Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 17;13:3439. doi: 10.1038/s41467-022-31050-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a–h Representative tomographic slices of the repeating CatSper channel complexes arranged in zigzag-rows along the longitudinal axis of wild type mouse sperm flagella shown as cross-sectional (a, c, e, g) and top views (b, d, f, h). The number of zigzag-rows (arrowheads) varied from a single row (a, b), two rows (c, d), merging rows (e, f), to up to five rows (g, h). i–j A representative principal piece region of a whole-cell (i.e., not cryo-FIB milled) Efcab9^−/− mouse sperm flagellum (in non-capacitated state) viewed in cross-sectional (i) and longitudinal (j) tomographic slices. Labeled line in (i) indicates the position of the section shown in (j). Other labels: Ax axoneme, CPC central pair complex, DMT & 1-9 doublet microtubules, FS fibrous sheath, M membrane. k–t Representative tomographic slices from Efcab9^−/− sperm showing fragmented and short CatSper complex clusters with altered orientation relative to the flagellar axis (cross section views: k, m, o, q, s; top views: l. l’, n, p, r, r’, t). Two distinct arrangements are pseudo-colored (backslash (\) blue and forward slash (/) pink) in (l’ and r’). Black arrowheads in (r) indicate the interruptions of the row. u–x Zigzag-arrangement of CatSper in a human sperm flagellum shown in cross-sectional (u), top (v, w) and side view (x). Black arrowheads in (v and w) indicate the same position between the two slices show at slightly different angles. Scale bars, 100 nm.