Video 1.
Postmeiotic starfish oocytes expressing mCh-rGBD (cyan; right), excess WT Ect2, and varying doses of mNeon-RGA-3/4WT (orange; left): 25 ng/μl (needle concentration), 75 ng/μl, and 200 ng/μl. Corresponds to Fig. 1 C. Time-lapse confocal microscopy; time in min:s from start of recording; all are single superficial optical planes at 4-s intervals at 15 fps. Sequences highlight two key points: (1) RGA-3/4 recruits in the wake of Rho activity waves, and (2) increasing dose of RGA-3/4 modulates waves, first regularizing them before suppressing their amplitude and continuity. Scale bar = 50 µm.