Skip to main content
. 2022 Jun 17;12:10234. doi: 10.1038/s41598-022-14208-8

Table 2.

Triatomine sample information for specimens with bloodmeal host result determined by direct Sanger and NGS methods.

Sample ID Triatoma species Collection Area T. cruzi status T. cruzi DTU Bloodmeal score Sanger sequencing Next generation sequencing
Sanger sequencing result E-value % Identity Next generation sequencing result Taxon assigned reads Relative abundance (%) E-value % Identity
Triatomines with only one bloodmeal host revealed AZ-011 T. gerstaeckeri Zoo—redfooted tortoise area Positive TcI/TcIV 4 Chelonoidis carbonaria 3.0E−93 98 Chelonoidis carbonaria 164,352 100 9.0E−104 100
AZ-036 T. gerstaeckeri Zoo—leopard area Positive TcIV 2 Rattus rattus 2.0E−94 99 Rattus rattus 309,811 100 9.0E−104 100
AZ-043 T. sanguisuga Zoo—wolf hybrid area Negative – 1 Gallus gallus 2.0E−94 98 Gallus gallus 226,082 100 9.0E−104 100
AZ-082 T. sanguisuga Zoo—lion area Negative – 4 Rattus rattus 5.0E−96 99 Rattus rattus 470,378 100 9.0E−104 100
AZ-084 T. gerstaeckeri Zoo—unknown exact area Negative – 5 Colobus spp 7.0E−95 98 Colobus guereza 453,712 100 9.0E−104 100
AZ-118 T. indictiva Zoo—tiger area Negative – 3 Panthera tigris 2.0E−79 96 Panthera tigris 91,503 100 9.0E−104 100
EL016 T. gerstaeckeri Near house with dog Positive TcI 3 Canis lupus familiaris 8.0E−69 93 Canis lupus familiaris 57,653 100 9.0E−104 100
PS195 T. gerstaeckeri Dog kennel Positive TcI 4 Canis lupus familiaris 9.0E−94 98 Canis lupus familiaris 511,553 100 9.0E−104 100
PS253 T. gerstaeckeri Dog kennel Positive TcI 5 Canis lupus familiaris 2.0E−96 97 Canis lupus familiaris 453,871 100 9.0E−104 100
PS334 T. sanguisuga On person outdoors Positive TcIV 5 Homo sapiens 1.0E−97 99 Homo sapiens 511,705 100 9.0E−104 100
PS403 T. sanguisuga In house with dog Positive TcIV 4 Canis lupus familiaris 6.0E−95 100 Canis lupus familiaris 471,446 100 9.0E−104 100
PS449 T. gerstaeckeri In house—in bed Positive TcI 5 Homo sapiens 3.0E−88 98 Homo sapiens 269,282 100 9.0E−104 100
PS498 T. gerstaeckeri Farm Positive TcI 4 Incilius nebulifer 3.0E−98 99 Incilius nebulifer 525,182 100 9.0E−104 100
PS501 T. gerstaeckeri On house on farm with sheep Positive TcI 2 Ovis aries 5.0E−86 97 Ovis aries 260,955 100 9.0E−104 100
PS503 T. gerstaeckeri Farm with sheep Positive TcI 4 Ovis aries 2.0E−85 97 Ovis aries 406,253 100 9.0E−104 100
PS507 T. gerstaeckeri Farm with goats Positive TcIV 4 Capra hircus 4.0E−87 98 Capra hircus 124,980 100 9.0E−104 100
PS675 Unknown (nymph) House Negative – 5 Homo sapiens 3.0E−93 99 Homo sapiens 475,763 100 9.0E−104 100
PS888 T. gerstaeckeri Farm with chickens Negative – 4 Gallus gallus 8.0E−89 98 Gallus gallus 536,574 100 9.0E−104 100
PS889 T. gerstaeckeri Farm with cats Negative – 3 Felis catus 1.0E−66 99 Felis catus 59,190 100 9.0E−104 100
