Skip to main content
. 2022 Jun 17;13:3439. doi: 10.1038/s41467-022-31050-8

Fig. 6. Flagellar beating waveform of free-swimming sperm in four dimensions.

Fig. 6

a 4D flagellar beating waveform analyses of wild type and Efcab9−/− sperm by high-speed digital holographic microscopy (DHM). The time-lapse trace of a flagellum at 3D position (laboratory-fixed frame of reference xyz) is visualized in color and its projections onto xy- and xz-planes are shadowed in black. b 4D sperm trajectory analyses of wild type and Efcab9−/− sperm by DHM. The swimming trajectory of sperm is visualized by tracing the head position. ce Statistical analyses of head (c) and flagellar (d) xy-excursion, and flagellar z-excursion (e) from (a). n = 15 per group of three animals, a two-way repeated-measures ANOVA statistical test was performed, *p (0.0177) < 0.05, ***p < 0.001, p value below 0.001 cannot be given as exact value; the medians (thick dash lines) and interquartile ranges (thin dash lines) are indicated.