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. 2022 Jun 16;221(8):e202203017. doi: 10.1083/jcb.202203017

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© 2022 Michaud et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 5. — RGA-3/4 recruitment to waves trails Rho activation and slightly leads peak of F-actin recruitment. All oocytes expressing untagged Ect2^ΔNLS and RGA-3/4^WT to generate cortical waves. (A) Frog oocyte expressing probe for active Rho (cyan; GFP-rGBD) and tagged RGA-3/4^WT (orange; RGA-3/4^WT-3xGFP). Kymographs (bottom) generated from 1-px line drawn at position ≫. Kymograph x scale also applies to still images; x scale bar = 20 µm; y scale bar = 5 min. (B) Representative intensity profile of active Rho and RGA-3/4^WT. (C) Cross-correlational analysis of cell in A showing 18-s delay between Rho activation and RGA-3/4^WT recruitment. (D) Frog oocyte expressing probe for F-actin (cyan; mCherry-UtrCH) and tagged RGA-3/4 (orange; RGA-3/4^WT-3xGFP). Kymographs (bottom) generated from 1-px line drawn at position ≫. Kymograph x scale also applies to still images; x scale bar = 20 µm; y scale bar = 5 min. (E) Representative intensity profile of F-actin and RGA-3/4^WT. (F) Cross-correlational analysis of cell in D showing 8-s delay between peak RGA-3/4^WT recruitment and peak F-actin signal.