PS1035 T. gerstaeckeri Unknown Positive TcIV 5 Canis lupus familiaris 1.0E−87 99 Canis lupus familiaris 357,283 100 9.0E−104 100
PS1102 T. gerstaeckeri Outside of house with dog Positive TcIV 5 Canis lupus familiaris 3.0E−93 98 Canis lupus familiaris 357,533 100 9.0E−104 100
PS1119 T. lecticularia Chicken coop Negative – 4 Gallus gallus 1.0E−87 98 Gallus gallus 272,724 100 9.0E−104 100
PS1122 T. gerstaeckeri House with dog Positive TcIV 4 Canis lupus familiaris 4.0E−97 99 Canis lupus familiaris 357,749 100 9.0E−104 100
PS1156 T. gerstaeckeri Dog bed Positive TcI/TcIV 4 Canis lupus familiaris 2.0E−96 100 Canis lupus familiaris 257,656 100 9.0E−104 100
PS1164 T. gerstaeckeri Farm with cattle Positive TcI 5 Bos taurus 5.0E−86 98 Bos taurus 326,466 100 9.0E−104 100
PS1256 T. indictiva House with dog Positive TcI 5 Canis lupus familiaris 2.0E−95 99 Canis lupus familiaris 481,273 100 9.0E−104 100
PS1257 T. gerstaeckeri Near dog Positive TcI/TcIV 4 Canis lupus familiaris 5.0E−91 98 Canis lupus familiaris 443,378 100 9.0E−104 100
PS1281 T. gerstaeckeri In house with dog Positive TcI 5 Canis lupus familiaris 3.0E−93 98 Canis lupus familiaris 500,946 100 9.0E−104 100
PS1301 T. lecticularia House Negative – 5 Homo sapiens 1.0E−91 99 Homo sapiens 340,185 100 9.0E−104 100
PS1306 T. sanguisuga House Negative – 5 Homo sapiens 1.0E−96 98 Homo sapiens 402,405 100 9.0E−104 100
SNBL005 T. gerstaeckeri Non-human primate research center Negative – 2 Odocoileus virginianus 7.0E−55 96 Odocoileus virginianus 8,453 100 4.0E−102 99
Triatomines with more than one bloodmeal host revealed AZ-040 T. indictiva Zoo—tiger area Negative – 1 Panthera tigris 2.0E−69 94 Panthera tigris 47,708 96.3 9.0E−104 100
Homo sapiens 1818 3.7 9.0E−104 100
AZ-085 T. sanguisuga Zoo—fox area Negative - 3 Incilius nebulifer 2.0E−48 92 Incilius nebulifer 1021 92.6 9.0E−104 100
Homo sapiens 36 3.3 9.0E−104 100
Canis lupus familiaris 24 2.2 9.0E−104 100
Sciurus carolinensis 22 2.0 4.0E−102 99
PS393 T. sanguisuga On outside of house Positive TcIV 4 Sciurus niger 1.0E−76 97 Sciurus niger 15,622 90.6 9.0E−104 100
Homo sapiens 1625 9.4 9.0E−104 100
PS502* T. gerstaeckeri Farm with sheep Positive TcI 2 Ovis aries 2.0E−75 94 Ovis aries 67,481 94.4 9.0E−104 100
Bassariscus astutus 4014 5.6 9.0E−104 100
PS706 T. sanguisuga On outside of house Positive TcIV 4 Sciurus carolinensis 8.0E−89 98 Sciurus carolinensis 247,455 99.3 4.0E−102 99
Procyon lotor 1762 0.7 9.0E−104 100

*998 reads in this sample identified Colobus guereza. The plausibility of this host in this sample was not verifiable and therefore, these were set aside from consideration.

Ecological data and vertebrate bloodmeal host identifications based on direct Sanger sequencing versus amplicon deep sequencing for 36 triatomines collected across Texas and Florida.