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Frog embryo expressing RGA-3/4WT-3xGFP (orange) and a probe for F-actin (UtrCH; cyan) undergoing cytokinesis. Corresponds to Fig. 3 A. Time-lapse confocal microscopy; 11 s/frame at 20 fps. RGA-3/4 signal localizes to the cortex upon nuclear envelope breakdown and colocalizes at the contractile ring with F-actin during cytokinesis. Scale bar = 25 µm.
