to pyriproxyfen dosages.

Parameters/days	Groups/treatments
	T0 (0.0)	T1 (300 μg/L)	T2 (600 μg/L)	T3 (900 μg/L)
ROS (optical density)
10	0.48 ± 0.02	0.50 ± 0.02	0.51 ± 0.03	0.53 ± 0.03
20	0.46 ± 0.02	0.52 ± 0.02	0.59 ± 0.03∗	0.72 ± 0.04∗
30	0.51 ± 0.02	0.53 ± 0.03	0.66 ± 0.03∗	0.76 ± 0.04∗
TBARS (nmol/TBARS formed/mg protein/min)
10	18.60 ± 2.11	19.29 ± 1.03	20.97 ± 1.12	21.66 ± 1.06
20	19.08 ± 2.01	20.77 ± 1.21	26.46 ± 1.13∗	30.14 ± 1.13∗
30	19.18 ± 2.04	22.92 ± 1.5	26.66 ± 1.17∗	30.40 ± 2.13∗
Reduced GSH (μmol/g tissue)
10	3.01 ± 0.06	2.96 ± 0.06	2.79 ± 0.05	2.89 ± 0.04
20	2.96 ± 0.06	2.77 ± 0.05	2.66 ± 0.04	1.77 ± 0.03∗
30	2.88 ± 0.06	2.75 ± 0.05	2.10 ± 0.04∗	1.73 ± 0.03∗
Antioxidant enzymes
SOD (units/mg protein)
10	13.65 ± 0.3	12.09 ± 0.3	12.02 ± 0.2	11.96 ± 0.2
20	13.56 ± 0.3	12.20 ± 0.2	11.73 ± 0.2	8.87 ± 0.1∗
30	13.45 ± 0.3	11.88 ± 0.2	10.32 ± 0.2∗	8.75 ± 0.1∗
CAT (units/min)
10	4.16 ± 0.08	3.93 ± 0.08	3.85 ± 0.07	3.76 ± 0.06
20	4.12 ± 0.08	3.68 ± 0.08	3.77 ± 0.07	2.46 ± 0.06∗
30	4.07 ± 0.08	3.59 ± 0.07	2.98 ± 0.07∗	2.23 ± 0.06∗
POD (units/μg)
10	3.04 ± 0.06	2.79 ± 0.06	2.64 ± 0.05	2.59 ± 0.04
20	3.01 ± 0.06	2.73 ± 0.05	2.35 ± 0.05	1.98 ± 0.04∗
30	3.03 ± 0.06	2.69 ± 0.05	2.03 ± 0.05∗	1.94 ± 0.04∗

In each row, values (Mean ± SE) bearing asterisks differ significantly (P ≤ 0.05) from that of values in untreated (T0 negative control) fish. T1, T2, and T3 are positive control and dose based.