Table 1.

Triatomine bloodmeal scores, number of samples with hosts identified, and detection of multiple bloodmeal hosts via next generation sequencing.

	n	~ 228 bp band on PCR	Sanger sequencing produced appropriately sized fragment that resulted in a BLAST match	Single host detected by NGS	Multiple hosts detected by NGS	Identifiable host results	95% confidence interval of identifiable host results
Bloodmeal status
Starved	71	27	10	7	3	14.1%	7.8–24.0%
Fed	44	29	26	24	2	59.1%	44.4–72.3%
Total	115	56	36	31	5	31.3%	23.6–40.3%
Collection status
Alive	71	35	23	20	3	32.4%	22.7–43.9%
Dead	44	21	13	11	2	29.5%	18.2–44.2%
Total	115	56	36	31	5	31.3%	23.6–40.3%

During dissection, triatomines were assigned bloodmeal scores of 1 (no blood, desiccated guts), 2 (no blood, guts visible), 3 (traces of blood in gut), 4 (blood present, but either not much or not fresh [dried]), or 5 (large amount of fresh blood); these were then classified as ‘starved’ (scores of 1–3) or fed (scores of 4 or 5). The herp1/herp1 primer set was used to amplify a 228 bp fragment of DNA (some samples also produced a second band of ~ 450 bp). Sanger sequencing and BLAST matching were used to determine bloodmeal hosts. Triatomines of ‘fed’ statuses more frequently had a bloodmeal host characterized via Sanger sequencing that triatomines of ‘starved’ statuses. In addition to Sanger sequencing, amplicons were subjected to next generation sequencing methods. Five triatomines had multiple hosts detected via next generation sequencing methods.