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. 2022 Jun 17;12:10275. doi: 10.1038/s41598-022-14491-5

Figure 5.

Figure 5

NIN regulates extracellular matrix protein expression in high-passage CSMC. (A,B) Determination of collagen levels by SirCol quantitative assay. (A) Example of histology of Sirius Red staining of serum-stimulated CSMC (top; scale bar, 50 µm) and intracellular collagen levels in CSMC in serum-free (DMEM) or serum-treated conditions. (B) Increased supernatant collagen in CSMC cultures with serum or serum + NIN treatment. #p < 0.05 vs DMEM; *p < 0.05 vs serum alone. (C,D) Quantitative PCR analysis of mRNA for collagen I, III and V, fibronectin (C) or metalloproteinases (D; MMP1 and MMP13). CSMC were treated with FCS + NIN (1 µM) for 4, 8 or 48 h and data expressed relative to untreated controls. *p < 0.05 vs untreated control. (E,F) Absence of effect of TGFβ (1–50 ng/mL, 48 h) on CSMC growth (E) or total intracellular or secreted collagen (F).