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. 2022 Jun 17;12:10163. doi: 10.1038/s41598-022-14602-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Estimation of the level of active molecules by EMSA. The Cy3-labeled DNA (0.5 µM) was incubated with varying amounts of hPolε_CD. Samples were separated by electrophoresis in 5% acrylamide gel and visualized by Typhoon FLA 9500. The percentage of DNA in the complex is plotted against the protein/DNA ratio, and the generated trend line shows the percentage of active molecules capable of DNA binding. The result of one representative experiment is shown.