Fig. 7. JZL184 treatment promotes DAN activity and dopamine release.

a Schematic illustrates fiber photometry recording of GCaMP6f and tdT signals in the SNc of DAN-control and DAN-Daglb KD Dat^IRESCre/GCaMP6f/tdT trigenic mice. b Representative images of GCaMP6f (green), tDT (red), and TH (blue) staining. Scale bar: 200 μm. Six independent experiments were performed. c Alterations of calcium transients in the SNc of DAN-control and DAN-Daglb KD Dat^IRESCre/GCaMP6f/tdT trigenic mice (n = 3M per genotype) treated with vehicle or JZL184 (20 mg/kg). Data were presented as mean ± SEM. Multiple unpaired t test, two-tailed, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001. d Schematic illustrates fiber photometry recording of DA2m and tdT signals in the SN of DAN-control and DAN-Daglb KD mice. e Representative images of DA2m (green), tDT (red) and TH (magenta) staining. Scale bar: 200 μm. Nine independent experiments were performed. f Changes of dopamine release in the SN of DAN-control (n = 5 M) and DAN-Daglb KD (n = 4 M) mice treated with vehicle, JZL184 (20 mg/kg). Data were presented as mean ± SEM. Multiple unpaired t test, two-tailed, ^*p < 0.05, ^**p < 0.01.