FIG. 2.

Comparison of the nucleotide sequences of the four attachment sites. attL and attR include, respectively, the left and right termini of var1C of strain CNRZ368. attB corresponds to the partial sequence of a PCR product obtained from strain A054 with the primers O132.3 and O131.2. attI corresponds to the partial sequence of a nested-PCR product obtained from strain CNRZ368 with the primer pairs O132.5-O131.5 and O132.4-O131.1 (Fig. 3). R1 sequences are written in capital letters. The italic letters correspond to the internal sequence of var1C. Underlined letters indicate the bases that are complementary to the 3′ end of the fda gene encoding fructose-1,6-biphosphate aldolase. Sequences underlined twice correspond to the HindIII restriction sites included in R1.