Figure 5.

DEP does not affect the activation of the SPS signalling pathway. (A) OD₆₀₀ and β-galactosidase activity in gap1Δ (Σ1278b background) with DEP at different phenylalanine concentrations (0, 2.5, 5 and 10 mM). Error bars indicate standard deviation (N = 2). Two-fold serial dilution of DEP (maximum concentration at 25 mM) was used. (B) Comparison of normalized growth and β-galactosidase activity in gap1Δ and gap1Δ ssy1Δ (Σ1278b background) with and without individual amino acids (5 mM) methionine (M), isoleucine (I), tyrosine (Y), threonine (T), tryptophan (W), leucine (L) and phenylalanine (F) at a series of DEP concentrations. Error bars indicate standard deviation (N = 2). Two-fold serial dilution of DEP (maximum concentration at 25 mM) was used. (One-tailed paired t-test: ****P ≤ 0.0001, ***P ≤ 0.001, **P ≤ 0.01, *P ≤ 0.05 and ns: P > 0.05).