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. 2022 Jun 17;13:3490. doi: 10.1038/s41467-022-31168-9

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Quantification of DAGLA and DAGLB mRNA expression by RNA-sequencing of LCM-isolated human nigral DAN samples (n = 26)²⁹. b Quantification of Dagla and Daglb mRNA expression by RNA-sequencing of LCM-isolated nigral DANs from adult mouse brains (n = 7). c Dagla and Daglb mRNA expression in the mouse dorsal striatum (DS, n = 6). The expression of Dagla and Daglb mRNAs was normalized by the expression of Th mRNAs in the nigral DANs and Bcl11b mRNAs in the SPNs. Data were presented as mean ± SEM. Unpaired t test, two-tailed, ^****p < 0.0001. d RNAscope^® in situ hybridization of Daglb and Th in mouse midbrain sections. Sections were counterstained with DAPI. Dashed line outlines the SNc region. Bottom panels highlight the boxed areas in the up panels. SNc: substantia nigra pars compacta. SNr: substantia nigra pars reticulata. VTA: ventral tegmental area. Scale bars: 100 μm (left) and 20 μm (right). More than five independent experiments were performed.